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In vitro evaluation of natural thermal mineral waters in human keratinocyte cells: a preliminary study

Abstract

We aimed to test the anti-inflammatory and angiogenic properties of two different thermal waters at the cellular level in human keratinocyte cells in the present study. Two different thermal waters, thermo-mineral BJ1 (Bursa, Turkey) and oligomineral BG (Bolu, Turkey), were tested in human keratinocyte (HaCaT) cell line. HaCaT cells were incubated for 3 days with thermal waters; RNA isolation was carried out in the treated and untreated cells. The gene expressions of TNFα, IL-1α, and VEGF were measured using the RT-qPCR. The tested thermal waters significantly decreased the expression of IL-1α (BJ1 93% p = 0.0024 and BG 38% p = 0.0303). BJ1 and BG thermal waters downregulated the expression of TNFα (59% p = 0.0001 and 23% p = 0.0238 respectively). Furthermore, BJ1 and BG significantly downregulated the gene expression of VEGF (98% p = 0.0430 and 15% p = 0.0120). The observed decrease in the gene expression of TNFα and IL1α could be interpreted as an anti-inflammatory effect of mineral waters on HaCaT cells. Moreover, the suppressed VEGF expression might be an indicator of the antiangiogenic effect on human keratinocytes. Therefore, we hypothesized that depending on their specific chemical composition such as silica (128 mg/L) in BJ1 and hydrogen sulfide (1.2 mg/L) in BG, thermal waters suppress pro-inflammatory cytokines and angiogenic growth factor. These preliminary findings might give insight on the underlying mechanisms of the therapeutic benefits observed in some skin diseases such as rosacea and psoriasis.

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References

  1. Beauvais F, Garcia-Mace JL, Joly F (1998) In vitro effects of Uriage spring water on the apoptosis of human eosinophils. Fundam Clin Pharmacol 12(4):446–450

    Article  CAS  Google Scholar 

  2. Carbajo JM, Maraver F (2017) Sulphurous mineral waters: new applications for health. Evid Based Complement Alternat Med 2017:8034084

  3. Celerier P, Litoux P, Dreno B, Richard A (1995) Modulatory effects of selenium and strontium salts on keratinocyte-derived inflammatory cytokines. Arch Dermatol Res 287(7):680–682

    Article  CAS  Google Scholar 

  4. Chebassier N, Ouijja el H, Viegas I, Dreno B (2004) Stimulatory effect of boron and manganese salts on keratinocyte migration. Acta Derm Venereol 84:191–194

    Article  CAS  Google Scholar 

  5. Chiarini A, Dal Pra I, Pacchiana R, Menapace L, Zumiani G, Zanoni M, Armato U (2006) Comano’s (Trentino) thermal water interferes with the expression and secretion of vascular endothelial growth factor-A protein isoforms by cultured human psoriatic keratinocytes: a potential mechanism of its anti-psoriatic action. Int J Mol Med 18(1):17–25

    Google Scholar 

  6. E.W. Rice Federation, WE and American Public Health Association (2012) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington, DC

    Google Scholar 

  7. Elsholz F, Harteneck C, Muller W, Friedland K (2014) Calcium-a central regulator of keratinocyte differentiation in health and disease. Eur J Dermatol 24(6):650–661

    CAS  Google Scholar 

  8. Federation WE, American Public Health Association (2005) Standard methods for the examination of water and wastewater. American Public Health Association (APHA), Washington, DC

    Google Scholar 

  9. Ferreira MO, Costa PC, Bahia MF (2010) Effect of São Pedro do Sul thermal water on skin irritation. Int J Cosmet Sci 32(3):205–210

    Article  CAS  Google Scholar 

  10. Ghersetich I, Brazzini B, Hercogova J, Lotti TM (2001) Mineral waters: instead of cosmetics or better than cosmetics? Clin Dermatol 194:478–482

    Article  Google Scholar 

  11. Joly F, Charveron M, Aries MF, Bidault J, Kahhak L, Beauvais F, Gall Y (1998) Effect of Avène spring water on the activation of rat mast cell by substance P or antigen. Skin Pharmacol Physiol 11(2):111–116

    Article  CAS  Google Scholar 

  12. Joly F, Branka JE, Lefeuvre L (2014) Thermal water from Uriage-les-Bains exerts DNA protection, induction of catalase activity and Claudin-6 expression on UV irradiated human skin in addition to its own antioxidant properties. J Cosmet Dermatol Sci Appl 4:99–106

    Google Scholar 

  13. Jung SH, Seo YK, Youn MY, Park CS, Song KY, Park JK (2009) Anti-aging and anti-inflammation effects of natural mineral extract on skin keratinocytes. Biotechnol Bioprocess Eng 14(6):861–868

    Article  CAS  Google Scholar 

  14. Lansdown AB (2002) Calcium: a potential central regulator in wound healing in the skin. Wound Repair Regen 10(5):271–285

    Article  Google Scholar 

  15. Lee HP, Choi YJ, Cho KA, Woo SY, Yun ST, Lee JT, Kim HJ, Lee KH, Kim JW (2012) Effect of spa spring water on cytokine expression in human keratinocyte HaCaT cells and on differentiation of CD4+ T cells. Ann Dermatol 24.3:324–336

    Article  CAS  Google Scholar 

  16. Prasad AS (2014) Zinc: an antioxidant and anti-inflammatory agent: role of zinc in degenerative disorders of aging. J Trace Elem Med Biol 28:364–371

    Article  CAS  Google Scholar 

  17. Seite S (2013) Thermal waters as cosmeceuticals: La Roche-Posay thermal spring water example. Clin Cosmet Investig Dermatol 6:23

    Article  Google Scholar 

  18. Staquet MJ, Peguet-Navarro J, Richard A, Schmitt D, Rougier A (2002) In vitro effect of a spa water on the migratory and stimulatory capacities of human Langerhans calls. Eur J Dermatol 12.4:LIX–LXI

    Google Scholar 

  19. Varga C, László M, Gerencsér G, Gyöngyi Z, Szendi K (2015) Natural UV-protective organic matter in thermal water. J Photochem Photobiol B Biol 144:8–10

    Article  CAS  Google Scholar 

  20. Verdy C, Branka JE, Lefeuvre L (2012) Modulation of sodium-dependent transporters expression in normal human keratinocytes by a sodium rich isotonic thermal water. J Cosmet Dermatol Sci Appl 2:254–262

    Google Scholar 

  21. Yang C, Yang Z, Zhang M, Dong Q, Wang X, Lan A, Zeng F, Chen P, Wang C, Feng J (2011) Hydrogen sulfide protects against chemical hypoxia-induced cytotoxicity and inflammation in HaCaT cells through inhibition of ROS/NF-κB/COX-2 pathway. PLoS One 6(7):e21971

    Article  CAS  Google Scholar 

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Acknowledgments

We would like to thank the provider of the samples of thermal waters: Bursa Jeotermal A.Ş., Bursa, Turkey, and Erpilic A.Ş., Bolu, Turkey.

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Correspondence to Müfit Zeki Karagülle.

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Karagülle, M.Z., Karagülle, M., Kılıç, S. et al. In vitro evaluation of natural thermal mineral waters in human keratinocyte cells: a preliminary study. Int J Biometeorol 62, 1657–1661 (2018). https://doi.org/10.1007/s00484-018-1565-8

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Keywords

  • Natural thermal waters
  • HaCaT
  • TNFα
  • IL-1α
  • VEGF