Skip to main content

Advertisement

SpringerLink
Log in

Application of genetic markers to the discrimination of European Black Poplar (Populus nigra) from American Black Poplar (P. deltoides) and Hybrid Poplars (P. x canadensis) in Switzerland

  • Original Article
  • Published:
Trees Aims and scope Submit manuscript

Abstract

European Black Poplar (Populus nigra) is considered a rare and endangered tree species because of severe reduction of its natural riverine habitat and potential hybridisation with the related non-indigenous taxa P. deltoides and P. x canadensis. As it is difficult to distinguish these taxa solely based on their morphology, we applied a PCR-based assay with an easy-to-use and robust molecular marker set (cpDNA trnL-trnF/RsaI RFLP, nDNA win3 and nDNA POPX/MspI RFLP) in order to identify pure P. nigra. Different plant tissues could be used for fast and standardised DNA extraction. The application of the three marker types was tested on a number of different Populus taxa, and they were also used for the verification of pure P. nigra in a sample of 304 putative P. nigra individuals from Switzerland. Cross-checking of the DNA data with those using a traditional allozyme approach resulted in complete agreement. The availability of molecular identification methods is an important prerequisite for the conservation of European Black Poplar, because pure, non-introgressed plant material can then be used in restoration projects of European floodplains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Arens P, Coops H, Jansen J, Vosman B (1998) Molecular genetic analysis of Black Poplar (Populus nigra L.) along Dutch rivers. Mol Ecol 7:11–18

    Article  CAS  Google Scholar 

  • Barsoum N (2001) Regeneration – requirements and promotion measures. In: Lefèvre F, Barsoum N, Heinze B, Kajba D, Rotach P, De Vries SMG, Turok J (eds) In situ conservation of Populus nigra. IPGRI, Rome, pp 16–24

    Google Scholar 

  • Benetka V, Mottl J, Vacková K, Pospíšková M, Dubsky M (1999) Estimation of the introgression level in Populus nigra L. populations by means of isozyme gene markers. Silvae Genet 48:218–223

    Google Scholar 

  • Bradshaw HD Jr, Villar M, Watson BD, Otto KG, Stewart S, Stettler RF (1994) Molecular genetics of growth and development in Populus. 3. A genetic linkage map of Hybrid Poplar composed of RFLP, STS and RAPD markers. Theor Appl Genet 89:167–178

    CAS  Google Scholar 

  • Cagelli L, Lefèvre F (1995) The conservation of Populus nigra and gene flow with cultivated poplars in Europe. Forest Genet 2:135–144

    Google Scholar 

  • Demesure B, Sodzi N, Petit RJ (1995) A set of universal primers for amplification of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants. Mol Ecol 4:129–131

    PubMed  CAS  Google Scholar 

  • Dietiker F, Jansen E (2003) Das Sachprogramm Auenschutzpark Aargau – eine Zwischenbilanz mit Beispielen realisierter Waldprojekte im Freiamt. Schweiz Z Forstwes 7:274–280

    Article  Google Scholar 

  • EUFORGEN (s.d.) Populus nigra L. IPGRI, Rome

  • Fossati T, Grassi F, Sala F, Castiglione S (2003) Molecular analysis of natural populations of Populus nigra L. intermingled with cultivated hybrids. Mol Ecol 12:2033–2043

    Article  PubMed  CAS  Google Scholar 

  • Heinze B (1997) A PCR marker for a Populus deltoides allele and its use in studying introgression with native European Populus nigra. Belg J Bot 129:123–130

    Google Scholar 

  • Heinze B (1998) Molekulargenetische Unterscheidung und Identifizierung von Schwarzpappeln und Hybridpappelklonen. FBVA Ber 105:1–44

    Google Scholar 

  • Heinze B (2001) PCR-RFLP analysis of introns of nuclear genes in Populus and Prunus. In: Müller-Starck G, Schubert R (eds) Genetic responses of forest systems to changing environmental conditions. Kluwer, Dordrecht, pp 117–127

