Relationships of stable carbon isotopes, plant water potential and growth: an approach to asses water use efficiency and growth strategies of dry land agroforestry species

Abstract

The relationships between annual wood stable carbon isotope composition (δ13C), dry season midday plant water potential, and annual growth rate were investigated to asses the ability of agroforestry species to adapt to climate changes. 6–8 stem disks from four co-occurring species (Acacia senegal, A. seyal, A. tortilis and Balanites aegyptiaca) were collected for radial growth measurements using tree-ring analysis spanning 1930–2003. Annual δ13C was measured on three tree disks per species for the period 1970–2002. Midday plant water potential was measured during the dry season. Annual radial growth and midday plant water potential ranged from 0.27 to 9.12 mm and −1.0 to −5.0 MPa, respectively, with statistically significant differences. After correcting annual wood δ13C for atmospheric changes in δ13C, carbon isotopic composition ranged from −22.22 to −26.58‰. Relationships between δ13C, radial growth and plant water potentials revealed the interaction of water availability, stomatal conductance, δ13C values and growth. Two contrasting water use strategies and competitive advantages can be distinguished. Species with lower mean δ13C values (A. senegal and A. seyal) show high plant water potential and, hence, better growth during moist years. Thus, they indicate low water use efficiency (WUE) and opportunistic water use strategy. On the other hand, species with lower water potentials (A. tortilis and B. aegyptiaca) showed relative better growth performance and less increase in δ13C in drought years, reflecting their high WUE and conservative water use strategy. These results suggest that δ13C in tree rings can be useful in estimating historic changes in plant WUE and hence in screening drought tolerant species in the face of expected climate changes, as well as for assessing the functional diversity and risk reduction in mixed vegetation.

Introduction

Global climate change is expected to modify patterns of rainfall variability and the composition of vegetation responds to that, especially in dry regions of Africa (IPCC 2007). Assessment of vegetation level vulnerability, climate change resilience and human adaptation options requires understanding of the diversity, among tree species in the current vegetation, of genetic and phenotypic growth strategies and response to fluctuating water availability (Dawson et al. 2009). Human livelihoods in many parts of Africa depend on the use and management of trees in forms of dryland agroforestry (Poschen 1986). Acacias and close relatives are important agroforestry species and major components of the vegetation in arid and semi-arid parts of Africa. There is a wide range of life history and phenology traits in this group, hence tree water use and water use efficiency (WUE) must also be determined to support decision-making in tree domestication and reforestation efforts. For instance, under drought prone conditions, species with a wide capacity to withstand hydrological changes may be an advantage while species with better growth performance could be suitable in areas of non-limiting water conditions. This is imperative especially in countries such as Ethiopia where previous reforestation programs, attempted to counteract the effects of deforestation, have failed mainly due to lack of knowledge on the ecology and adaptation to environmental stress (Gebrekirstos 2006).

An important physiological trait related to water use and stomatal behavior under drought conditions is the ratio of internal to ambient CO2 concentration (C i/C a) of leaves (Farquhar et al. 1989). The stomata are not only the gates for carbon dioxide diffusion into the intracellular leaf space but also control water loss due to water vapor diffusion out of the leaf (transpiration). A number of environmental factors exert stress on plants, which can affect the rate of photosynthesis or stomatal conductance, and thus influence C i and carbon isotope ratio (δ13C) in plant matter. Therefore, growth, plant water potential and stable carbon isotopes were considered simultaneously as a measure of stress tolerance. The carbon isotope ratio (δ13C) of plants is isotopically depleted in 13C with respect to atmospheric CO2 (Farquhar et al. 1989). According to Farquhar et al. (1982), isotopic composition is a function of isotopic fractionation associated with differential diffusivities of 13CO2 versus 12CO2 in the air and stomata (a = 4.4‰) and the net fractionation by Rubisco during the photosynthetic CO2 fixation reaction (b = 27‰), the ratio of the intercellular (C i) to atmospheric CO2 (C a) concentration (C i/C a) and the current δ13C value of the atmosphere (−8‰) expressed as:

$$ \delta^{ 1 3} {\text{C}} = \delta^{ 1 3} {\text{C}}_{\text{atm}} - a - \left( {b - a} \right) \, \left( {C_{\text{i}} /C_{\text{a}} } \right). $$

