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Alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male Murrah buffaloes

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Abstract

A supplement which ameliorates temperature-humidity menace in food producing livestock is a prerequisite to develop climate smart agricultural packages. A study was conducted to investigate the heat stress ameliorative efficacy of alpha lipoic acid (ALA) in male Murrah water buffaloes (Bubalus bubalis). Eighteen animals (293.61 ± 4.66Kg Bwt) were randomly allocated into three groups (n = 6); NHSC (non-heat-stressed control), HS (heat-stressed) and HSLA (heat-stressed-supplemented with ALA@32 mg/kg Bwt orally) based on the temperature humidity index (THI) and ALA supplementation. HS and HSLA were exposed to simulated heat challenge in a climatically controlled chamber (40 °C) for 21 consecutive days, 6 h daily. Physiological responses viz. Respiration rate (RR), Pulse rate (PR) and Rectal temperature (RT) were recorded daily before and after heat exposure. Blood samples were collected at the end of heat exposure on days 1, 6, 11, 16, and 21 and on day 28 (7th day post exposure which is considered as recovery) for peripheral blood mononuclear cells (PBMCs) separation, followed by RNA and Protein extraction for Real time quantitative PCR and Western blot analysis respectively, of heat shock proteins (HSPs). Two-way repeated measure ANOVA was performed between groups at different experimental periods. RR (post exposure) in HS and HSLA was significantly higher (P < 0.05) than NHSC from day 1 onwards but HSLA varied significantly from the HS 8th day onwards. Post exposure RT and PR in both HS and HSLA varied (P < 0.05) from NHSC throughout the study; but between HS and HSLA, RT significantly varied on initial 2 days and last 6 days (from days 16 to 21). HSP70 mRNA expression significantly up regulated in high THI groups with respect to the low THI group throughout the experimental period. During chronic stress (days 16 and 21) HSP70 significantly (P < 0.05) increased in HS but not in HSLA (P > 0.05) with respect to NHSC. ALA supplementation up-regulates and sustains (P < 0.05) the expression of HSP90 in HSLA in comparison to the HS and NHSC. HSP105 expression was significantly up-regulated (P < 0.05) in HS on days 16 and 21 (during long-term exposure) but only on day 21 (P < 0.05) in HSLA. HSP70, HSP90, and HSP105 protein expression dynamics were akin to the mRNA transcript data between the study groups. In conclusion, supplementing ALA ameliorates the deleterious effect of heat stress as reflected by improved physiological and cellular responses. ALA supplementation improved cellular antioxidant status and sustained otherwise easily decaying heat shock responses which concertedly hasten the baton change from a limited window of thermo tolerance to long run acclimatization.

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Funding

The National Initiative on Climate Resilient Agriculture (NICRA)—Indian Council of Agricultural Research (ICAR) project entitled “Adaptation strategies in livestock to thermal stress through nutritional and environmental manipulations” financially assisted to carry out the study.

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  1. Division of Physiology & Climatology, ICAR-IndianVeterinary Research Institute, Bareilly, Uttar Pradesh, India

    H. A. Samad, Y. Y. Konyak, A. Kumar, V. Verma, V. S. Chouhan, V. P. Maurya, Puneet Kumar, M. Sarkar & G. Singh

  2. Division of Pathology, ICAR-IndianVeterinary Research Institute, Bareilly, Uttar Pradesh, India

    S. K. Latheef

  3. Dolphin PG Institue of Biomedical & Natural Science, Dehradun, Uttarakhand, India

    I. A. Khan

  4. Division of Livestock economics and statistics, ICAR-IndianVeterinary Research Institute, Bareilly, Uttar Pradesh, India

    M. R. Verma

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Correspondence to G. Singh.

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Permission and ethical clearance for animal experimentation had been granted by Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of environment, Forest and climate change Government of India and institute animal ethics committee(IAEC) of the ICAR-Indian Veterinary Research Institute (IVRI), Izzatnagar, UP, India.

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Samad, H.A., Konyak, Y.Y., Latheef, S.K. et al. Alpha lipoic acid supplementation ameliorates the wrath of simulated tropical heat and humidity stress in male Murrah buffaloes. Int J Biometeorol 63, 1331–1346 (2019). https://doi.org/10.1007/s00484-019-01750-w

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