Abstract
The selectivity of the glomerular filter is established by physical, chemical, and signaling interplay among its three core constituents: glomerular endothelial cells, the glomerular basement membrane, and podocytes. Functional impairment or injury of any of these three components can lead to proteinuria. Podocytes are injured in many forms of human and experimental glomerular disease, including minimal change disease, focal segmental glomerulosclerosis, and diabetes mellitus. One of the earliest signs of podocyte injury is loss of their distinct structure, which is driven by dysregulated dynamics of the actin cytoskeleton. The status of the actin cytoskeleton in podocytes depends on a set of actin binding proteins, nucleators and inhibitors of actin polymerization, and regulatory GTPases. Mutations that alter protein function in each category have been implicated in glomerular diseases in humans and animal models. In addition, a growing body of studies suggest that pharmacological modifications of the actin cytoskeleton have the potential to become novel therapeutics for podocyte-dependent chronic kidney diseases. This review presents an overview of the essential proteins that establish actin cytoskeleton in podocytes and studies demonstrating the feasibility of drugging actin cytoskeleton in kidney diseases.
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Pavenstädt H, Kriz W, Kretzler M (2003) Cell biology of the glomerular podocyte. Physiol Rev 83:253–307
Kerjaschki D (2001) Caught flat-footed: podocyte damage and the molecular bases of focal glomerulosclerosis. J Clin Invest 108:1583–1587
Reiser J, von Gersdorff G, Simons M, Schwarz K, Faul C, Giardino L, Heider T, Loos M, Mundel P (2002) Novel concepts in understanding and management of glomerular proteinuria. Nephrol Dial Transplant 17:951–955
Saleem MA, O'Hare MJ, Reiser J, Coward RJ, Inward CD, Farren T, Xing CY, Ni L, Mathieson PW, Mundel P (2002) A conditionally immortalized human podocyte cell line demonstrating nephrin and podocin expression. J Am Soc Nephrol 13:630–638
Kistler AD, Singh G, Pippin J, Altintas MM, Yu H, Fernandez IC, Gu C, Wilson C, Srivastava SK, Dietrich A, Walz K, Kerjaschki D, Ruiz P, Dryer S, Sever S, Dinda AK, Faul C, Reiser J (2013) TRPC6 protects podocytes during complement-mediated glomerular disease. J Biol Chem 288:36598–36609
Reiser J, Sever S (2013) Podocyte biology and pathogenesis of kidney disease. Ann Rev Med 64:357–366
Reiser J, Sever S, Faul C (2014) Signal transduction in podocytes--spotlight on receptor tyrosine kinases. Nat Rev Nephrol 10:104–115
Brinkkoetter PT, Ising C, Benzing T (2013) The role of the podocyte in albumin filtration. Nat Rev Nephrol 9:328–336
Butt L, Unnersjö-Jess D, Höhne M, Edwards A, Binz-Lotter J, Reilly D, Hahnfeldt R, Ziegler V, Fremter K, Rinschen MM, Helmstädter M, Ebert LK, Castrop H, Hackl MJ, Walz G, Brinkkoetter PT, Liebau MC, Tory K, Hoyer PF, Beck BB, Brismar H, Blom H, Schermer B, Benzing T (2020) A molecular mechanism explaining albuminuria in kidney disease. Nat Metab 2:461–474
Benzing T (2020) Molecular design of the kidney filtration barrier. Trans Am Clin Climatol Assoc 131:125–139
Santin S, García-Maset R, Ruíz P, Giménez I, Zamora I, Peña A, Madrid A, Camacho JA, Fraga G, Sánchez-Moreno A, Cobo MA, Bernis C, Ortiz A, de Pablos AL, Pintos G, Justa ML, Hidalgo-Barquero E, Fernández-Llama P, Ballarín J, Ars E, Torra R, FSGS Spanish Study Group (2009) Nephrin mutations cause childhood- and adult-onset focal segmental glomerulosclerosis. Kidney Int 76:1268–1276
