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Cold exposure inhibits hypothalamic Kiss-1 gene expression, serum leptin concentration, and delays reproductive development in male Brandt’s vole (Lasiopodomys brandtii)

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Abstract

Cold commonly affects growth and reproductive development in small mammals. Here, we test the hypothesis that low ambient temperature will affect growth and puberty onset, associated with altered hypothalamic Kiss-1 gene expression and serum leptin concentration in wild rodents. Male Brandt’s voles (Lasiopodomys brandtii) were exposed to cold (4 ± 1 °C) and warm (23 ± 1 °C) conditions from the birth and sacrificed on different developmental stages (day 26, day 40, day 60, and day 90, respectively). Brandt’s voles increased the thermogenic capacity of brown adipose tissue, mobilized body fat, decreased serum leptin levels, and delayed the reproductive development especially on day 40 in the cold condition. They increased food intake to compensate for the high energy demands in the cold. The hypothalamic Kiss-1 gene expression on day 26 was decreased, associated with lower wet testis mass and testis testosterone concentration on day 40, in the cold-exposed voles compared to that in the warm. Serum leptin was positively correlated with body fat, testis mass, and testosterone concentration. These data suggested that cold exposure inhibited hypothalamic Kiss-1 gene expression during the early stage of development, decreased serum leptin concentration, and delayed reproductive development in male Brandt’s voles.

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Abbreviations

COX:

Cytochrome c oxidase

ECL:

Enhanced chemiluminescence

EWAT:

Epididymal white adipose tissue

iBAT:

Inter-scapular brown adipose tissue

RIA:

Radioimmunoassay

RU:

Relative unit

UCP1:

Uncoupling protein 1

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Acknowledgments

Thanks to all the members of the Animal Physiological Ecology Group for helpful comments on the manuscript and their help for the experiment. Thanks Yupeng Li for his help in the experiment. We thank Yanqin Liu for help to take care of the animals. This research was supported by grants from the National Natural Science Foundation of China (No. 31270010, 30900175 and 31272312) to XYZ and DHW.

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Authors and Affiliations

  1. State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, 100101, Beijing, China

    Qiang Zhang, Yi Lin, Xue-Ying Zhang & De-Hua Wang

  2. Department of Biology, Zunyi Normal College, 563002, Zunyi, China

    Qiang Zhang

  3. University of the Chinese Academy of Sciences, 100049, Beijing, China

    Yi Lin

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  1. Qiang Zhang
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  2. Yi Lin
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Correspondence to De-Hua Wang.

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Qiang Zhang and Yi Lin contributed equally to this work.

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Zhang, Q., Lin, Y., Zhang, XY. et al. Cold exposure inhibits hypothalamic Kiss-1 gene expression, serum leptin concentration, and delays reproductive development in male Brandt’s vole (Lasiopodomys brandtii). Int J Biometeorol 59, 679–691 (2015). https://doi.org/10.1007/s00484-014-0879-4

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  • DOI: https://doi.org/10.1007/s00484-014-0879-4

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