    Google Scholar 

  • Heinze B, De Vries SMG (1998) Integration nationaler und internationaler Generhaltungsprogramme: das EUFORGEN – Netzwerk Schwarzpappel in Österreich und den Niederlanden. In: Geburek T, Heinze B (eds) Erhaltung genetischer Ressourcen im Wald – Normen, Programme, Massnahmen. Ecomed, Landsberg, pp 178–188

    Google Scholar 

  • Heinze B, Lefèvre F (2001) Genetic considerations for the restoration of riparian populations. In: Lefèvre F, Barsoum N, Heinze B, Kajba D, Rotach P, De Vries SMG, Turok J (eds) In situ conservation of Populus nigra. IPGRI, Rome, pp 25–35

    Google Scholar 

  • Heinze B, Lickl E (2002) Rare, but steady, introgression in Austrian Black Poplar as a long-term risk? In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 169–175

    Google Scholar 

  • Hofmann M (2002) Economic aspects of Black Poplar – hybrids, yield and utilisation of wood. In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 205–212

    Google Scholar 

  • Imbert E, Lefèvre F (2003) Dispersal and gene flow of Populus nigra (Salicaceae) along a dynamic river system. J Ecol 91:447–456

    Article  Google Scholar 

  • Janssen A (1997) Unterscheidung der beiden Schwarzpappelarten Populus nigra L. und P. deltoides Marsh. sowie ihrer Arthybride P. x euramericana (Dode) Guinier mit Hilfe von Isoenzymmustern. Holzzucht 51:17–23

    Google Scholar 

  • Kajba D, Romanić B (2002) Morphological leaf variability of the European Black Poplar (Populus nigra L.) in natural populations in the Drava river basin in Croatia. In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 221–227

    Google Scholar 

  • Lefèvre F, Kajba D, Heinze B, Rotach P, De Vries SMG, Turok J (2001) Black Poplar: a model for gene resource conservation in forest ecosystems. Forest Chron 77:139–244

    Google Scholar 

  • Lefèvre F, Bordács S, Cotrell J, Gebhardt K, Smulders MJM, Van den Broeck A, Vornam B, Van Dam BC (2002) Recommendations for riparian ecosystem management based on the general frame defined in EUFORGEN and results from EUROPOP. In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 157–161

    Google Scholar 

  • QIAGEN (2002) DNeasy 96 plant handbook. QIAGEN, Valencia

    Google Scholar 

  • Rajora OP (1989) Characterisation of 43 Populus nigra L. clones representing selections, cultivars and botanical varieties based on their multilocus allozyme genotypes. Euphytica 43:197–206

    Article  Google Scholar 

  • Rajora OP, Dancik BP (1992) Chloroplast DNA inheritance in Populus. Theor Appl Genet 84:280–285

    Google Scholar 

  • Rajora OP, Rahman MH (2001) Microsatellite DNA markers and their usefulness in poplars and conservation of microsatellite DNA loci in Salicaceae. In: Müller-Starck G, Schubert R (eds) Genetic response of forest systems to changing environmental conditions. Kluwer, Dordrecht, pp 105–115

    Google Scholar 

  • Rajora OP, Rahman MH (2003) Microsatellite DNA and RAPD fingerprinting identification and genetic relationships of Hybrid Poplar (Populus x canadensis) cultivars. Theor Appl Genet 106:470–477

    PubMed  CAS  Google Scholar 

  • Rohde S, Kienast F, Bürgi M (2004) Assessing the rstoration success of river widenings: a landscape approach. Environ Manage 34:574–589

    Article  PubMed  Google Scholar 

  • Rotach P (2001) General considerations and basic strategies. In: Lefèvre F, Barsoum N, Heinze B, Kajba D, Rotach P, De Vries SMG, Turok J (eds) In situ conservation of Populus nigra. IPGRI, Rome, pp 8–15

    Google Scholar 

  • Storme V, Van den Broeck A, Ivens B, Halfmaerten D, Van Slycken J, Castiglione S, Grassi F, Fossati T, Cottrell JE, Tabbener HE, Lefèvre F, Saintagne C, Fluch S, Krystufek V, Burg K., Bordásc S, Borovics A, Gebhardt K, Vornam B, Pohl A, Alba N, Agúdez D, Maestro C, Notivol E, Bovenschen J, Van Dam BC, Van der Schoot J, Vosman B, Boerjan W, Smulders MJM (2004) Ex-situ conservation of Black Poplar in Europe: genetic diversity in nine gene bank collections and their value for nature development. Theor Appl Genet 108:969–981