A broad relationship exists between carbon isotope composition of plant dry matter and the efficiency with which this dry matter is produced relative to the amounts of water which the plant transpires (Farquhar et al. 1989). A significant positive correlation has been found between δ13C and WUE both theoretically and empirically (Farquhar et al. 1982, 1989). Net instantaneous WUE, the ratio of carbon assimilation to evapo-transpiration, has been found to co-vary with δ13C in a sense that high δ13C values correspond to high WUE and vice versa (Farquhar et al. 1988). Plants can achieve high WUE through either low transpiration, or high net photosynthesis, or both. Since both water and CO2 diffuse through the stomata, traits that determine isotopic composition of plant tissues have important implications for transpiration, productivity and plant survival in water limited environments (Ehleringer 1993).

Plant water potential also plays a key role in connecting water uptake, leaf hydration status (Vertovec et al. 2001) stomatal closure, opportunities for cell wall expansion (Macfarlane and Adams 1998) and the total use and allocation of photosynthate for growth of various organs (Breda et al. 1993). More drought-resistant species tend to reach low water potentials more rapidly than less resistant species (Ladiges 1975). On the basis of the diurnal range in plant water potential (predawn, midday), Gebrekirstos et al. (2006) classified Acacia senegal and A. seyal into drought avoiders and A. tortilis and Balanites aegyptiaca as drought tolerant in Ethiopia. The latter two species had lower (more negative) midday plant water potential. In addition to plant water potential, however, study of interannual variation through tree ring growth and isotope composition adds a further dimension and allows to scale up to the life time of trees (Gebrekirstos 2006). The tradeoffs between avoidance and tolerance at interannual scales can be linked to their WUE across the range of climatic conditions that may have occurred over the life time of the tree. Hence, to further explore the water use strategy of the four tree species studied by Gebrekirstos et al. (2006), we synthesized three approaches that provide different information about drought response: (a) midday plant water potential values during the dry season, as a measure of plant response to water stress (as described by Gebrekirstos et al. 2006), (b) radial growth spanning 1930–2002 as a measure of growth response under changing water availability (as described by Gebrekirstos et al. 2008), (c) δ13C in tree rings for the period 1970–2002 as a measure of change in WUE in response to rainfall amount. Our specific objectives were (a) to determine if correlations exist between carbon isotope ratios, plant water potential and radial growth, (b) to use these relationships to characterize species-specific water use strategy and tolerance to moisture stress in the face of climate change.

Materials and methods

Study area

The study area, Abernosa ranch, is located within the upper Rift Valley Lakes Sub region, 170 km south of Addis Ababa, 7°51′N to 38°42′E at an elevation of about 1,600 m. The area is classified as semi-arid eco-climatic vegetation zone (Makin et al. 1975) and is a major part of the country’s Acacia woodland and savanna region.

The climate of the area is characterized by a distinct dry season. The long and short rainy periods occur from July to September and April to June, respectively. The wettest month is July with an average precipitation of 142 mm. Average annual precipitation, measured at Adami Tulu Research Center Meteorological Station, amounts to 760 mm and ranges between 550 and 900 mm (Fig. 1). Mean annual temperature is 20.7°C and varies little from year to year. The soils are sandy loam with pH of 6.75.

