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Plasma and urinary levels of cytokines in patients with idiopathic hypercalciuria

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Abstract

Background

Recent studies suggest that cytokines modulate bone turnover. Idiopathic hypercalciuria (IH) seems to be associated with bone mineral loss. Therefore, the aim of this study was to assess cytokines involved in bone turnover in patients with IH.

Methods

Plasma and spot-urine levels of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor alpha (TNF-α), transforming growth factor β1 (TGF-β1), and monocyte chemoattractant protein (MCP-1) were measured in 70 children and adolescents with IH and in 37 healthy controls. Patients with IH were subdivided according to their calciuria at the time of sample collection: ≥4 mg/kg/day (persistent IH, n=27) and below 4 mg/kg/day (controlled IH, n=43). Cytokines were determined by enzyme-linked immunoassay.

Results

Plasma and urinary concentrations of IL-1β, IL-6, IL-8, and TNF-α were undetectable in all groups. No differences were found between controlled and persistent hypercalciuria for plasma and urinary levels of MCP-1 and TGF-β1. On the other hand, MCP-1 levels were significantly higher in both subgroups of IH in comparison to healthy controls. Furthermore, urinary MCP-1 levels of IH patients correlated positively with bone mineral content (p=0.013).

Conclusion

Although cytokine measurements did not allow the differentiation between persistent and controlled IH, our findings suggest that MCP-1 might play a role in patients with IH.

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Acknowledgements

This study was partially supported by CNPq (Brazilian National Research Council) and FAPEMIG (Foundation of Research Support of Minas Gerais). Dr. A.C. Simões e Silva, Dr. E.A. Oliveira, Dr E.M. Lima, and Dr. M.M. Teixeira had a scientific productivity grant from the CNPq. Dr. A.C. Simões e Silva and Dr. E.A. Oliveira also received the Grant INCT-MM (FAPEMIG: CBB-APQ-00075-09 / CNPq 573646/2008-2).

Conflicts of interest

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Authors and Affiliations

  1. Pediatric Nephrology Unit, Department of Pediatrics, Faculty of Medicine, Belo Horizonte, MG, Brazil

    Augusto C. S. Santos Jr, Eleonora M. Lima, Maria Goretti M. G. Penido, Eduardo A. Oliveira & Ana Cristina Simões e Silva

  2. Laboratory of Immunopharmacology, Department of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, MG, Brazil

    Katia D. Silveira & Mauro M. Teixeira

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  1. Augusto C. S. Santos Jr
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  2. Eleonora M. Lima
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  6. Eduardo A. Oliveira
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  7. Ana Cristina Simões e Silva
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Correspondence to Ana Cristina Simões e Silva.

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Santos, A.C.S., Lima, E.M., Penido, M.G.M.G. et al. Plasma and urinary levels of cytokines in patients with idiopathic hypercalciuria. Pediatr Nephrol 27, 941–948 (2012). https://doi.org/10.1007/s00467-011-2094-4

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  • DOI: https://doi.org/10.1007/s00467-011-2094-4

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