Abstract
Background
Serum creatinine concentration is a primary component of Bedside Schwartz equation for estimated glomerular filtration rate (eGFR) in children. To standardize creatinine measurement, most manufacturers have adopted calibration procedures traceable to isotope dilution mass spectrometry (IDMS) using National Institute of Standards and Technology reference material. However, reference material representing much lower creatinine concentrations seen in children is not available and it is unclear how well commercial assays perform at pediatric levels.
Methods
One thousand nine hundred seventy-one specimens from consecutive children <19 years, with creatinine ≤0.8 mg/dL by Abbott Jaffe method were included. Creatinine measurements were compared between Abbott-Jaffe and Abbott-enzymatic methods. Furthermore, we evaluated performance of six commercial creatinine assays at concentrations seen in pediatric patients utilizing IDMS traceable serum samples.
Results
Median difference (enzymatic-Jaffe) for prepubertal females was –0.18 mg/dL (2.5%tile, 97.5%tile: –0.30, –0.06), –0.12 mg/dL (–0.25, –0.00) for pubertal females, –0.17 mg/dL (–0.30, –0.04) for prepubertal males, –0.11 mg/dL (–0.24, 0.01) for pubertal males. Bias appeared proportional for each subgroup and decreased as creatinine concentrations increased. Using IDMS traceable samples, the greatest inter-assay variability was seen with the lowest creatinine levels (target 0.273 mg/dL), where 67% (4/6) of methods failed to reach minimal bias specification of 8% (range –7.5 to 86%). For samples with higher creatinine targets (0.440–0.634 mg/dL), two methods failed to meet minimal bias specification, whereas four showed bias <8%.
Conclusion
Many commonly used creatinine assays remain inaccurate for pediatric populations after over a decade of nationwide efforts to standardize measurements. When creatinine-based eGFR is used for chronic kidney disease (CKD) staging in children, large inter-assay variability can lead to disease misclassification, inappropriate diagnostic and therapeutic interventions.
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Funding
The authors would like to thank Kelly Walewski and Robin Carey-Ballough from Beaumont Health at Royal Oak, and Angie Baldwin from the University of Chicago for their technical support.
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The investigation was approved by the Institutional Review Board at Beaumont Health (#2021-178).
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The authors declare no competing interests.
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Lao, K., Sykes, E., van Wijk, X.M.R. et al. Large inter-assay difference of serum creatinine in pediatric population: a threat to accurate staging of chronic kidney disease. Pediatr Nephrol 37, 677–681 (2022). https://doi.org/10.1007/s00467-021-05335-x
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DOI: https://doi.org/10.1007/s00467-021-05335-x