, Volume 27, Issue 4, pp 927–936 | Cite as

Climatic signals in tree-ring widths and wood structure of Pinus halepensis in contrasted environmental conditions

  • Klemen NovakEmail author
  • Martin de Luís
  • José Raventós
  • Katarina Čufar
Original Paper


Tree-ring widths (RW), earlywood (EW) and latewood (LW) widths, the transition from early to latewood (T) and the occurrence of intra-annual density fluctuations in EW (E-ring) and in LW (L-ring), as well as the presence of resin canals in EW and LW, were analyzed in Aleppo pine (Pinus halepensis Mill.) from three sites in Spain and one in Slovenia to find out if the anatomical characteristics can provide additional seasonal climate–growth information from contrasted environmental conditions. Principal component analysis was applied to elucidate the relationship between the measured parameters and climate. Principal component factor PC1 proved to be related to parameters of EW and the climatic variables of winter-spring; PC2 to parameters of LW and climatic variables of summer–autumn; PC3 to conditions during transitions from humid to dry periods. The three PCs vary between sites and are determined by the climatic conditions during their formation. The study demonstrates that wood anatomical features may provide complementary information to that contained in tree-ring widths. Since such results are obtained on contrasting sites, it is likely that it may be generalized over the wide range of P. halepensis distribution representing a useful proxy for studies on a regional scale.


Pinus halepensis Mediterranean Tree rings Intra-annual density fluctuations Anatomical characteristics 



This study was supported by Spanish Ministry of Education and Science, co-funded by FEDER Program (Project: CGL2008-05112-C02-01), and Slovenian Research Agency (Program P4-0015). We thank two anonymous referees for their comments as well as Elaine Rowe and Martin Cregeen for improving the English of this manuscript.


