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A comparative proteomic study of nephrogenesis in intrauterine growth restriction

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Abstract

Nephrogenesis requires a fine balance of many factors that can be disturbed by intrauterine growth restriction (IUGR), leading to a low nephron endowment. The aim of this study was to test the hypothesis that IUGR affects expression of key proteins that regulate nephrogenesis, by a comparative proteomic approach. IUGR was induced in Sprague–Dawley (SD) rats by isocaloric protein restriction in pregnant dams. A series of methods, including two-dimensional gel electrophoresis (2-DE), silver staining, mass spectrometry and database searching was used. After silver staining, 2-DE image analysis detected an average 730 ± 58 spots in the IUGR group and 711 ± 73 spots in the control group. The average matched rate was 86% and 81%, respectively. The differential proteomic expression analysis found that 11 protein spots were expressed only in the IUGR group and one in the control group. Seven protein spots were up-regulated more than fivefold and two were down-regulated more than fivefold in the IUGR group compared with those in control group. These 21 protein spots were preliminarily identified and were structural molecules, including vimentin, perlecan, gamma-actin and cytokeratin 10, transcription regulators, transporter proteins, enzymes, and so on. These proteins were involved primarily in energy metabolism, oxidation and reduction, signal transduction, cell proliferation and apoptosis. Data from this study may provide, at least partly, evidence that abnormality of metabolism, imbalance of redox and apoptosis, and disorder of cellular signal and cell proliferation may be the major mechanisms responsible for abnormal nephrogenesis in IUGR.

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Acknowledgements

This research was funded by the National Natural Science Foundation of China (NSFC, 30672242). We are grateful to Xiu-Rong Zhang and Zhong-Hua Zhao for their technical assistance.

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Authors and Affiliations

  1. Department of Nephrology and Rheumatology, Children’s Hospital of Fudan University, 399 Wan Yuan Road, Minhang District, Shanghai, 201102, People’s Republic of China

    Qian Shen, Hong Xu, Jing Chen, Hai-Mei Liu & Wei Guo

  2. Institutes for Biomedical Sciences, Fudan University, Shanghai, People’s Republic of China

    Li-Ming Wei

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  1. Qian Shen
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  2. Hong Xu
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  3. Li-Ming Wei
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Correspondence to Hong Xu.

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Shen, Q., Xu, H., Wei, LM. et al. A comparative proteomic study of nephrogenesis in intrauterine growth restriction. Pediatr Nephrol 25, 1063–1072 (2010). https://doi.org/10.1007/s00467-009-1437-x

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  • DOI: https://doi.org/10.1007/s00467-009-1437-x

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