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Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis

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Abstract

Recent studies have suggested that gene expression studies using urinary sediment might be a non-invasive approach to assessing activity and pathogenesis in glomerulonephritis. However, little information is available regarding the mRNA expression patterns of functional molecules, such as T-bet, GATA-3, FOXP3, and retinoic acid-inducible gene-I (RIG-I), in urinary sediment, from patients with immunocomplex-mediated glomerulonephritis. Fourteen lupus nephritis (LN) patients, 13 IgA nephropathy (IgAN) patients, and 12 healthy controls were enrolled in the study. The mRNA expressions of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment were measured using real time quantitative polymerase chain reaction. We also studied the expression of RIG-I in kidney tissue specimens obtained from LN and IgAN patients. Significant differences in the expression patterns of GATA-3, FOXP3 and RIG-I, and marginal differences in T-bet expression, were observed between the three study groups. Immunofluorescent staining for RIG-I was observed in the tissue specimens from the LN patients, but not in those from the IgAN patients. The mRNA expression patterns of T-bet, GATA-3, FOXP3 and RIG-I in urinary sediment differ according to diagnostic category. These results suggest that the measurement of these target gene expressions might be a useful, non-invasive method for clinical monitoring and studying of pathogenesis in glomerulonephritis.

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Acknowledgment

The authors would like to thank Professor T. Miyawaki, Department of Pediatrics, Toyama University School of Medicine, and Professor T. Oite, Department of Cellular Physiology, Institute of Nephrology, Graduate School of Medical and Dental Sciences, Niigata University, for valuable discussions.

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Authors and Affiliations

  1. Department of Pediatrics, Hirosaki University School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Japan

    Koji Tsugawa, Eishin Oki, Koichi Suzuki, Etsuro Ito & Hiroshi Tanaka

  2. Department of Vascular Biology, Hirosaki University School of Medicine, Hirosaki, Japan

    Tadaatsu Imaizumi

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  1. Koji Tsugawa
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  2. Eishin Oki
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  3. Koichi Suzuki
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  4. Tadaatsu Imaizumi
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  5. Etsuro Ito
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  6. Hiroshi Tanaka
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Correspondence to Koji Tsugawa.

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Tsugawa, K., Oki, E., Suzuki, K. et al. Expression of mRNA for functional molecules in urinary sediment in glomerulonephritis. Pediatr Nephrol 23, 395–401 (2008). https://doi.org/10.1007/s00467-007-0683-z

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  • DOI: https://doi.org/10.1007/s00467-007-0683-z

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