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Comparative expression profile of NOD1/2 and certain acute inflammatory cytokines in thermal-stressed cell culture model of native and crossbred cattle

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Abstract

Thermotolerance depends mainly on the health and immune status of the animals. The variation in the immune status of the animals may alter the level of tolerance of animals exposed to heat or cold stress. The present study was conducted to investigate the expression profile of two important nucleotide binding and oligomerization domain receptors (NLRs) (NOD1 and NOD2) and their central signalling molecule RIP2 gene during in vitro thermal-stressed bovine peripheral blood mononuclear cells (PBMCs) of native (Sahiwal) and crossbred (Sahiwal X HF) cattle. We also examined the differential expression profile of certain acute inflammatory cytokines in in vitro thermal-stressed PBMC culture among native and its crossbred counterparts. Results revealed that the expression profile of NOD1/2 positively correlates with the thermal stress, signalling molecule and cytokines. Present findings also highlighted that the expression patterns during thermal stress were comparatively superior among indigenous compared to crossbred cattle which may add references regarding the better immune adaptability of Zebu cattle.

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Acknowledgments

The authors are thankful to the Director, ICAR-CIRC Meerut for providing necessary facilities to carry out the present research. Authors also acknowledge the Science and Engineering Research Board, Government of India for providing financial support under the project YSS/2014/000279 to RD.

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Authors and Affiliations

  1. Molecular Genetics Laboratory, ICAR-Central Institute for Research on Cattle, Meerut, Uttar Pradesh, 250001, India

    V . Bhanuprakash, Umesh Singh, Gyanendra Singh Sengar, T. V. Raja, Rani Alex, Sushil Kumar, R. R. Alyethodi, Ashish Kumar, Ankur Sharma, Suresh Kumar & Rajib Deb

  2. School of Abiotic Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, India

    Basavraj Sajjanar

  3. Division of Animal Genetics and Breeding, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India

    Bharat Bhusan

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  1. V . Bhanuprakash
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  2. Umesh Singh
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  3. Gyanendra Singh Sengar
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  4. T. V. Raja
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  6. Rani Alex
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  7. Sushil Kumar
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  8. R. R. Alyethodi
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  9. Ashish Kumar
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  10. Ankur Sharma
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  11. Suresh Kumar
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  12. Bharat Bhusan
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  13. Rajib Deb
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Correspondence to V . Bhanuprakash or Rajib Deb.

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Institutional animal ethical permission has been taken for collection of blood samples.

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Bhanuprakash, V..., Singh, U., Sengar, G.S. et al. Comparative expression profile of NOD1/2 and certain acute inflammatory cytokines in thermal-stressed cell culture model of native and crossbred cattle. Int J Biometeorol 61, 931–941 (2017). https://doi.org/10.1007/s00484-016-1273-1

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  • DOI: https://doi.org/10.1007/s00484-016-1273-1

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