Skip to main content

Advertisement

SpringerLink
Log in

Effect of selenium-deficient diet on tubular epithelium in normal rats

  • Original Article
  • Published:
Pediatric Nephrology Aims and scope Submit manuscript

Abstract

Selenium (Se) deficiency reduces glutathione peroxidase (GPx) activity, resulting in increased oxidative stress. We examined how Se deficiency induces renal injury via oxidative stress over time during the Se-deficient period. Seventy-two male Wistar rats were divided into two groups and fed either a control or Se-deficient diet. Rats were sacrificed on weeks 1, 2, 4, 6, 9, and 12. Blood and urine samples were collected, and the kidneys were removed. Urinalysis was performed, and creatinine clearance (Ccr) was calculated. Expressions of cellular GPx (cGPx) and phospholipid hydroperoxidase GPx (PHGPx) mRNA and GPx activity were measured. Histology was evaluated by light microscopy with immunohistochemistry for 4-hydroxy-2-nonenal (HNE) and vimentin. The Se-deficient diet caused significant decreases in GPx activity and cGPx mRNA expression but no change in PHGPx mRNA, together with significant proteinuria and glucosuria and slight decline in Ccr. The Se-deficient diet induced calcification in the kidney and increased the distribution of HNE and vimentin immunostaining in proximal tubuli, particularly around the outer medulla stripe. However, the histological damage did not progress after 6 weeks of deficiency. Se deficiency induces proteinuria and glucosuria with renal calcification, which may be primarily induced by injury of proximal tubuli via oxidative stress.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Baliga R, Baliga M, Shah SV (1992) Effect of selenium-deficient diet in experimental glomerular disease. Am J Physiol 263:F56–F61

    CAS  PubMed  Google Scholar 

  2. Nath KA, Salahudeen AK (1990) Induction of renal growth and injury in the intact rat kidney by dietary deficiency of antioxidants. J Clin Invest 86:1179–1192

    Article  CAS  Google Scholar 

  3. Reddi AS, Bollineni JS (2001) Selenium-deficient diet induces renal oxidative stress and injury via TGF-β 1 in normal and diabetic rats. Kidney Int 59:1342–1353

    Article  CAS  Google Scholar 

  4. Alftham G, Kumpulainen J (1982) Determination of selenium in small volume of blood plasma and serum by electrothermal atomic absorption spectrometry. Ann Chim Acta 140:221–227

    Article  Google Scholar 

  5. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  6. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  PubMed  Google Scholar 

  7. L’Abbe MR, Trick KD (1994) Changes in pancreatic glutathione peroxidase and superoxide dismutase activities in the prediabetic diabetes-prone BB rat. Proc Soc Exp Biol Med 207:206–212

    Article  Google Scholar 

  8. Imai H, Nakagawa Y (2003) Biological significance of phospholipids hydroperoxide glutathione peroxidase (PHGPx, GPX4) in mammalian cells. Free Radic Biol Med 34:145–169

    Article  CAS  Google Scholar 

  9. Chen MM, Lam A, Abraham JA, Schreiner GF, Joly AH (2000) CTGF expression is induced by TGF-β in cardiac fibroblasts and cardiac myocytes: a potential role in heart fibrosis. J Mol Cell Cardiol 32:1805–1819

    Article  CAS  Google Scholar 

  10. Bermano G, Nicol F, Dyer JA, Sunde RA, Beckett GJ, Arthur JR, Hesketh JE (1995) Tissue-specific regulation of selenoenzyme gene expression during selenium deficiency in rats. Biochem J 311:425–430

    Article  CAS  Google Scholar 

  11. Nakane T, Asayama K, Kodera K, Hayashide H, Uchida N, Nakazawa S (1998) Effect of selenium deficiency on cellular and extracellular glutathione peroxidase: immunochemical detection and mRNA analysis in rat kidney and serum. Free Radial Biol Med 25:504–511

    Article  CAS  Google Scholar 

  12. Pedraza-Chaverri J, Arevalo AE, Hernandez-Pando R, Larriva-Sahd J (1995) Effect of dietry antioxidants on puromycin aminonucleoside nephritic syndrome. Int J Biochem Cell Biol 27:683–691

    Article  Google Scholar 

  13. Mylona-Karayanni C, Gourgiotis D, Bossios A, Kamper EF (2006) Oxidative stress and adhesion molecules in children with type 1 diabetes mellitus: a possible link. Pediatr Diabetes 7:51–59

    Article  Google Scholar 

  14. Muthukumar A, Selvam R (1997) Renal injury mediated calcium oxalate nephrolithiasis: role of lipid peroxidation. Ren Fail 19:401–408

    Article  CAS  Google Scholar 

  15. Khan SR, Hackett RL (1993) Hyperoxaluria, enzymuria and nephrolithiasis. Contrib Nephrol 101:190–193

    Article  CAS  Google Scholar 

  16. Fukuda A, Osawa T, Hitomi K, Uchida K (1996) 4-hydroxy-2-nonenal cytotoxicity in renal proximal tubular cells. Protein modification and redox alteration. Arch Biochem Biophys 333:419–426

    Article  CAS  Google Scholar 

  17. Bartsch H, Nair J, Velic I (1997) Etheno-DNA base adducts as tools in human cancer aetiology and chemoprevention. Eur J Cancer Prev 6:529–534

