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Development of SCAR marker for sex identification in dioecious Garcinia gummi-gutta

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We have developed sex-specific SCAR marker for the identification of dioecious Garcinia gummi - gutta (L.), which is useful for the selection of G. gummi - gutta at seedling stage and for plantation programmes.

Abstract

Garcinia gummi-gutta (L.) Robs. is a dioecious fruit yielding tree, which is naturally distributed as well as cultivated in the orchards in Western Ghat regions of India. A sex-linked DNA fragment was identified in Garcinia gummi-gutta (L.) Robs. by screening 150 randomly amplified polymorphic DNA primers and only one of them (OPBD20) showed different amplification band pattern associated with sex type. This sex-linked fragment was converted into male-specific sequence-characterized amplified region (SCAR) marker, CAM-566. The primers deigned in this study (OPBD20F and OPBD20R) correctly differentiated 12 male and 12 female plants at high annealing temperatures. Thus, a 556-bp band was amplified in male samples but not in female ones. Nevertheless, it should be noted that the fragments from both sexes were amplified at relatively low annealing temperatures. Additionally, the developed SCAR marker successfully identified the sexes of ten sex-unknown samples. Therefore, it can be used as an effective, convenient and reliable tool for sex determination in such dioecious species.

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Acknowledgments

The work was supported by the University Grants Commission, New Delhi [Project No. 41-423/2012 (SR)], Department of Biotechnology, New Delhi (DBT-KUD-IPLS Programme BT/PR14555/INF/22/126/2010), and Department of Atomic Energy, Mumbai (BRNS Project No. 2013/35/BRNS/20).

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Studies in Botany, Karnatak University, Dharwad, 580003, India

    Kadanthottu Sebastian Joseph & Hosakatte Niranjana Murthy

  2. Division of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore, 560089, India

    Kundapura Venkataramana Ravishankar

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  1. Kadanthottu Sebastian Joseph
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  2. Hosakatte Niranjana Murthy
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  3. Kundapura Venkataramana Ravishankar
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Correspondence to Hosakatte Niranjana Murthy.

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Communicated by F. Canovas.

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Joseph, K.S., Murthy, H.N. & Ravishankar, K.V. Development of SCAR marker for sex identification in dioecious Garcinia gummi-gutta . Trees 28, 1645–1651 (2014). https://doi.org/10.1007/s00468-014-1074-2

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