Fig. 1
figure1

Climatic diagram of the study sites (in Abernosa) drawn according to Walter (1973). Rainfall and temperature data (1980–2002) were obtained from Adamitulu Research Meteorological Station, which is situated at about 5 km from Abernosa

Study species descriptions

The study species are Acacia tortilis (Forssk.) Hayne, Acacia seyal Del, Acacia senegal (L.) Wild., and Balanites aegyptiaca (L.) Del. The Acacias are drought deciduous and are members of Fabaceae family, while the evergreen Balanites aegyptiaca belongs to Balanitaceae. Acacias provide important ecological and economical services for local communities all over Africa. The habitat/ecology, geographical distribution and uses of the study species are reported in detail elsewhere (Azene-Bekele et al. 1993; Hedberg and Edwards 1989; van Noordwijk 1984).

According to long-term climate–growth analysis of the species by Gebrekirstos et al. (2008), tree ring width varied considerably (0.27–9.12 mm) between years and among the species. Higher growth rates in all the species were related to moist years (Table 1). A study on plant water potential parameters, using a pressure chamber (Scholander et al. 1965), at sites with different biophysical settings and diurnal differences also revealed significant differences among the species (Gebrekirstos et al. 2006). The habit, midday plant water potentials and annual radial growth characteristics are summarized in Table 1.

Table 1 List of studied species with summary of their habit, midday plant water potential values and annual growth increments

Stable carbon isotopes

Three stem disks per species were selected from the tree disks that had been used for the tree-ring analysis. Individual δ13C series measurements were performed on bulk wood with annual resolution for the years 1973–2002. The stable carbon isotopic signatures of the wood samples were measured online using an NA 2500 elemental analyzer (CE Instruments, Rodano, Italy), linked with an isotope ratio mass spectrometer (Finnigan MAT Delta plus, Bremen, Germany) via a ConFlo III interface at the Center for Stable Isotope Research and Analysis, Forest Ecosystem Research, University of Göttingen, Germany. The internal reproducibility of the mass spectrometer was 0.1–0.15‰. The standard deviation of δ13C for the repeated analysis of NBS 21 (Graphite) was 0.07‰. Variations of isotope ratios are expressed in δ-notation, i.e. the relative deviation from the PDB standard and given as: δ 13C = [(13C/12C) sample/(13C/12C) PDB) − 1] × 1,000‰.

Data analysis

Tree-ring isotopic data contain trends and variability unrelated to past climate. One non-climatic trend that can be removed from δ13C series is related to the decline in atmospheric δ13C values caused by burning of fossil fuels. This trend was removed following the method described in McCarroll and Loader (2004). However, the strong declining trend on series of B. aegyptiaca after 1992 remains in our records even after correcting for change in δ13C air (Fig. 2). The δ13C values were averaged into mean, maximum and minimum values. According to rainy season precipitation amount, the δ13C values (1973–2002) were allocated into drought (11 years) and moist years (19 years).

Fig. 2
figure2

a Rainy season precipitation indices (June–September), b ring width indices (each of the species curves represents the mean of six trees), c inter-annual pattern of δ13C signature measured on bulk wood of four co-occurring tree species (each of the species curves represents the mean of three trees)

To determine the relationships between radial growth, plant water potential and δ13C variations, we considered δ13C (mean, maximum, minimum, and mean values for drought and moist years), ring width (mean, minimum, and maximum) and midday plant water potential values. The values for the last two parameters (Table 1) are refereed from our past research on plant water potential (Gebrekirstos et al. 2006) and climate growth relationships (Gebrekirstos et al. 2008). δ13C comparisons among the species were determined using one-way analysis of variance (ANOVA). STATISTICA for windows (Version 6.0) was used as a tool for the data analyses. Unless stated otherwise, results are statistically significant at P < 0.05.

