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Overexpression of ThGSTZ1 from Tamarix hispida improves tolerance to exogenous ABA and methyl viologen

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Molecular analysis of a zeta subfamily GST gene from T. hispida involved in ABA and methyl viologen tolerance in transgenic Arabidopsis and Tamarix.

Abstract

Glutathione S-transferase (GST) genes are important for the improvement of plant abiotic stress tolerance, and our previous study demonstrated that the ThGSTZ1 gene from Tamarix hispida improves plant salt and drought tolerance. To further understand the role of ThGSTZ1 in the response of plants to abscisic acid (ABA) and oxidative stress, three ThGSTZ1-overexpressing transgenic Arabidopsis thaliana lines were analyzed in the current study. The results showed that the transgenic lines exhibited higher biomass accumulation, higher activities of GST and other protective enzymes, and less reactive oxygen species (ROS) and cell damage than wild-type (WT) plants under ABA and methyl viologen (MV) stress. In addition, the analysis of a transgenic T. hispida line transiently expressing ThGSTZ1 confirmed these results. The activities of GST, glutathione peroxidase, and superoxide dismutase were markedly higher in the ThGSTZ1-overexpressing lines compared with the control lines under both ABA and MV treatments, and the transgenic lines also exhibited a lower degree of electrolyte leakage (EL) and a decreased H2O2 content. All these results suggested that ThGSTZ1 can also improve plant ABA and oxidation tolerance by regulating ROS metabolism and that ThGSTZ1 represents an excellent candidate gene for molecular breeding to increase plant stress tolerance.

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Acknowledgments

This work was supported by  the Fundamental Research Funds for the Central Universities (2572014DA03), the National Natural Science Foundation of China (No. 31370676), the Scientific Research Fund of Heilongjiang Provincial Education Department (No. 12523017), and the Postdoctoral Science Foundation of Heilongjiang Province (No. LBH-Q10155).

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Authors and Affiliations

  1. State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, 26 Hexing Road, 150040, Harbin, People’s Republic of China

    Caiqiu Gao, Guiyan Yang, Yucong Guo, Yulin Zhao & Chuanping Yang

  2. Laboratory of Walnut Research Center, Walnut Experimental Station, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Guiyan Yang

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  1. Caiqiu Gao
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  2. Guiyan Yang
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  3. Yucong Guo
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  4. Yulin Zhao
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  5. Chuanping Yang
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Correspondence to Chuanping Yang.

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Communicated by J. Carlson.

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Gao, C., Yang, G., Guo, Y. et al. Overexpression of ThGSTZ1 from Tamarix hispida improves tolerance to exogenous ABA and methyl viologen. Trees 30, 1935–1944 (2016). https://doi.org/10.1007/s00468-016-1422-5

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