    Article  PubMed  CAS  Google Scholar 

  • Tabbener HE, Cotrell JE (2003) The use of PCR based DNA markers to study the paternity of poplar seedlings. Forest Ecol Manage 179:363–376

    Article  Google Scholar 

  • Van Dam BC (2002) EUROPOP: genetic diversity in river populations of European Black Poplar for evaluation of biodiversity, conservation strategies, nature development and genetic improvement. In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 15–32

    Google Scholar 

  • Van den Broeck AH, Storme V, Cox K, Quataert P, Ivens B, Hostyn V, Halfmaerten D, Boerjan W, Van Slycken J (2002) Mating system of Populus nigra L. in a mixed poplar stand composed of P. nigra, P. deltoides and P. x euramericana. In: Van Dam BC, Bordács S (eds) Genetic diversity in river populations of European Black Poplar. Csiszár Nyomda, Budapest, pp 61–72

    Google Scholar 

  • Van den Broeck A, Quataert P, Roldán-Ruiz I, Van Bockstaele E, Van Slycken J (2003) Pollen competition in Populus nigra females revealed by microsatellite markers. Forest Genet 10:219–227

    Google Scholar 

  • Van der Schoot J, Pospíšková M, Vosman B, Smulders MJM (2000) Development and characterisation of microsatellite markers in Black Poplar (Populus nigra L.). Theor Appl Genet 101:317–322

    Article  Google Scholar 

  • Van Slycken J (1995) Plant descriptors for Populus nigra. In: Turok J, Lefèvre F, Cagelli L, De Vries S (eds) Populus nigra network second meeting. IPGRI, Rome, pp 13–24

    Google Scholar 

  • Vornam B, Herzog S, Preisigmuller R, Hattemer HH (1994) Restriction-fragment-length-polymorphisms of a chloroplast photosystem-II gene from poplar and their use for species identification. Genome 37:747–750

    CAS  Google Scholar 

  • Wendel JF, Weeden NF (1989) Visualisation and interpretation of plant isozymes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides, Portland, pp 5–45

    Google Scholar 

  • Winfield MO, Arnold GM, Cooper F, Le Ray M, White J, Karp A, Edwards KJ (1998) A study of genetic diversity in Populus nigra subsp. betulifolia in the Upper Severn area of the UK using AFLP markers. Mol Ecol 7:3–10

    Article  CAS  Google Scholar 

Download references

Acknowledgements

We thank Annie Diarra, Fabienne Bourquin and Amira Sepic for help in the laboratory, Peter Lawrenz for the sampling of the blind test poplars and many foresters and forest authorities, especially Markus Bolliger, for their support. We would also like to thank Patrick Bonfils, Andreas Rudow, Peter Rotach, Peter Schwab und Marcus Ulber for help throughout the work. This research was financed by the BUWAL/Swiss Federal Forest Direction. Finally, we thank Ivana Stehlik, Berthold Heinze and an anonymous referee for critical comments on the manuscript

Author information

Authors and Affiliations

  1. WSL Swiss Federal Research Institute, Zürcherstrasse 111, CH-8903, Birmensdorf, Switzerland

    R. Holderegger, S. Angelone, S. Brodbeck, D. Csencsics, F. Gugerli & S. E. Hoebee

  2. Institute of Forest Genetics and Forest Tree Breeding, Georg-August University Göttingen, Büsgenweg 2, D-37077, Göttingen, Germany

    R. Finkeldey

Authors
  1. R. Holderegger
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Angelone
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Brodbeck
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. Csencsics
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. Gugerli
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. E. Hoebee
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. Finkeldey
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. Holderegger.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Holderegger, R., Angelone, S., Brodbeck, S. et al. Application of genetic markers to the discrimination of European Black Poplar (Populus nigra) from American Black Poplar (P. deltoides) and Hybrid Poplars (P. x canadensis) in Switzerland. Trees 19, 743–748 (2005). https://doi.org/10.1007/s00468-005-0006-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00468-005-0006-6

Keywords

Search

Navigation