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Proteomic and physiological analyses reveal detoxification and antioxidation induced by Cd stress in Kandelia candel roots

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Abstract

The heavy metal Cadmium (Cd), added to the water bodies through weathering of rocks and human activities, constitutes one of the major environmental pollutants toxic to plants. This study examines the proteome changes in roots of actively growing Kandelia candel (L.) Druce when challenged with Cd. This mangrove-like species proliferates in estuaries and bays and is a potential choice for phytoremediation of Cd. A total of 53 proteins were up- or down-regulated following a short-term Cd treatment. The identities of the differentially expressed proteins were determined by MALDI-TOF/TOF. Approximately half of the up-regulated proteins are involved in oxidative response, including antioxidant enzymes, enzymes required for glutathione biosynthesis, enzymes in TCA and PPP cycles for generating ATP, NADH and NADPH. These results support the prediction that a prompt antioxidative response is necessary for the reduction of the oxidative stress caused by Cd and set the stage for further investigating of Cd up-regulated proteins in Kandelia candel.

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Acknowledgments

This work was supported by the National Natural Science Grant of China (Award No. 31070542) and Natural Science Foundation of Fujian Province, China (Award No. 2010J01067). We also would like to thank Dr. Erin Irish of the Department of Biology at The University of Iowa (USA) for the critical reviewing of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Corps, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Zhao-Xia Weng, Ling-Xia Wang, Jian-Hong Xing & Wei Chen

  2. School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Zhao-Xia Weng, Ling-Xia Wang, Jian-Hong Xing & Wei Chen

  3. Fujian Academy of Forestry, Fuzhou, 350012, China

    Fang-lin Tan & Li Huang

  4. College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Shi-Pin Chen

  5. Department of Biology, The University of Iowa, 210 Biology Building, Iowa City, IA, 52242, USA

    Chi-Lien Cheng

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  1. Zhao-Xia Weng
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Correspondence to Wei Chen.

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Communicated by H. Rennenberg.

Z.-X. Weng and L.-X. Wang contributed equally.

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Weng, ZX., Wang, LX., Tan, Fl. et al. Proteomic and physiological analyses reveal detoxification and antioxidation induced by Cd stress in Kandelia candel roots. Trees 27, 583–595 (2013). https://doi.org/10.1007/s00468-012-0811-7

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