Results

Species-specific δ13C values

Mean δ13C values of the study species ranged from −24.31 to −24.76‰, a total range of 0.45‰. Given a sample size of three, the standard error values obtained from all species falls between 0.08 and 0.38 (Table 2), indicating that the δ13C signature of individual trees remained close to the mean values. A. senegal had lower (more negative) mean and minimum δ13C values (−24.76 ± 0.08, −26.28 ± 0.26‰) followed by A. seyal (−24.62 ± 0.34, −25.95 ± 0.53‰). Mean, maximum and minimum δ13C values of A. senegal, A. seyal and B. aegyptiaca were not significantly different from each other. A. senegal showed a significant lower mean and minimum δ13C values than A. tortilis. A. tortilis showed no significant differences either with A. seyal or B. aegyptiaca. Though A. senegal and A. seyal showed the highest (less negative) maximum values, they showed no significant difference with B. aegyptiaca and A. tortilis. However, unlike the Acacias, B. aegyptiaca has showed a declining trend starting 1992 (Fig. 2c), and this has contributed to lower (more negative) values in the mean, minimum and maximum δ13C. Excluding the 1991–2002 data resulted in less negative values (Table 2, values in brackets) compared to those of the Acacias.

Table 2 δ13C mean, maximum and minimum values of the study species (number of samples was 3)

Inter-annual variation (difference between maximum and minimum δ13C values) ranged from −22.22 to −26.58‰, a total range of 3.8‰, with both extremes occurring in A. seyal and A. senegal, respectively. The highest range was that of A. seyal (2.37–3.71‰) followed by A. senegal (3.18–3.39‰), while A. tortilis showed the lowest range (1.3–1.8‰). On the other hand, inter-annual variation in B. aegyptiaca prior and after the declining trend in δ13C ranges from 1 to 3.48‰, respectively (Fig. 2c).

δ13C values in drought and moist years

Although the studied species revealed little differences in average mean values, they showed clear differences in drought and normal (moist) years (Fig. 2; Table 3). In all species, mean δ13C values in normal (moist years) were lower (more negative) than in drought years. Especially, A. senegal and A. seyal showed distinct variations between moist and dry years as compared with A. tortilis and B. aegyptiaca. B. aegyptiaca showed no significant difference between drought and moist years (Table 3).

Table 3 Two-sample tests of δ13C values of the species compared between drought years and other years for the period of 1973–2002

Relationships between δ13C, radial growth and plant water potential

The δ13C values and the respective ring width variations of all species showed negative correlations. Enriched δ13C values were found in narrow rings (drought years) compared to more depleted δ13C values in wide rings (moist years) (Table 3; Fig. 2).

Mean, minimum and maximum δ13C values of all the species were negatively correlated with mean, maximum and minimum radial growth, respectively (Fig. 3). In contrast, midday plant water potential showed positive correlation (r = 0.88) with mean and maximum radial growth but negative correlation with minimum radial growth (Fig. 4). Even more remarkable is the observed tendency for species having lower mean δ13C values to show high plant water potential (Figs. 3, 4) and, hence, better growth during moist years (Fig. 2). On the other hand, in drought years those species with lower water potentials (A. tortilis and B. aegyptiaca) showed better growth performances and lower mean δ13C values.

Fig. 3
figure3

Correlation coefficient of maximum (A), mean (B) and minimum growth rates of each species (C): filled circle (A. senegal), filled triangle (A. seyal), filled diamond (A. tortilis), filled square (B. aegyptiaca) with minimum, mean and maximum δ13C values of each species, respectively

Fig. 4
figure4

Correlation coefficient of maximum (A), mean (B) and minimum growth rates of each species (C): filled circle (A. senegal), filled triangle (A. seyal), filled diamond (A. tortilis), filled square (B. aegyptiaca) with midday plant water potential of each species (r = 0.88, 0.72 and −0.93, respectively)

Discussion

After correcting annual wood tissue δ13C for atmospheric changes in δ13C, carbon isotopic composition ranged from −26.58 to −22.22‰, which falls within the range observed in C3 plants (−32 to −20‰) (Ehleringer 1993). However, the range of mean values (<1‰) obtained within and among the investigated species is very small compared to similar studies elsewhere. For instance, Ramesh et al. (1985) found differences of ca. 3‰ between four conifer species in the Kashmir Valley of India and Hemming et al. (1998) found a difference of ca. 2.5‰ in Eastern England. McCarroll and Pawellek (1998) found a range of 2.15‰ within Pinus sylvestris. Others have reported that differences of individuals within a species, even growing at the same site, can be as large as differences found between species (Leavitt and Long 1984; Leavitt and Lara 1994; Brendel et al. 2002). Although not statistically significant, mean values of A. s enegal and A. seyal show lower (more negative) δ13C values compared to A. tortilis and B. aegyptiaca. This can be attributed to their water use strategy, which will be discussed below.

