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International Journal of Biometeorology

, Volume 58, Issue 7, pp 1657–1663 | Cite as

Impact of 900 MHz electromagnetic field exposure on main male reproductive hormone levels: a Rattus norvegicus model

  • Masood SepehrimaneshEmail author
  • Mehdi Saeb
  • Saeed Nazifi
  • Nasrin Kazemipour
  • Gholamali Jelodar
  • Saeedeh Saeb
Original Paper

Abstract

This work analyzes the effects of radiofrequency-electromagnetic field (RF-EMF) exposure on the reproductive system of male rats, assessed by measuring circulating levels of FSH, LH, inhibin B, activin B, prolactin, and testosterone. Twenty adult male Sprague–Dawley rats (180 ± 10 g) were exposed to 900 MHz RF-EMF in four equal separated groups. The duration of exposure was 1, 2, and 4 h/day over a period of 30 days and sham-exposed animals were kept under the same environmental conditions as the exposed group except with no RF-EMF exposure. Before the exposure, at 15 and 30 days of exposure, determination of the abovementioned hormone levels was performed using ELISA. At the end of the experiment, FSH and LH values of the long time exposure (LTE) group were significantly higher than the sham-exposed group (p < 0.05). Serum activin B and prolactin in the LTE group showed significant increase and inhibin B showed significant decrease than sham and short time exposed (STE) groups after 30 days RF-EMF exposure (p < 0.05). Also, a significant decrease in serum testosterone levels in the LTE group was found compared to short and moderate time exposed (MTE) groups after 30 days RF-EMF exposure (p < 0.05). Results suggest that reproductive hormone levels are disturbed as a result of RF-EMF exposure and it may possibly affect reproductive functions. However, testosterone and inhibin B concentrations as a fertility marker and spermatogenesis were decreased significantly.

Keywords

GSM Testosterone Inhibin B Activin B Electromagnetic field Leydig cells Sertoli cells 

Notes

Acknowledgments

Animals were kindly provided by Dr Mahjoob Vahedi at the Laboratory Animal Center of University of Medical Science, Shiraz, Iran. Hormonal analysis was kindly performed with the cooperation of Professor Saeb Specialized Hormone Laboratory, Shiraz, Iran. Mr. Omid Koohi Hosseinabadi provided expert technical assistance with animal handling and sampling. The authors declare that there is no conflict of interest that would prejudice the impartiality of this scientific work.

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Copyright information

© ISB 2013

Authors and Affiliations

  • Masood Sepehrimanesh
    • 1
    Email author
  • Mehdi Saeb
    • 1
  • Saeed Nazifi
    • 2
  • Nasrin Kazemipour
    • 1
  • Gholamali Jelodar
    • 3
  • Saeedeh Saeb
    • 4
  1. 1.Department of Biochemistry, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Clinical Pathology, School of Veterinary MedicineShiraz UniversityShirazIran
  3. 3.Department of Physiology, School of Veterinary MedicineShiraz UniversityShirazIran
  4. 4.Department of Clinical Biochemistry, School of MedicineShiraz University of Medical SciencesShirazIran

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