Trees

, Volume 29, Issue 2, pp 563–574 | Cite as

Extracellular ATP is involved in the initiation of pollen germination and tube growth in Picea meyeri

Original Paper

Abstract

Key message

Gymnosperm pollen grains release ATP to the extracellular matrix which is essential for the initiation of pollen germination and tube growth.

Abstract

Extracellular ATP (eATP) is an important signaling compound involved in various processes of animal and angiosperm cells. However, the role played by eATP in gymnosperm cells remains unclear. Using a bioluminescence assay, we found that pollen grains of Picea meyeri released ATP to the extracellular matrix before germination and during tube elongation. The addition of further exogenous ATP or an apyrase inhibitor to pollen suspensions inhibited germination and pollen tube elongation. Exogenous apyrase (which hydrolyzes eATP released from pollen per se) exerted a similar inhibitory effect. Moreover, incubation of pollen suspensions with purinoceptor inhibitors prevented germination. ATP intensified the influx of Ca2+ after germination, which was abrogated by purinoceptor inhibitors. Confocal microscopy revealed that the microfilament pattern became disorganized in pollen tubes when exposed to ATP. Together, our findings suggest that optimum concentration of eATP is essential for initiation of pollen germination, and eATP signaling regulates pollen tube growth by activating purinoceptors to increase Ca2+ influx, thus modulating microfilament organization, which, in turn, is essential for pollen germination and tube growth. Hence, we provide a mechanistic framework for the role played by eATP in pollen germination and tube growth.

Keywords

Picea meyeri Pollen germination Pollen tube growth eATP Gymnosperm 

Notes

Author contribution statement

Yanping Jing and Junhui Zhou designed the experiment, Junhui Zhou performed most of the experimental work, Kai Liu carried out ATP assay experiment, Junhui Zhou and Chengyu Fan wrote the paper. All authors read and approved the final manuscript.

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31371348), Program for Changjiang Scholars and Innovative Research Team in University (No. IRT13047).

Conflict of interest

We declare that we have no conflict of interest.

Supplementary material

468_2014_1135_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1340 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  • Junhui Zhou
    • 1
  • Chengyu Fan
    • 1
  • Kai Liu
    • 2
  • Yanping Jing
    • 1
  1. 1.College of Biological Sciences and TechnologyBeijing Forestry UniversityBeijingChina
  2. 2.Experimental Technology Center for Life SciencesBeijing Normal UniversityBeijingChina

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