  1. Baillie MGL, Pilcher JR (1973) A simple cross-dating program for tree-ring research. Tree Ring Bull 33:7–14Google Scholar
  2. Barbéro M, Loisel R, Quezel P, Richardson MD, Romane F (1998) Pines of the mediterranean basin. In: Richardson DM (ed) Ecology and biogeography of Pinus. Cambridge University Press, Cambridge, pp 153–170Google Scholar
  3. Battipaglia G, de Micco V, Brand WA, Linke P, Aronne G, Saurer M, Cherubini P (2010) Variations of vessel diameter and δ13C in false rings of Arbutus unedo L. reflect different environmental conditions. New Phytol 188:1099–1112PubMedCrossRefGoogle Scholar
  4. Biondi F, Waikul K (2004) DENDROCLIM2002: a C ++ program for statistical calibration of climate signals in tree-ring chronologies. Comput Geosci 30:303–311CrossRefGoogle Scholar
  5. Bogino S, Bravo F (2009) Climate and intra-annual density fluctuations in Pinus pinaster subsp. mesogeenesis in Spanish woodlands. Can J For Res 39:1557–1565CrossRefGoogle Scholar
  6. Camarero JJ, Guerrero-Campo J, Gutierrez E (1998) Tree-ring growth and structure of Pinus uncinata and Pinus sylvestris in the Central Spanish Pyrenees. Arct Alp Res 30:1–10CrossRefGoogle Scholar
  7. Camarero JJ, Olano JM, Parras A (2010) Plastic bimodal xylogenesis in conifers from continental Mediterranean climates. New Phytol 185:471–480PubMedCrossRefGoogle Scholar
  8. Campelo F, Nabais C, Freitas H, Gutierrez E (2007) Climatic significance of tree-ring width and intra-annual density fluctuations in Pinus pinea from a dry Mediterranean area in Portugal. Ann For Sci 64:229–238CrossRefGoogle Scholar
  9. Campelo F, Nabais C, Gutierrez E, Freitas H, Garcia-Gonzalez I (2010) Vessel features of Quercus ilex L. growing under Mediterranean climate have a better climatic signal than tree-ring width. Trees Struct Funct 24:463–470CrossRefGoogle Scholar
  10. Cherubini P, Gartner BL, Tognetti R, Braker OU, Schoch W, Innes JL (2003) Identification, measurement and interpretation of tree rings in woody species from Mediterranean climates. Biol Rev 78:119–148PubMedCrossRefGoogle Scholar
  11. Cook E, Peters K (1997) Calculating unbiased tree-ring indices for the study of climatic and environmental change. Holocene 7(3):361–370CrossRefGoogle Scholar
  12. Cook E, Holmes R (1986) Users manual for Arstan program. Adapted from users manual for program ARSTAN. In: Holmes R, Adams RK, Fritts HC (eds) Tree-ring chronologies of Western North America: California, eastern Oregon and northern Great Basin. Laboratory of Tree-Ring Research, University of Arizona, Tucson, AZ, pp 50–65Google Scholar
  13. Copenheaver CA, Pokorski EA, Currie JE, Abrams MD (2006) Causation of false ring formation in Pinus banksiana: a comparison of age, canopy class, climate and growth rate. For Ecol Manage 236:348–355CrossRefGoogle Scholar
  14. de Luis M, Gričar J, Čufar K, Raventós J (2007) Seasonal dynamics of wood formation in Pinus halepensis from dry and semi-arid ecosystems in Spain. IAWA J 28:389–404Google Scholar
  15. de Luis M, Novak K, Raventós J, Gričar J, Prislan P, Čufar K (2011a) Climate factors promoting intra-annual density fluctuations in Aleppo pine (Pinus halepensis) from semiarid sites. Dendrochron 29:163–169CrossRefGoogle Scholar
  16. de Luis M, Novak K, Raventós J, Gričar J, Prislan P, Čufar K (2011b) Cambial activity, wood formation and sapling survival of Pinus halepensis exposed to different irrigation regimes. For Ecol Manage 262:1630–1638CrossRefGoogle Scholar
  17. de Micco V, Aronne G, Baas P (2008) Wood anatomy and hydraulic architecture of stems and twigs of some Mediterranean trees and shrubs along a mesic-xeric gradient. Trees Struct Funct 22:643–655CrossRefGoogle Scholar
  18. Fonti P, von Arx G, García-González I, Eilmann B, Sass-Klasen U, Gärtner H, Eckstein D (2010) Studying global change through investigation of the plastic responses of xylem anatomy in tree rings. New Phytol 185:42–53PubMedCrossRefGoogle Scholar
  19. Froux F, Huc R, Ducrey M, Dreyer E (2002) Xylem hydraulic efficiency versus vulnerability in seedlings of four contrasting Mediterranean tree species (Cedrus atlantica, Cupressus sempervirens, Pinus halepensis and Pinus nigra). Ann For Sci 59:409–418CrossRefGoogle Scholar