The higher interannual fluctuation in δ13C values in A. senegal and A. seyal compared to A. tortilis and B. aegyptiaca (Fig. 2) may indicate that A. senegal and A. seyal did not maintain a consistent stomatal response under conditions of changing water availability. This is reflected by higher (more negative) δ13C values in moist years (Table 3) which may reflect improved stomatal conductance. However, A. tortilis and B. aegyptiaca showed a lower decrease in δ13C values during moist years, indicating that stomatal regulation changed little between moist and dry years. This may be understood as a regulative adaptation of the trees to moisture stress in a way that uses constant stomatal closure as a set point for gas exchange. The lower midday water potential values of A. tortilis and B. aegyptiaca also support this interpretation.

In general, evergreen leaves tend to have lower stomatal conductance than deciduous leaves (Sobrado 1986, 1991). The lack of significant difference in δ13C values of B. aegyptiaca (evergreen species) in drought and moist years supports this general trend and thus it may be considered as an adaptive strategy. In contrast to the deciduous Acacias, however, the values of B. aegyptica showed a declining trend of δ13C after 1991 (Fig. 2) which is not accompanied by radial growth increments regardless of precipitation amounts. Excluding the 1991–2002 data, in the absence of clear understanding of its cause except assumptions (Gebrekirstos et al. 2009), resulted in higher δ13C values (less negative) compared to those of the Acacias and it fits to the general observation.

The underlying process which controls the δ13C ratio in C3 plants is well understood (Francey and Farquhar 1982; Farquhar et al. 1989). Photosynthetic fractionation of 13C might explain the negative correlations found between δ13C and ring width and precipitation amount in this study. In drought years, the stomata will be more closed and hence the C i/C a ratio will be relatively low because the CO2 supply through the stomata is limited. However, in moist years, when drought stress is reduced, growth may increase along with increased stomatal conductance, resulting in wider rings and lower (more negative) δ13C. Such a negative correlation between δ13C and ring width has been reported elsewhere (Tans and Mook 1980). Consistent with our reasoning, Treydte et al. (2001) found increasing carbon isotope ratios due to low air humidity or low soil moisture as a result of reduced stomatal conductance. In contrast, Saurer et al. (1997) reported a lack of correlation between ring width and δ13C. They further commented that causal links should not be made between carbon isotope and ring width since the plant may vary stomatal conductance and photosynthetic capacity in common and C i may be kept constant. This could be so as their tree-ring width variations were influenced by local growth conditions.

The observed relationships between δ13C, radial growth and plant water potentials, allow further interpretation of the continuum between the two contrasting water use strategies and its consequences for competitive advantage and vegetation level complementarity. From our results it is apparent that A. senegal and A. seyal with the higher water potential values (−1.55 to −2.68 MPa) tend to have low δ13C values (up to −26.58‰) as well as better growth (up to 9.12 mm) during moist years. During drought years, when soil water becomes very limiting, those species showed an enormous reduction in growth (up to 0.27 mm) and increases in δ13C values (up to −22.2‰). A. tortilis and B. aegyptiaca that exhibited low midday water potential values (−3.05 to −4.61 MPa) showed significantly lower maximum growth during moist years (up to 5.25 mm) but were able to maintain a relatively higher growth (0.34 mm) and showed smaller fluctuations in δ13C values in drought years (Table 3). In fact, δ13C values of B. aegyptiaca were lowered to below −26‰ as a result of the declining trend but this was not accompanied by growth increase.