Shih NY, Li J, Karpitskii V, Nguyen A, Dustin ML, Kanagawa O, Miner JH, Shaw AS (1999) Congenital nephrotic syndrome in mice lacking CD2-associated protein. Science 286:312–315
Kaplan JM, Kim SH, North KN, Rennke H, Correia LA, Tong HQ, Mathis BJ, Rodríguez-Pérez JC, Allen PG, Beggs AH, Pollak MR (2000) Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis. Nat Genet 24:251–256
Brown EJ, Schlondorff JS, Becker DJ, Tsukaguchi H, Tonna SJ, Uscinski AL, Higgs HN, Henderson JM, Pollak MR (2010) Mutations in the formin gene INF2 cause focal segmental glomerulosclerosis. Nat Genet 42:72–76
Boyer O, Benoit G, Gribouval O, Nevo F, Tete MJ, Dantal J, Gilbert-Dussardier B, Touchard G, Karras A, Presne C, Grunfeld JP, Legendre C, Joly D, Rieu P, Mohsin N, Hannedouche T, Moal V, Gubler MC, Broutin I, Mollet G, Antignac C (2011) Mutations in INF2 are a major cause of autosomal dominant focal segmental glomerulosclerosis. J Am Soc Nephrol 22:239–245
Faul C, Asanuma K, Yanagida-Asanuma E, Kim K, Mundel P (2007) Actin up: regulation of podocyte structure and function by components of the actin cytoskeleton. Trends Cell Biol 17:428–437
Sever S, Schiffer M (2018) Actin dynamics at focal adhesions: a common endpoint and putative therapeutic target for proteinuric kidney diseases. Kidney Int 93:1298–1307
Martin CE, Jones N (2018) Nephrin signaling in the podocyte: an updated view of signal regulation at the slit diaphragm and beyond. Front Endocrinol (Lausanne) 9:302
Perico L, Conti S, Benigni A, Remuzzi G (2016) Podocyte-actin dynamics in health and disease. Nat Rev Nephrol 12:692–710
Case LB, Waterman CM (2015) Integration of actin dynamics and cell adhesion by a three-dimensional, mechanosensitive molecular clutch. Nat Cell Biol 17:955–963
Spiering D, Hodgson L (2011) Dynamics of the rho-family small GTPases in actin regulation and motility. Cell Adhes Migr 5:170–180
Basile DP, Dwinell MR, Wang SJ, Shames BD, Donohoe DL, Chen S, Sreedharan R, Van Why SK (2013) Chromosome substitution modulates resistance to ischemia reperfusion injury in Brown Norway rats. Kidney Int 83:242–250
Blattner SM, Hodgin JB, Nishio M, Wylie SA, Saha J, Soofi AA, Vining C, Randolph A, Herbach N, Wanke R, Atkins KB, Gyung Kang H, Henger A, Brakebusch C, Holzman LB, Kretzler M (2013) Divergent functions of the Rho GTPases Rac1 and Cdc42 in podocyte injury. Kidney Int 84:920–930
Akilesh S, Suleiman H, Yu H, Stander MC, Lavin P, Gbadegesin R, Antignac C, Pollak M, Kopp JB, Winn MP, Shaw AS (2011) Arhgap24 inactivates Rac1 in mouse podocytes, and a mutant form is associated with familial focal segmental glomerulosclerosis. J Clin Invest 121:4127–4137
Gee HY, Saisawat P, Ashraf S, Hurd TW, Vega-Warner V, Fang H, Beck BB, Gribouval O, Zhou W, Diaz KA, Natarajan S, Wiggins RC, Lovric S, Chernin G, Schoeb DS, Ovunc B, Frishberg Y, Soliman NA, Fathy HM, Goebel H, Hoefele J, Weber LT, Innis JW, Faul C, Han Z, Washburn J, Antignac C, Levy S, Otto EA, Hildebrandt F (2013) ARHGDIA mutations cause nephrotic syndrome via defective RHO GTPase signaling. J Clin Invest 123:3243–3253
Sugano Y, Lindenmeyer MT, Auberger I, Ziegler U, Segerer S, Cohen CD, Neuhauss SCF, Loffing J (2015) The Rho-GTPase binding protein IQGAP2 is required for the glomerular filtration barrier. Kidney Int 88:1047–1056
Sever S, Altintas MM, Nankoe SR, Moller CC, Ko D, Wei C, Henderson J, del Re EC, Hsing L, Erickson A, Cohen CD, Kretzler M, Kerjaschki D, Rudensky A, Nikolic B, Reiser J (2007) Proteolytic processing of dynamin by cytoplasmic cathepsin L is a mechanism for proteinuric kidney disease. J Clin Invest 117:2095–2104
