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Genetic diversity and genetic structure of different populations of the endangered species Davidia involucrata in China detected by inter-simple sequence repeat analysis

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Abstract

Dove tree (Davidia involucrate) is a Tertiary relic species endemic to China and is reputed to be a ‘living fossil’ in the plant kingdom. Genetic diversity and genetic structure of this species were analyzed for its conservation and management, using inter-simple sequence repeat (ISSR) data obtained from eight populations distributed throughout seven provinces of China. A relatively high level of genetic diversity, at both population and species levels, was detected using the POPGENE software. Analysis of molecular variance (AMOVA) revealed a moderate level of among-population variation (i.e., 33.21%). The genetic structure of dove tree was closely consistent with their isolated topographical distribution region based on the results of the STRUCTURE, POPGENE-UPGMA and PCA analysis. It is postulated that the relatively high level of genetic diversity has been maintained because of: (a) the original wild geographical distribution, (b) propagation through outcrossing seeds or root suckers, (c) the longevity of individuals and (d) the relatively little human disturbance. Genetic drift and restricted gene are important factors affecting genetic differentiation. There was no significant correlation between geographical distances and a pairwise comparison with genetic distances, as analyzed by the Mantel test, but the clustering result of genetic diversity was consistent with their isolated topographical distribution regions. Thus, maintaining the stable special habitats associated with this species is recommended for the in situ conservation. Furthermore, it is important to develop an effective seed germination system for the maintenance of an ex situ conservation pool of the germplasm resources.

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Acknowledgments

This research was supported by grants from Botanic Gardens Conservation International (6206/R4361) and the Natural Science Foundation of Hubei Province (2006ABA035). We thank all past and present colleagues in our labotatory for constructive discussion and technical support, and Dr. Alex McCormac (Mambo-Tox Ltd., UK) for critical editing of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Shijia Luo, Yanhong He, Guogui Ning, Jiaqi Zhang, Guangying Ma & Manzhu Bao

  2. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, Enshi, 445000, People’s Republic of China

    Shijia Luo

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  1. Shijia Luo
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  2. Yanhong He
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  3. Guogui Ning
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  4. Jiaqi Zhang
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  5. Guangying Ma
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  6. Manzhu Bao
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Correspondence to Manzhu Bao.

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Communicated by F. Canovas.

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Luo, S., He, Y., Ning, G. et al. Genetic diversity and genetic structure of different populations of the endangered species Davidia involucrata in China detected by inter-simple sequence repeat analysis. Trees 25, 1063–1071 (2011). https://doi.org/10.1007/s00468-011-0581-7

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  • DOI: https://doi.org/10.1007/s00468-011-0581-7

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