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MAPK and angiotensin II receptor in kidney of newborn rats from losartan-treated dams

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Abstract

Several lines of evidence suggest that angiotensin II (A-II) participates in the postnatal development of the kidney in rats. Many effects of A-II are mediated by mitogen-activated protein kinase (MAPK) pathways. This study investigated the influence that treatment with losartan during lactation has on MAPKs and on A-II receptor types 1 (AT1) and 2 (AT2) expression in the renal cortices of the offspring of dams exposed to losartan during lactation. In addition, we evaluated the relationship between such expression and changes in renal function and structure. Rat pups from dams receiving 2% sucrose or losartan diluted in 2% sucrose (40 mg/dl) during lactation were killed 30 days after birth, and the kidneys were removed for histological, immunohistochemical, and Western blot analysis. AT1 and AT2 receptors and p-p38, c-Jun N-terminal kinases (p-JNK) and extracellular signal-regulated protein kinases (p-ERK) expression were evaluated using Western blot analysis. The study-group rats presented an increase in AT2 receptor and MAPK expression. In addition, these rats also presented lower glomerular filtration rate (GFR), greater albuminuria, and changes in renal structure. In conclusion, newborn rats from dams exposed to losartan during lactation presented changes in renal structure and function, which were associated with AT2 receptor and MAPK expression in the kidneys.

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Acknowledgements

Financial support was provided in the form of a grant from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Foundation for the Support of Research in the state of São Paulo). The authors are grateful to Cleonice G. A. da Silva, Adriana L. G. de Almeida, and Erica Delloiagono for technical assistance.

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Authors and Affiliations

  1. Department of Physiology, University of São Paulo School of Medicine, Av. Bandeirantes, 3900, 14049-900, Ribeirão Preto, São Paulo, Brazil

    Ana Paula Coelho Balbi, Evelyn Cristina Santana Marin, Heloisa Della Coletta Francescato & Terezila Machado Coimbra

  2. Department of Pathology, University of São Paulo School of Medicine, São Paulo, Brazil

    Roberto Silva Costa

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  1. Ana Paula Coelho Balbi
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  2. Evelyn Cristina Santana Marin
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  5. Terezila Machado Coimbra
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Correspondence to Terezila Machado Coimbra.

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Balbi, A.P.C., Marin, E.C.S., Francescato, H.D.C. et al. MAPK and angiotensin II receptor in kidney of newborn rats from losartan-treated dams. Pediatr Nephrol 23, 1433–1444 (2008). https://doi.org/10.1007/s00467-008-0830-1

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