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Vitamin E administration at the onset of fever prevents renal scarring in acute pyelonephritis

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Abstract

We evaluated the protective effects of antioxidant at the onset of fever on renal damage in a rat model of acute pyelonephritis. Twenty rats were allocated to four groups. In groups 1 to 3, the animals were given direct inoculation of Escherichia coli into the right kidney, and group four served as control. All rats in groups 1 to 3 were given once-daily intraperitoneal injections of ceftriaxon for five consecutive days, beginning on the third day after inoculation. The animals’ body temperatures were monitored; as soon as body temperature reaches 38°C, the rats in group 2 were given allopurinol co-treatment, whereas, in group 3, vitamin E co-treatment was started at fever onset. Both kidneys were excised 6 weeks later, for the evaluation of histopathologic changes, apoptotic damage, and concentrations of transforming growth factor-beta (TGF-β). Only minimal changes were found in control samples. Pathologic scores of inflammation and fibrosis in group 1 were higher than in the vitamin E and allopurinol groups (P < 0.05). Apoptosis index was also decreased in groups 2 and 3, compared to group 1 (P < 0.05). There was no significant difference in average TGF-β levels between study groups. These findings suggest that administration of vitamin E or allopurinol following the onset of fever can reduce renal damage in pyelonephritis.

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Abbreviations

APN:

acute pyelonephritis

E. coli :

Escherichia coli

UTI:

urinary tract infection

EIA:

enzyme immunosorbent assay

TGF-β:

transforming growth factor-beta

TUNEL:

terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick-end labeling

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Acknowledgment

Special thanks to Drs. Rostambeigy, Vejdani, Elmi, Rahbar, and Salmasi, and to Miss Aligholy and Mr. Farzaneh, for their kind help.

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Authors and Affiliations

  1. Pediatric Urology Research Center, Department of Urology, Children’s Hospital Medical Center, Tehran University/Medical Sciences, Tehran, Iran

    Zhina Sadeghi, Abdol-Mohammad Kajbafzadeh, Seyedmehdi Payabvash & Azadeh Elmi

  2. Department of Epidemiology & Biostatistics, School of Public Health, Tehran University/Medical Sciences, Tehran, Iran

    Parvin Tajik

  3. Department of Pathology, Children’s Hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran

    Maryam Monajemzadeh

  4. No. 36, 2nd Floor, 7th Street, Saadat-Abad Ave, Tehran, 19987, Iran

    Abdol-Mohammad Kajbafzadeh

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  1. Zhina Sadeghi
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  2. Abdol-Mohammad Kajbafzadeh
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  5. Seyedmehdi Payabvash
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Correspondence to Abdol-Mohammad Kajbafzadeh.

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Sadeghi, Z., Kajbafzadeh, AM., Tajik, P. et al. Vitamin E administration at the onset of fever prevents renal scarring in acute pyelonephritis. Pediatr Nephrol 23, 1503–1510 (2008). https://doi.org/10.1007/s00467-008-0853-7

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  • DOI: https://doi.org/10.1007/s00467-008-0853-7

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