Intraspecific variation of anatomical and chemical defensive traits in Maritime pine (Pinus pinaster) as factors in susceptibility to the pinewood nematode (Bursaphelenchus xylophilus)
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Migration ability of the PWN through wood branch tissues of adult Maritime pine trees significantly differed among Iberian provenances and this variation was related to differences in anatomical and chemical defensive traits.
The pinewood nematode or pine wilt nematode (PWN; Bursaphelenchus xylophilus) is one of the most dangerous threats to European coniferous forests, especially for the susceptible Maritime pine (Pinus pinaster), a valuable forest resource in South Western Europe. The PWN is vectored by beetles of the genus Monochamus (Coleoptera, Cerambycidae) and once inoculated in healthy branches, it quickly migrates downward to the main trunk through the resin canal system. Therefore, the anatomy of the resin canal system may modulate its migration and proliferation rates. Using material from nine Maritime pine Iberian provenances established in a common garden trial, we investigated whether these provenances differed in their (1) resin canal anatomy, (2) concentration of chemical defences (non-volatile resin and total polyphenolics) in stems and (3) ability of the PWN to migrate through the pine woody tissues in ‘in vitro’ bioassays. Whether variation in anatomical and chemical defensive traits relates to differences in PWN migration across populations was also investigated. Significant intraspecific variation in anatomical and chemical defensive traits and in nematode migration rates through pine tissues was observed. Moreover, the variation in nematode migration rate among pine provenances was related to differences in both anatomical and chemical features. Overall, this study highlights the role of plant genetics in the development of defensive traits against this harmful coniferous pest. The observed intraspecific variation should be taken into account when considering breeding as a strategy to provide areas of high risk of PWN with resistant genetic material.
KeywordsAnatomical defences Nematode migration rate Non-volatile resin Pine wilt disease (PWD) Polyphenolics Population differentiation Resin canals
Author contribution statement
LS, RZ and MV conceived the study. LS and XM conducted the sampling and field assessments. XM performed the chemical analyses and the histological analyses with the assistance of LS and AS. MR and ML performed the PWN migration bioassays with the assistance of MNS. RZ performed the statistical analyses, and primarily wrote the manuscript. All authors contributed to the writing and revisions.
This study was financed by the Spanish National Research Grants AGL2012-40151 (FENOPIN), co-financed by EU-FEDER, and by the bilateral action Spain-Portugal PRI-AIBPT-2011-1152 (NEMARES). The Portuguese Fundo Florestal Permanente, Instituto de Financiamento da Agricultura e Pescas I.P., Autoridade Florestal Nacional is also acknowledged for funding. We thank Manuel Mota (Universidade de Évora, Portugal) for providing B. xylophilus isolates. The genetic trials in which the samples were collected are part of the experimental set up of the Maritime pine breeding program developed by the Centro de Investigación Forestal de Lourizán, Xunta de Galicia. We thank David Brown for language edition, and Alvin Yanchuk for valuable comments and discussion.
Conflict of interest
The authors declare that they have no conflict of interest.
- de la Mata R, Zas R (2010a) Performance of maritime pine Spanish Mediterranean provenances in a transitional region between Atlantic and Mediterranean climates in NW Spain. Silvae Genet 59:8–17Google Scholar
- Dwinell LD (1984) Relative susceptibilities of 5 southeastern pine species to the pinewood nematode, Bursaphelenchus xylophilus. Phytopathology 74:870Google Scholar
- Evans H, Futai K (2008) Ecology and modeling. In: Vieira P, Mota M (eds) Pine wilt disease: a worldwide threat to forest ecosystems. Springer, Germany, pp 255–257Google Scholar
- Fernández JM, Solla A (2008) Mapas de riesgo de aparición y desarrollo de la enfermedad del marchitamiento de los pinos (Bursaphelenchus xylophilus) en Extremadura. For Syst 15:141–151Google Scholar
- Lima M, Ramos M, Sampedro L, Moreira X, Zas R, Vasconcelos M (2012) Teste de susceptibilidade ao nematodo da madeira do pinheiro–optimizaçao para utilizaçao en larga escala. Cuad Soc Esp Cien For 36:21–26Google Scholar
- Littell RC, Milliken GA, Stroup WW, Wolfinger RD, Schabenberger O (2006) SAS System for mixed models, 2nd edn. SAS Institute, CaryGoogle Scholar
- Ruzin SE (1999) Plant microtechnique and microscopy. Oxford University Press, New YorkGoogle Scholar
- Tadesse W, Auñón FJ, Pardos JA, Gil L, Alía R (2001) Evaluación precoz de la producción de miera en Pinus pinaster Ait. Inv Agr Sist Rec For 10:141–150Google Scholar
- Toda T, Kurinobu S (2002) Realized genetic gains observed in progeny tolerance of selected Red pine (Pinus densiflora) and Black pine (P. thunbergii) to pine wilt disease. Silvae Genet 51:42–44Google Scholar
- Webster J, Mota M (2008) Pine wilt disease: global issues, trade and economic Impact. In: Mota M, Vieira P (eds) Pine wilt disease: a worldwide threat to forest ecosystems. Springer, pp 1-5Google Scholar
- Zas R, Merlo E (2008) El programa de mejora de Pinus pinaster en Galicia. Boletin CIDEU 5–6:5–24Google Scholar