Pediatric Nephrology

, Volume 32, Issue 6, pp 1029–1033

Marked increase in urinary excretion of apolipoproteins in children with nephrolithiasis associated with hypercalciuria

  • Larisa Kovacevic
  • Hong Lu
  • Joseph A. Caruso
  • Tuhina Govil-Dalela
  • Ronald Thomas
  • Yegappan Lakshmanan
Original Article

DOI: 10.1007/s00467-016-3576-1

Cite this article as:
Kovacevic, L., Lu, H., Caruso, J.A. et al. Pediatr Nephrol (2017) 32: 1029. doi:10.1007/s00467-016-3576-1

Abstract

Background

Using a proteomic approach, we aimed to identify and compare the urinary excretion of proteins involved in lipid transport and metabolism in children with kidney stones and hypercalciuria (CAL), hypocitraturia (CIT), and normal metabolic work-up (NM), and in healthy controls (HCs). Additionally, we aimed to confirm these results using ELISA, and to examine the relationship between the urinary excretion of selected proteins with demographic, dietary, blood, and urinary parameters.

Methods

Prospective, controlled, pilot study of pooled urine from CAL, CIT, and NM versus age- and gender-matched HCs, using liquid chromatography-mass spectrometry. Relative protein abundance was estimated using spectral counting. Results were confirmed by ELISA performed on individual samples.

Results

Of the 1,813 proteins identified, 230 met the above criteria. Of those, 5 proteins (apolipoprotein A-II [APOA2]; apolipoprotein A-IV [APOA4]; apolipoprotein C-III [APOA3]; fatty acid-binding protein, liver [FABPL]; fatty acid-binding protein, adipocyte [FABP4]) involved in lipid metabolism and transport were found in the CAL group, with significant differences compared with HCs. ELISA analysis indicated statistically significant differences in the urinary excretion of APOC3, APOA4, and FABPL in the CAL group compared with HCs. Twenty-four-hour urinary calcium excretion correlated significantly with concentrations of ApoC3 (r = 0.77, p < 0.001), and FABPL (r = 0.80, p = 0.005).

Conclusions

We provide proteomic data showing increased urinary excretion of lipid metabolism/transport-related proteins in children with kidney stones and hypercalciuria. These findings suggest that abnormalities in lipid metabolism might play a role in kidney stone formation.

Keywords

Nephrolithiasis Urinary apolipoproteins Hypercalciuria Proteomics Children 

Funding information

Funder NameGrant NumberFunding Note
This research is being funded by the Children Hospital of Michigan Foundation R2-2014-31.
  • R2-2014-31
NIH Center Grant P30 ES 020957
  • P30 ES 020957

Copyright information

© IPNA 2017

Authors and Affiliations

  • Larisa Kovacevic
    • 1
  • Hong Lu
    • 1
  • Joseph A. Caruso
    • 2
  • Tuhina Govil-Dalela
    • 1
  • Ronald Thomas
    • 3
  • Yegappan Lakshmanan
    • 1
  1. 1.Department of Pediatric UrologyChildren’s Hospital of MichiganDetroitUSA
  2. 2.Institute of Environmental Health SciencesWayne State UniversityDetroitUSA
  3. 3.Department of StatisticsChildren’s Hospital of MichiganDetroitUSA

Personalised recommendations