    Article  CAS  Google Scholar 

  18. Toyokuni S, Miyake N, Hirai H, Hagiwara M, Kawakishi S, Osawa T, Uchida K (1995) The monoclonal antibody specific for the 4-hydroxy-2-nonenal histidine adducts. FEBS Lett 359:189–191

    Article  CAS  Google Scholar 

  19. Tolstrong GV, Shoeman RL, Traub U, Traub P (2001) Role of intermediate filament protein vimentin in delaying senescence and in the spontaneous immortalization of mouse embryo fibroblasts. DNA Cell Biol 20:509–529

    Article  Google Scholar 

  20. Belichenko I, Morishima N, Separovic D (2001) Caspase-resistant vimentin suppresses apoptosis after photodynamic treatment with a silicon phthalocyanine in Jurkat cells. Arch Biochem Biophys 390:57–63

    Article  CAS  Google Scholar 

  21. Frei B, Winterhalter KH, Richter C (1985) Mechanism of alloxan-induced calcium release from rat liver mitochondria. J Biol Chem 260:7394–7401

    CAS  PubMed  Google Scholar 

  22. Isaka Y, Fujiwara Y, Ueda N, Kanea Y, Kamada T, Imai E (1993) Glomerulosclerosis induced by in vitro transfection of transforming growth factor-β or platelet-derived growth factor gene into the rat kidney. J Clin Invest 92:2597–2601

    Article  CAS  Google Scholar 

  23. Kopp JB, Factor VM, Mozes M, Nagy P, Sanderson N, Bottinger EP, Klotman PE, Thorgeirsson SS (1996) Transgenic mice with increased plasma levels of TGF-β 1 develop progressive renal disease. Lab Invest 74:991–1003

    CAS  PubMed  Google Scholar 

  24. Roberts AB, McCune BK, Sporn MB (1992) TGF-β: regulation of extracellular matrix. Kidney Int 41:557–559

    Article  CAS  Google Scholar 

  25. Basile DP (1999) The transforming growth factor beta system in kidney disease and repair: recent progress and future directions. Curr Opin Nephrol Hypertens 8:21–30

    Article  CAS  Google Scholar 

  26. Maiorino M, Thomas JP, Girotti AW, Ursini F (1991) Reactivity of phospholipid hydroperoxide glutathione peroxidase with membrane and lipoprotein lipid hydroperoxides. Free Radic Res Commun 12–13:131–135

    Article  Google Scholar 

  27. Thomas JP, Maiorino M, Ursini F, Girotti AW (1990) Protective action of phospholipids hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation. In situ reduction of phospholipids and cholesterol hydroperoxides. J Biol Chem 265:454–461

    CAS  PubMed  Google Scholar 

  28. Alissa EM, Bahijri SM, Ferns GA (2003) The controversy surrounding selenium and cardiovascular disease: a review of the evidence. Med Sci Monit 9:9–18

    Google Scholar 

  29. Becker DJ, Reul B, Ozcelikay AT, Buchet JP, Henquin J-C, Brichard SM (1996) Oral selenate improves glucose homeostasis and partly reverse abnormal expression of liver glycolytic and gluconeogenic enzymes in diabetic rats. Diabetologia 39:3–11

    Article  CAS  Google Scholar 

  30. Battell ML, Delgatty HLM, McNeill JH (1998) Sodium selenate corrects glucose tolerance and heart function in STZ diabetic rats. Mol Cell Biochem 179:27–34

    Article  CAS  Google Scholar 

  31. Danda RS, Habiba NM, Rincon-Choles H, Bhandari BK, Barnes JL, Abboud HE, Pergola PE (2005) Kidney involvement in a nongenetic rat model of type 2 diabetes. Kidney Int 68:2562–2571

    Article  CAS  Google Scholar 

  32. Nath KA, Grande J, Croatt A, Haugen J, Kim Y, Rusenberg ME (1998) Redox regulation of renal DNA synthesis, transforming growth factor-β 1 and collagen gene expression. Kidney Int 53:367–381

    Article  CAS  Google Scholar 

  33. Deguchi T, Takamoto M, Uehara N, Lidup WE, Suenaga A, Otagiri M (2005) Renal clearance of endogenous hippurate correlates with expression levels of renal organic anion transporters in uremic rats. J Pharmacol Exp Ther 314:932–938

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pediatrics, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan

    Mikiya Fujieda, Keishi Naruse, Tadashi Hamauzu & Hiroshi Wakiguchi

  2. First Department of Pathology, Kochi Medical School, Kochi University, Kochi, Japan

    Keishi Naruse, Tadashi Hamauzu, Eriko Miyazaki, Yoshihiro Hayashi & Hideaki Enza

  3. Department of Pharmacology, Kobe Gakuin University, Kobe, Japan

    Riyo Enomoto & Eibai Lee

  4. Department of Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan

    Kazuhide Ohta

  5. Department of Pathology, Kashiwa Hospital, The Jikei University, School of Medicine, Chiba, Japan

    Yutaka Yamaguchi

Authors
  1. Mikiya Fujieda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Keishi Naruse
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tadashi Hamauzu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eriko Miyazaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yoshihiro Hayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Riyo Enomoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Eibai Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kazuhide Ohta
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Yutaka Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Hiroshi Wakiguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Hideaki Enza
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mikiya Fujieda.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fujieda, M., Naruse, K., Hamauzu, T. et al. Effect of selenium-deficient diet on tubular epithelium in normal rats. Pediatr Nephrol 22, 192–201 (2007). https://doi.org/10.1007/s00467-006-0266-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00467-006-0266-4

Keywords

Search

Navigation