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Understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in crossbred bulls

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Abstract

Na+/K+-ATPase is an integral membrane protein composed of a large catalytic subunit (alpha), a smaller glycoprotein subunit (beta), and gamma subunit. The beta subunit is essential for ion recognition as well as maintenance of the membrane integrity. Present study was aimed to analyze the expression pattern of ATPase beta subunit genes (ATPase B1, ATPase B2, and ATPase B3) among the crossbred bulls under different ambient temperatures (20–44 °C). The present study was also aimed to look into the relationship of HSP70 with the ATPase beta family genes. Our results demonstrated that among beta family genes, transcript abundance of ATPase B1 and ATPase B2 is significantly (P < 0.05) higher during the thermal stress. Pearson correlation coefficient analysis revealed that the expression of ATPase Β1, ATPase B2, and ATPase B3 is highly correlated (P < 0.01) with HSP70, representing that the change in the expression pattern of these genes is positive and synergistic. These may provide a foundation for understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in cattle.

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Acknowledgments

The authors are thankful to the Director, ICAR-CIRC, Meerut and ICAR-NIASM, Baramati for providing the necessary facilities to conduct the present work. Authors are also thankful to the Military Farm, Meerut, Uttar Pradesh, India for providing experimental animals.

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Authors and Affiliations

  1. Molecular Genetics Laboratory, Division of Animal Genetics & Breeding, ICAR-Central Institute for Research on Cattle, Meerut, 250001, Uttar Pradesh, India

    Rajib Deb, Umesh Singh, Rani Alex, T. V. Raja, Rafeeque R. Alyethodi, Sushil Kumar, Gyanendra Sengar, Sheetal Sharma, Rani Singh & B. Prakash

  2. School of Atmospheric Stress Management, ICAR-National Institute of Abiotic Stress Management, Baramati, Maharashtra, India

    Basavaraj Sajjanar

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  1. Rajib Deb
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  2. Basavaraj Sajjanar
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  3. Umesh Singh
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  4. Rani Alex
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  5. T. V. Raja
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  6. Rafeeque R. Alyethodi
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  7. Sushil Kumar
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  8. Gyanendra Sengar
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  9. Sheetal Sharma
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  10. Rani Singh
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  11. B. Prakash
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Correspondence to Rajib Deb.

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Deb, R., Sajjanar, B., Singh, U. et al. Understanding the mechanisms of ATPase beta family genes for cellular thermotolerance in crossbred bulls. Int J Biometeorol 59, 1783–1789 (2015). https://doi.org/10.1007/s00484-015-0986-x

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  • DOI: https://doi.org/10.1007/s00484-015-0986-x

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