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In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae)

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Abstract

A protocol for the in vitro regeneration of rooted plants from nodal single bud segments of 10-year-old Schinopsis balansae trees was developed. Nodal segments were harvested from actively growing shoots of plants grown from seeds and maintained in pots under greenhouse conditions, and from epicormic shoots obtained by forced flushing of branches. Culture of nodal segments on nutrient medium containing the mineral salts and vitamins of Murashige and Skoog medium at 1/4 strength (1/4 MS), supplemented with 100 mg l−1 ascorbic acid, 3% sucrose, and 5–15 μM 6-benzyladenine resulted in regeneration of multiple shoots. Rooting of regenerated shoots was observed in 1/4 MS medium with vermiculite as the substrate and supplemented with 7.5 μM indolbutyric acid.

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Acknowledgements

This research was supported by Secretaría de Ciencia y Técnica (UNNE). The authors wish to thank Unitan for their cooperation with this work. The authors also to thank Aldo A. Goytía for the photographs.

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  1. Facultad de Ciencias Agrarias (UNNE), Instituto de Botánica del Nordeste (IBONE), C.C. 209, 3400, Corrientes, Argentina

    Pedro Sansberro, Hebe Rey, Luis Mroginski & Claudia Luna

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  1. Pedro Sansberro
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  2. Hebe Rey
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  3. Luis Mroginski
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  4. Claudia Luna
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Correspondence to Pedro Sansberro.

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Sansberro, P., Rey, H., Mroginski, L. et al. In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae). Trees 17, 542–546 (2003). https://doi.org/10.1007/s00468-003-0271-1

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  • DOI: https://doi.org/10.1007/s00468-003-0271-1

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