GSNOR deficiency enhances betulin production in Betula platyphylla
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This paper showed that GSNOR mediated betulin production from genetic and pharmacological levels.
The aim of this study was to investigate the relationship between S-nitrosoglutathione reductase (GSNOR) and betulin production. Treatment of birch suspension cells with 20 μmol/L GSNOR inhibitor 3-(5-(4-(1H-imidazol-1-yl) phenyl)-1-(4-carbamoyl-2-methylphenyl)-1H-pyrrol-2-yl) propio- nic acid (N6022) markedly reduced gene expression of GSNOR, and increased at least two times in betulin content and gene expression of lupeol synthase (LUS), a key enzyme in betulin biosynthesis. GSNOR transgenic plants by RNAi silencing also lowered gene expression of GSNOR and increased betulin content and gene expression of LUS. Our study also showed that S-nitrosothiols (SNO) content increased in birch suspension cells treated with N6022 and GSNOR transgenic birch plants, about three times that in the non-transgenic birch. The above results verified that GSNOR deficiency mediated betulin production from genetic and pharmacological levels, and indicated that protein S-nitrosylation mediated plant secondary metabolite production.
KeywordsBetula platyphylla GSNOR SNO Betulin
This work was supported by the Fundamental Research Funds for the Central Universities (2572017EA05), Heilongjiang Natural Science Foundation of China (C2016005), and Harbin Technological Innovation Special Fund research Projects (2014RFQXJ066).
Author contribution statement
Guizhi Fan and Yaguang Zhan conceived and designed the experiments. Tingting Nie and Yating Huang performed the experiments and collected the data. Yating Huang analyzed the data. Guizhi Fan wrote the paper.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Barroso JB, Corpas FJ, Carreras A, Rodríguez-Serrano M, Esteban FJ, Fernández-Ocaña A, Chaki M, Romero-Puertas MC, Valderrama R, Sandalio LM, del Río LA (2006) Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress. J Exp Bot 57(8):1785–1793CrossRefPubMedGoogle Scholar
- Chaki M, Valderrama R, Fernández-Ocaña AM, Carreras A, Gómez-Rodríguez MV, Pedrajas JR, Begara-Morales JC, Sánchez-Calvo B, Luque F, Leterrier M, Corpas FJ, Barroso JB (2011) Mechanical wounding induces a nitrosative stress by down-regulation of GSNO reductase and an increase in S-nitrosothiols in sunflower (Helianthus annuus) seedlings. J Exp Bot 62(6):1803–1813CrossRefPubMedGoogle Scholar
- Fan GZ, Nie TT, Fan JS, Zhan YG (2017) Exogenous feeding of fructose and phenylalanine further improves Betulin Production in suspended Betula platyphylla Cells under Nitric Oxide Treatment. Molecules 22(1035):4–11Google Scholar
- Martínez MC, Achkor H, Persson B, Fernández MR, Shafqat J, Farré J, Jörnvall H, Parés X (1996) Arabidopsis formaldehyde dehydrogenase. Molecular properties of plant class III alcohol dehydrogenase provide further insights into the origins, structure and function of plant class p and liver class I alcohol dehydrogenases. Eur J Biochem 241:849–857CrossRefPubMedGoogle Scholar
- Zeng FS, Sun FK, Li LL, Liu K, Zhan YG (2014) Genome-scale transcriptome analysis in response to nitric oxide in birch cells: implications of the triterpene biosynthetic pathway. PLoS ONE 9:116–157Google Scholar