  20. Hoffer M, Tardif J (2009) False rings in jack pine and black spruce trees from eastern Manitoba as indicators of dry summers. Can J of For Res 39:1722–1736CrossRefGoogle Scholar
  21. Hoffmann WA, Marchin REM, Abit P, Lau OL (2011) Hydraulic failure and tree dieback are associated with high wood density in a temperate forest under extreme drought. Glob Change Biol 17:2731–2742CrossRefGoogle Scholar
  22. Holmes R (1994) Dendrochronology program library user’s manual. Laboratory of Tree-Ring Research, University of Arizona, Tucson, USAGoogle Scholar
  23. Kaiser HF (1992) On Cliff’s formula, the Kaiser–Guttman rule, and the number of factors. Percept Mot Skills 74:595–598CrossRefGoogle Scholar
  24. Lebourgeois F, Rathgeber C, Ulrich E (2010) Sensitivity of French temperate coniferous forest to climate variability and extreme events (Abies alba, Picea abies and Pinus sylvestris). J Veg Sci 21:364–376CrossRefGoogle Scholar
  25. Martinez-Meier A, Sanchez L, Pastorino M, Gallo L, Rozenberg P (2008) What is hot in tree rings? The wood density of surviving Douglas-firs to the 2003 drought and heat wave. For Ecol Manage 256:837–843CrossRefGoogle Scholar
  26. McCarroll D, Jalkanen R, Hicks S, Tuovinen M, Gagen M, Pawellek F, Dieter E, Schmitt U, Autio J, Heikkinen O (2003) Multiproxy dendroclimatology: a pilot study in northern Finland. Holocaust 13:899–900Google Scholar
  27. Novak K, de Luis M, Čufar K, Raventós J (2011) Frequency and variability of missing tree rings along the stems of Pinus halepensis and Pinus pinea from a semiarid site in SE Spain. J Arid Environ 75:494–498CrossRefGoogle Scholar
  28. Olivar J, Bogino S, Spiecker H, Bravo F (2012) Climate impact on growth dynamics and intra-annual density fluctuations in Aleppo pine (Pinus halepensis) tree on different crown clases. Dendrochronologia 30:35–47CrossRefGoogle Scholar
  29. Osborn TJ, Briffa KR, Jones PD (1997) Adjusting variance for sample-size in tree-ring chronologies and other regional mean time series. Dendrochronologia 15:89–99Google Scholar
  30. Rathgeber C, Misson L, Nicault A, Guiot J (2005) Bioclimatic model of tree radial growth: application to French Mediterranean Allepo pine forest. Trees 19:162–176CrossRefGoogle Scholar
  31. Richardson DM, Rundel PW (1998) Ecology and biogeography of Pinus: an introduction. In: Richardson DM (ed) Ecology and biogeography of Pinus. Cambridge University Press, Cambridge, UK, pp 3–46Google Scholar
  32. Rigling A, Brühlhart H, Bräker OU, Forster T, Schweingruber FH (2003) Effect of irrigation on diameter growth and vertical resin duct production in Pinus sylvestris L. on dry sites in the central Alps, Switzerland. For Ecol Manage 175:285–296CrossRefGoogle Scholar
  33. Rozas V, Garcia-Gonzalez I, Zas R (2011) Climatic control of intra-annual wood density fluctuations in Pinus pinaster in NW Spain. Trees Struct Funct 25:443–453CrossRefGoogle Scholar
  34. Schweingruber FH (1988) Tree rings, basics and application of dendrochronology. Kluwer Academic Publishers, Dorecht, BostonCrossRefGoogle Scholar
  35. Vieira J, Campelo F, Nabais C (2009) Age-dependent responses of tree-ring growth and intra-annual density fluctuations of Pinus pinaster to Mediterranean climate. Trees Struct Funct 23:257–265CrossRefGoogle Scholar
  36. Wigley TM, Briffa KR, Jones PD (1984) On the average value of correlated time-series, with applications in dendroclimatology and hydrometeorology. J Clim Appl Meteor 23:201–213CrossRefGoogle Scholar
  37. Wimmer R, Grabner M (1997) Effects of climate on vertical resin duct density and radial growth of Norway spruce (Picea abies (L) Karst). Trees Struct Funct 11:271–276Google Scholar
  38. Wimmer R, Strumia G, Holawe F (2000) Use of false rings in Austrian pine to reconstruct early growing season precipitation. Can J For Res 30:1691–1697CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Klemen Novak
    • 1
    • 2
    • 3
    Email author
  • Martin de Luís
    • 1
  • José Raventós
    • 2
  • Katarina Čufar
    • 3
  1. 1.Department of GeographyUniversity of ZaragozaZaragozaSpain
  2. 2.Department of EcologyUniversity of AlicanteSan Vicente del Raspeig, AlicanteSpain
  3. 3.Department of Wood Science and Technology, Biotechnical FacultyUniversity of LjubljanaLjubljanaSlovenia

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