Soda K, Balkin DM, Ferguson SM, Paradise S, Milosevic I, Giovedi S, Volpicelli-Daley L, Tian X, Wu Y, Ma H, Son SH, Zheng R, Moeckel G, Cremona O, Holzman LB, De Camilli P, Ishibe S (2012) Role of dynamin, synaptojanin, and endophilin in podocyte foot processes. J Clin Invest 122:4401–4411
Sever S, Chang J, Gu C (2013) Dynamin rings: not just for fission. Traffic 14:1194–1199
Reubold TF, Faelber K, Plattner N, Posor Y, Ketel K, Curth U, Schlegel J, Anand R, Manstein DJ, Noé F, Haucke V, Daumke O, Eschenburg S (2015) Crystal structure of the dynamin tetramer. Nature 525:404–408
Sever S, Muhlberg AB, Schmid SL (1999) Impairment of dynamin's GAP domain stimulates receptor-mediated endocytosis. Nature 398:481–486
Binns DD, Helms MK, Barylko B, Davis CT, Jameson DM, Albanesi JP, Eccleston JF (2000) The mechanism of GTP hydrolysis by dynamin II: a transient kinetic study. Biochemistry 39:7188–7196
Gu C, Yaddanapudi S, Weins A, Osborn T, Reiser J, Pollak M, Hartwig J, Sever S (2010) Direct dynamin-actin interactions regulate the actin cytoskeleton. EMBO J 29:3593–3606
Gu C, Chang J, Shchedrina VA, Pham VA, Hartwig JH, Suphamungmee W, Lehman W, Hyman BT, Bacskai BJ, Sever S (2014) Regulation of dynamin oligomerization in cells: the role of dynamin-actin interactions and its GTPase activity. Traffic 15:819–838
Gu C, Lee HW, Garborcauskas G, Reiser J, Gupta V, Sever S (2017) Dynamin autonomously regulates podocyte focal adhesion maturation. J Am Soc Nephrol 28:446–451
Schiffer M, Teng B, Gu C, Shchedrina VA, Kasaikina M, Pham VA, Hanke N, Rong S, Gueler F, Schroder P, Tossidou I, Park JK, Staggs L, Haller H, Erschow S, Hilfiker-Kleiner D, Wei C, Chen C, Tardi N, Hakroush S, Selig MK, Vasilyev A, Merscher S, Reiser J, Sever S (2015) Pharmacological targeting of actin-dependent dynamin oligomerization ameliorates chronic kidney disease in diverse animal models. Nat Med 21:601–609
Rollason R, Wherlock M, Heath JA, Heesom KJ, Saleem MA, Welsh GI (2016) Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ alpha-1) and profilin 2. Biosci Rep 36:e00302
Schell C, Sabass B, Helmstaedter M, Geist F, Abed A, Yasuda-Yamahara M, Sigle A, Maier JI, Grahammer F, Siegerist F, Artelt N, Endlich N, Kerjaschki D, Arnold HH, Dengjel J, Rogg M, Huber TB (2018) ARP3 controls the podocyte architecture at the kidney filtration barrier. Dev Cell 47:741–757 e8
Sun H, Al-Romaih KI, MacRae CA, Pollak MR (2014) Human kidney disease-causing INF2 mutations perturb Rho/Dia signaling in the glomerulus. EBioMedicine 1:107–115
Hohenstein B, Kasperek L, Kobelt DJ, Daniel C, Gambaryan S, Renné T, Walter U, Amann KU, Hugo CP (2005) Vasodilator-stimulated phosphoprotein-deficient mice demonstrate increased platelet activation but improved renal endothelial preservation and regeneration in passive nephrotoxic nephritis. J Am Soc Nephrol 16:986–996
Arrondel C, Vodovar N, Knebelmann B, Grünfeld JP, Gubler MC, Antignac C, Heidet L (2002) Expression of the nonmuscle myosin heavy chain IIA in the human kidney and screening for MYH9 mutations in Epstein and Fechtner syndromes. J Am Soc Nephrol 13:65–74
Johnstone DB, Zhang J, George B, Leon C, Gachet C, Wong H, Parekh R, Holzman LB (2011) Podocyte-specific deletion of Myh9 encoding nonmuscle myosin heavy chain 2A predisposes mice to glomerulopathy. Mol Cell Biol 31:2162–2170
Mele C, Iatropoulos P, Donadelli R, Calabria A, Maranta R, Cassis P, Buelli S, Tomasoni S, Piras R, Krendel M, Bettoni S, Morigi M, Delledonne M, Pecoraro C, Abbate I, Capobianchi MR, Hildebrandt F, Otto E, Schaefer F, Macciardi F, Ozaltin F, Emre S, Ibsirlioglu T, Benigni A, Remuzzi G, Noris M, PodoNet Consortium (2011) MYO1E mutations and childhood familial focal segmental glomerulosclerosis. N Engl J Med 365:295–306
