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CDKA orthologue isolation and its expression during cambial activity in hybrid walnut (Juglans nigra×Juglans regia)

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Abstract

Improved wood production is a major aim for walnut forests and especially for hybrid walnut (Juglans nigra×Juglans regia). This paper presents the first report describing the structure and function of a CDKA gene potentially involved in radial growth and wood production in walnut. We report the isolation of the full sequence of a CDKA gene by the use of degenerate primers and RACE approaches. We also describe the expression of the CDKA gene during radial growth. The analysis of intron sizes and positions as well as Southern-blot analysis suggest the presence of multiple copies (more than 2) of the CDKA gene in the hybrid walnut genome. Two different promoter sequences were cloned by the use of two different second-intron-specific primers.

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Acknowledgements

We are grateful to Eric Germain and Joëlle Chat for supplying genetic material. Christian Breton and Catherine Bergounioux are gratefully acknowledged for helpful discussions. Referees are deeply thanked for their contribution to the improvement of the manuscript. This work was funded by a European contract, FAIR CT96–1887, and financial assistance by Le Conseil Régional de la Région-Centre.

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  1. Unité Amélioration Génétique et Physiologie Forestières, Institut National de la Recherche Agronomique, Avenue de la Pomme de Pin, BP 20619 Ardon, 45166 Olivet cedex, France

    Nadia Goué, Grégory Montiel, Isabelle Levert, Muriel Gaudet, Christian Jay-Allemand & Philippe Label

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  1. Nadia Goué
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  2. Grégory Montiel
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Correspondence to Philippe Label.

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Goué, N., Montiel, G., Levert, I. et al. CDKA orthologue isolation and its expression during cambial activity in hybrid walnut (Juglans nigra×Juglans regia). Trees 17, 316–324 (2003). https://doi.org/10.1007/s00468-002-0241-z

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