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Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock

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Abstract

Brassinosteroids (BRs) comprise a specific class of low-abundance plant steroids now recognized as a new class of phytohormones. In this paper, we demonstrate that a fluoro derivative of 28-homocastasterone (5F-HCTS) stimulates branch elongation in in vitro-grown shoots of Malus prunifolia, the marubakaido apple rootstock. In addition to that, we show that this BR-stimulated branch elongation is paralleled by an increase in ethylene release. However, either the presence of 1-amino-cyclopropane-1-carboxylic acid (ACC), the immediate precursor of ethylene in higher plants, in the culture medium or an ethylene-enriched atmosphere resulted in inhibition of branch elongation, indicating that the stimulation of branch elongation observed for 5F-HCTS-treated shoots in this study was not, at least directly, related to the BR-induced enhancement in ethylene release rate. Besides its positive effect on the marubakaido shoot growth, i.e. branch elongation, the 5F-HCTS-driven enhancement of branch elongation found in this study is potentially useful to improve micropropagation techniques for other plant species as well, especially woody species, in which branch elongation is typically a constraint for efficient micropropagation.

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Acknowledgements

“Conselho Nacional de Pesquisa e Desenvolvimento”-Brazil is acknowledged for the fellowships granted to the senior author, S. Shaefer, S. A. Medeiro and C. T. A. C. Silva. Drs. F. Pedrosa, E. M. Souza and G. Klassen are acknowledged for their assistance with the gas chromatography

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Authors and Affiliations

  1. Department of Botany-SCB, Centro Politecnico-UFPR, C.P. 19031, Curitiba, PR, 81531-970, Brazil

    A. B. Pereira-Netto, C. T. A. Cruz-Silva & S. Schaefer

  2. Department of Organic Chemistry, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 1428, Buenos Aires, Argentina

    J. A. Ramírez & L. R. Galagovsky

Authors
  1. A. B. Pereira-Netto
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  2. C. T. A. Cruz-Silva
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  3. S. Schaefer
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  4. J. A. Ramírez
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  5. L. R. Galagovsky
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Correspondence to A. B. Pereira-Netto.

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Pereira-Netto, A.B., Cruz-Silva, C.T.A., Schaefer, S. et al. Brassinosteroid-stimulated branch elongation in the marubakaido apple rootstock. Trees 20, 286–291 (2006). https://doi.org/10.1007/s00468-005-0041-3

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  • DOI: https://doi.org/10.1007/s00468-005-0041-3

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