Campellone KG, Welch MD (2010) A nucleator arms race: cellular control of actin assembly. Nat Rev Mol Cell Biol 11:237–251
Goley ED, Welch MD (2006) The ARP2/3 complex: an actin nucleator comes of age. Nat Rev Mol Cell Biol 7:713–726
Hegsted A, Yingling CV, Pruyne D (2017) Inverted formins: a subfamily of atypical formins. Cytoskeleton (Hoboken) 74:405–419
Sun H, Schlondorff J, Higgs HN, Pollak MR (2013) Inverted formin 2 regulates actin dynamics by antagonizing rho/diaphanous-related formin signaling. J Am Soc Nephrol 24:917–929
Mizuno H, Watanabe N (2012) mDia1 and formins: screw cap of the actin filament. Biophysics (Nagoya-shi) 8:95–102
Subramanian B, Sun H, Yan P, Charoonratana VT, Higgs HN, Wang F, Lai KV, Valenzuela DM, Brown EJ, Schlöndorff JS, Pollak MR (2016) Mice with mutant Inf2 show impaired podocyte and slit diaphragm integrity in response to protamine-induced kidney injury. Kidney Int 90:363–372
Lee HW, Khan SQ, Faridi MH, Wei C, Tardi NJ, Altintas MM, Elshabrawy HA, Mangos S, Quick KL, Sever S, Reiser J, Gupta V (2015) A Podocyte-based automated screening assay identifies protective small molecules. J Am Soc Nephrol 26:2741–2752
Li M, Armelloni S, Zennaro C, Wei C, Corbelli A, Ikehata M, Berra S, Giardino L, Mattinzoli D, Watanabe S, Agostoni C, Edefonti A, Reiser J, Messa P, Rastaldi MP (2014) BDNF repairs podocyte damage by microRNA-mediated increase of actin polymerization. J Pathol 235:731–744
Tian X, Ishibe S (2016) Targeting the podocyte cytoskeleton: from pathogenesis to therapy in proteinuric kidney disease. Nephrol Dial Transplant 31:1577–1583
Shi J, Wei L (2013) Rho kinases in cardiovascular physiology and pathophysiology: the effect of fasudil. J Cardiovasc Pharmacol 62:341–354
Komers R (2013) Rho kinase inhibition in diabetic kidney disease. Br J Clin Pharmacol 76:551–559
Babelova A, Jansen F, Sander K, Lohn M, Schafer L, Fork C, Ruetten H, Plettenburg O, Stark H, Daniel C, Amann K, Pavenstädt H, Jung O, Brandes RP (2013) Activation of Rac-1 and RhoA contributes to podocyte injury in chronic kidney disease. PLoS One 8:e80328
Nagatoya K, Moriyama T, Kawada N, Takeji M, Oseto S, Murozono T, Ando A, Imai E, Hori M (2002) Y-27632 prevents tubulointerstitial fibrosis in mouse kidneys with unilateral ureteral obstruction. Kidney Int 61:1684–1695
Kentrup D, Reuter S, Schnöckel U, Grabner A, Edemir B, Pavenstädt H, Schober O, Schäfers M, Schlatter E, Büssemaker E (2011) Hydroxyfasudil-mediated inhibition of ROCK1 and ROCK2 improves kidney function in rat renal acute ischemia-reperfusion injury. PLoS One 6:e26419
Huang J, Dey R, Wang Y, Jakoncic J, Kurinov I, Huang XY (2018) Structural insights into the induced-fit inhibition of fascin by a small-molecule inhibitor. J Mol Biol 430:1324–1335
Tandon R, Levental I, Huang C, Byfield FJ, Ziembicki J, Schelling JR, Bruggeman LA, Sedor JR, Janmey PA, Miller RT (2007) HIV infection changes glomerular podocyte cytoskeletal composition and results in distinct cellular mechanical properties. Am J Physiol Renal Physiol 292:F701–F710
Kliewe F, Scharf C, Rogge H, Darm K, Lindenmeyer MT, Amann K, Cohen CD, Endlich K, Endlich N (2017) Studying the role of fascin-1 in mechanically stressed podocytes. Sci Rep 7:9916
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S. S. is the co-founder and shareholder of Trisaq, a biotechnology company that develops novel kidney-protective therapies.
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Sever, S. Role of actin cytoskeleton in podocytes. Pediatr Nephrol 36, 2607–2614 (2021). https://doi.org/10.1007/s00467-020-04812-z
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DOI: https://doi.org/10.1007/s00467-020-04812-z