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Micropropagation of mature Cornus mas ‘Macrocarpa’


Sprouting axillary buds sampled from a mature 27-year-old shrub of Cornus mas ‘Macrocarpa’ were used as starting material for in vitro culture establishment. Multiple shoot cultures, grown on basal woody plant medium with the pH adjusted to 5.6–5.7 and supplemented with 6-benzylaminopurine in combination with 1-naphthaleneacetic acid, were capable of continuous axillary and adventitious shoot proliferation up to 1 year. Later on, growth ceased, shoot tip necrosis appeared and shoot cultures died. Transfer of living shoots onto modified woody plant medium with the pH adjusted to 6.8–7.0 led to vigorous growth of multiple shoot cultures without any loss of multiplication rates or decreased vitality for several years. The use of 6-benzylaminopurine in combination with 1-naphthaleneacetic acid proved superior to the application of thidiazuron which induced a frequent formation of short and fasciated shoots. 1-naphthaleneacetic acid promoted in vitro adventitious rooting frequency up to 73.3%, whereas indole-3-butyric acid was not effective. Ex vitro acclimatized plants did not show any visually detectable morphological variation.

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  • Declerck V, Korban SS (1994) Effects of source of macronutrients and plant growth regulator concentrations on shoot proliferation of Cornus florida. Plant Cell Tissue Organ Cult 38:57–60

    Article  CAS  Google Scholar 

  • Edson JL, Wenny DL, Leege-Brusven A (1994) Micropropagation of Pacific dogwood. HortScience 29:1355–1356

    Google Scholar 

  • Hadziabdic D, Trigiano RN, Garton S, Windham MT (2004) In vitro regeneration of Cornus kousa. S Nurs Assoc Res Conf Proc 49:356–358

    Google Scholar 

  • Kaveriappa KM, Phillips LM, Trigiano RN (1997) Micropropagation of flowering dogwood (Cornus florida) from seedlings. Plant Cell Rep 16:485–489

    CAS  Google Scholar 

  • Lloyd GB, McCown BH (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Proc Int Plant Prop Soc 30:421–427

    Google Scholar 

  • Pennell D (1983) The future use of micropropagation in the United Kingdom. Proc Int Plant Prop Soc 33:249–253

    Google Scholar 

  • Preece JE, Huetteman CA, Ashby WC, Roth PL (1991) Micro- and cutting propagation of silver maple. 1. Results with adult and juvenile propagules. J Am Soc Hortic Sci 116:142–148

    CAS  Google Scholar 

  • Quinn GP, Keough MJ (2002) Experimental design and data analysis for biologists. Cambridge University Press, Cambridge

    Google Scholar 

  • Rushforth K (1999) Trees of Britain and Europe. HarperCollins Publishers, London

    Google Scholar 

  • Trigiano RN, Beaty RM, Dietrich JT (1989) Somatic embryogenesis and plantlet regeneration in Cornus florida. Plant Cell Rep 8:270–273

    Article  Google Scholar 

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The author thanks Dr. D. Gömöry for statistical advice, Mrs. H. Parobková for preparation of culture media, and Mrs. A. Lengyelová for excellent care for plantlets transferred to ex vitro environment. This work was financed by the Slovak Grant Agency VEGA (1/3262/06 and 1/3514/06).

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Correspondence to Jaroslav Ďurkovič.

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Communicated by D. Treutter.

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Ďurkovič, J. Micropropagation of mature Cornus mas ‘Macrocarpa’. Trees 22, 597–602 (2008).

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