Abstract
The occurrence of functional chloroplasts in internal stem tissues and their distribution profiles in 20 woody species have been investigated. Chloroplasts were identified from the red chlorophyll auto-fluorescence using epi-fluorescence microscopy. Chloroplasts were detected in the cortex of all species examined, in the xylem rays of 19 and in the perimedullar and the pith cells of 16 out of the 20 investigated species. Chloroplast containing cell clusters in the pith were identified in some species. In addition, we report on the semi-quantitative distribution of chlorophylls in various internal stem tissues. Chlorophyll level was estimated by reflectance measurements at specific wave bands. Although decreasing chlorophyll gradients from cortex to pith were observed in half of the species, chlorophyll distribution in the remaining species was irregular with occasionally high levels in the pith. According to our data, chlorophyll occurrence in stem internal tissues is quite widespread, even in the light remote, deeply shaded central compartments like pith, provided that corresponding cells are viable. The species-specific tissue distribution of chlorophyll levels may be used to select suitable plants to investigate further this neglected area of photosynthesis research.
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References
Buns R, Acker G, Beck E (1993) The plastids of the Yew Tree (Taxus baccata L.): ultrastructure and immunocytochemical examination of chloroplastic enzymes. Botanica Acta 106:32–41
Fukshansky L (1978) Theory of light absorption in nonhomogeneous objects—sieve effect in one component suspensions. J Math Biol 6:177–196
Hawes C, Satiat-Jeunemaitre B (2001) Plant cell biology, 2nd edn. Oxford University Press, New York
Kauppi A (1991) Seasonal fluctuations in chlorophyll content in birch stems with special reference to bark thickness and light transmission, a comparison between sprouts and seedlings. Flora 185:107–125
Krause GH, Weiss E (1984) Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals. Photosynthesis Res 5:139–157
Langenfeld-Heyser R (1989) CO2 fixation in stem slices of Picea abies L. Karst: microautoradiographic studies. Trees-Struct Funct 3: 24–32
Larcher W, Lütz C, Nagele M, Bodner M (1988) Photosyntetic functioning and ultrastructure of chloroplasts in stem tissues of Fagus sylvatica. J Plant Physiol 132:731–737
Manetas Y (2004) Photosynthesizing in the rain: beneficial effects of twig wetting on corticular photosynthesis through changes in the periderm optical properties. Flora 199:334–341
Manetas Y, Pfanz H (2005) Spatial heterogeneity of light penetration through periderm and lenticels and concomitant patchy acclimation of corticular photosynthesis. Trees, Published on line: DOI 10.1007/s00468-044-0399-7
Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668.
Peñuelas J, Filella I (1998) Visible and near-infrared reflectance techniques for diagnosing plant physiological status. Trends Plant Sci 3:151–156
Pfanz H, Aschan G (2001) The existence of bark and stem photosynthesis in woody plants and its significance for the overall carbon gain. An eco-physiological and ecological approach. Prog Bot 62:477–510
Pfanz H, Aschan G, Langenfeld-Heyser R, Witmann C, Loose M (2002) Ecology and ecophysiology of tree stems: corticular and wood photosynthesis. Naturwissenschaften 89:147–162
Redondo-Gómez S, Wharmby C, Moreno FJ, De Cires A, Castillo JM, Luque T, Davy AJ, Figueroa ME (2005) Presence of internal photosynthetic cylinder surrounding the stele in stems of the tribe Salicorniae (Chenopodiaceae) from SW Iberian Peninsula. Photosynthetica 43:157–159
Scott DJ (1907) On the distribution of chlorophyll in the young shoots of woody plants. Ann Bot 21:437–439
Solhaug KA, Gauslaa Y, Haugen J (1995) Adverse effects of epiphytic crustose lichens upon stem photosynthesis and chlorophyll of Populus tremula L. Botanica Acta 108:233–239
Sun Q, Yoda K, Suzuki M, Suzuki H (2003) Vascular tissue in the stem and roots of woody plants can conduct light. J Exp Bot 54:1627–1635
Van Cleve B, Forreiter C, Sauter JJ, Apel K (1993) Pith cells of poplar contain phosynthetically active chloroplasts. Planta 189:70–73
Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144:307–313
Wiebe H (1975) Photosynthesis in wood. Physiol Plant 33:245–246
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Dima, E., Manetas, Y. & Psaras, G.K. Chlorophyll distribution pattern in inner stem tissues: evidence from epifluorescence microscopy and reflectance measurements in 20 woody species. Trees 20, 515–521 (2006). https://doi.org/10.1007/s00468-006-0067-1
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DOI: https://doi.org/10.1007/s00468-006-0067-1