These differences between trees fit the pattern described by Ehleringer and Cooper (1988) of a negative correlation of potential growth rate and WUE when soil resources are not limited. Chapin (1991) has proposed that an essential trait of plants adapted to low resource environments is that they grow slowly even when an optimal supply of resources is provided.

Orwig and Abrams (1997), in their studies of radial growth responses to drought in the tree-ring records of six species growing within two locations of differing land-use history, canopy position and soil moisture characteristics, noted that during moist years, drought-sensitive species show enhanced growth than drought-tolerant species, while they show more reduced growth during drought years. A lower increase in WUE (δ13C values) under drier conditions by drought tolerant (Quercus ilex) compared to drought sensitive (Pinus halepensis) species has also been reported by Ferrio et al. (2003).

In a mixed vegetation, A. seyal and A. senegal would be favored during moist years because of their capacity for more rapid growth under wet conditions. On the other hand, A. tortilis and B. aegyptiaca have an advantage over A. seyal and A. senegal during dry years or dry environment as a consequence of a greater capacity for water conservation. Raddad and Lukkanen (2006) examined genetic variation in WUE and gum yield in A. senegal provenances grown on sandy and clay soil areas in Sudan. The clay provenances show more negative δ13C and higher growth rates indicating less conservative water use compared to sandy soil provenance. For dioecious trees the strategies may differ between male and female trees. Ward et al. (2002) reported that female Acer negundo trees show higher growth rates and lower isotope ratios in wet years, which imply that they are less conservative water users compared to male Acer negundo trees. However, alpha cellulose measurements of female and male Acer negundo trees did not differ during dry years, indicating that, on average, both maintained similar stomatal conductance under dry conditions.

Conclusions

Our study revealed that the δ13C of annual growth rings was negatively correlated with average annual radial growth (r = up to −0.77). Mean and maximum radial growth and plant water potential were positively correlated (r = 0.87), but negatively correlated with minimum growth. Remarkably, species with lower mean δ13C values tend to show high plant water potential and, hence, better growth during moist years. On the other hand, in drought years those species with lower water potentials showed relative better growth performances (reflected by negative correlation with minimum growth) and less increase in δ13C values. Consequently, A. senegal and A. seyal are characterized as low WUE and opportunistic water users while A. tortilis and B. aegyptiaca are high WUE and conservative water users. From our results it is evident that δ13C signatures in tree rings are able to reflect life time reaction of the tree to water availability changes and, therefore, δ13C in tree rings can be useful to screen species for agroforestry and in restoration/reforestation of degraded lands. However, in order to use δ13C effectively in a program to select trees for stressful site conditions, we recommend applying an integrated approach. Based on these findings, if survival under drought and maintaining acceptable growth under water limited conditions are both critical, species with high δ13C values (high WUE plants) or species with low water potential values would have a better survival and increment than low WUE plants. Therefore, they would be more suitable for planting in arid areas. Under conditions where water is limiting, but relatively high soil moisture is available, and of course under non-limiting water conditions, species with low WUE are better competitors.

We recommend that future studies investigate whether the relationships and differences between the species observed in this study are also pertinent in other environmental settings.

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Acknowledgments

The study was financed by the German Academic Exchange Service (DAAD), which is gratefully acknowledged. We thank Wondo Genet College of Forestry, Hawassa University for logistic support. We extend our thank to the staff members of Abernosa Ranch for their kind assistance and logistic support during the fieldwork.

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Correspondence to Aster Gebrekirstos.

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Contribution to the special issue “Tropical Dendroecology”.

Communicated by A. Bräuning.

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Gebrekirstos, A., van Noordwijk, M., Neufeldt, H. et al. Relationships of stable carbon isotopes, plant water potential and growth: an approach to asses water use efficiency and growth strategies of dry land agroforestry species. Trees 25, 95–102 (2011). https://doi.org/10.1007/s00468-010-0467-0

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Keywords

  • Climate change
  • Dendrochronology
  • Drought tolerance
  • Ethiopia
  • Water use efficiency
  • Tree rings