Sprouting axillary buds sampled from a mature 27-year-old shrub of Cornus mas ‘Macrocarpa’ were used as starting material for in vitro culture establishment. Multiple shoot cultures, grown on basal woody plant medium with the pH adjusted to 5.6–5.7 and supplemented with 6-benzylaminopurine in combination with 1-naphthaleneacetic acid, were capable of continuous axillary and adventitious shoot proliferation up to 1 year. Later on, growth ceased, shoot tip necrosis appeared and shoot cultures died. Transfer of living shoots onto modified woody plant medium with the pH adjusted to 6.8–7.0 led to vigorous growth of multiple shoot cultures without any loss of multiplication rates or decreased vitality for several years. The use of 6-benzylaminopurine in combination with 1-naphthaleneacetic acid proved superior to the application of thidiazuron which induced a frequent formation of short and fasciated shoots. 1-naphthaleneacetic acid promoted in vitro adventitious rooting frequency up to 73.3%, whereas indole-3-butyric acid was not effective. Ex vitro acclimatized plants did not show any visually detectable morphological variation.
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The author thanks Dr. D. Gömöry for statistical advice, Mrs. H. Parobková for preparation of culture media, and Mrs. A. Lengyelová for excellent care for plantlets transferred to ex vitro environment. This work was financed by the Slovak Grant Agency VEGA (1/3262/06 and 1/3514/06).
Communicated by D. Treutter.
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Ďurkovič, J. Micropropagation of mature Cornus mas ‘Macrocarpa’. Trees 22, 597–602 (2008). https://doi.org/10.1007/s00468-008-0228-5