Abstract
Uninephrectomy (Unx) is followed by the compensatory renal growth (CRG) of the remaining kidney. Previous evidence has shown that during CRG, renal tissue is resistant to a variety of pathologies. We tested the hypothesis that the functional changes that take place during CRG could attenuate Shiga toxin (Stx) toxicity in a mouse model of Stx2-induced hemolytic uremic syndrome (HUS). The participation of nitric oxide (NO) was analyzed. After CRG induction with Unx, mice were exposed to a lethal dose of Stx2, and the degree of renal damage and mortality was measured. Stx2 effects on the growth, renal blood flow (RBF) and NO synthase (NOS) intrarenal expression in the remaining kidney were then studied. The induction of CRG strongly prevented Stx2-mediated mortality and renal damage. Administration of the NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME) during CRG partially impaired the protection. Both Stx2 and L-NAME interfered with the hypertrophic and hyperplastic responses to Unx, as well as with the increase in RBF. In intact mice, Stx2 decreased renal perfusion, inhibited endothelial NOS basal expression and enhanced inducible NOS expression; all of these effects were attenuated by prior Unx. It is concluded that during CRG mice are highly protected against Stx2 toxicity and lethality. The protective capacity of CRG could be related to the enhancement of renal perfusion and preservation of eNOS renal expression, counterbalancing two major pathogenic mechanisms of Stx2.
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References
Fine L (1986) The biology of renal hypertrophy. Kidney Int 29:619–634
Wolf G, Neilson EG (1991) Molecular mechanisms of tubulointerstitial hypertrophy and hyperplasia. Kidney Int 39:401–420
Johnson H, Vera Roman JM (1966) Compensatory renal enlargement. Am J Pathol 49:1–13
Lopez-Novoa JM, Ramos B, Martín-Oar JE, Hernando L (1982) Functional compensatory changes after unilateral nephrectomy in rats. General and intrarenal hemodynamic alterations. Renal Physiol 5:76–84
Ring K, Benson M, Bandyk M, Sawczuk I (1992) Detection of cellular proliferation during compensatory renal growth using flow cytometry. Nephron 61:200–203
Ramos B, López-Novoa JM, Hernando L (1982) Role of hemodynamic alterations in the partial protection afforded by uninephrectomy against glycerol-induced acute renal failure in rats. Nephron 30:68–72
Fried TA, Hishida A, Barnes JL, Stein JH (1984) Ischemic acute renal failure in the rat: Protective effect of uninephrectomy. Am J Physiol 247:568–574
Nakajima T, Miyaji T, Kato A, Ikegaya N, Yamamoto T, Hishida A (1996) Uninephrectomy reduces apoptotic cell death and enhances renal tubular regeneration in ischemic ARF in rats. Am J Physiol 271:846–853
Kato A, Hishida A, Nakajima T (1995) Role of thromboxane A2 and prostacyclin in uninephrectomy-induced attenuation of ischemic renal injury. Kidney Int 48:1577–1583
Kato A, Hishida A, Tanaka I, Komatsu K (1997) Uninephrectomy prevents the ischemia induced increase in renin activity. Nephron 75:72–76
Kato A, Hishida A (2001) Amelioration of post-ischaemic renal injury by contralateral uninephrectomy: a role of endothelin-1. Nephrol Dial Transplant 16:1570–1576
Ray PE, Liu XH (2001) Pathogenesis of Shiga toxin-induced hemolytic uremic syndrome. Pediatr Nephrol 16:823–839
Obrig TG (1997) Shiga toxin mode of action in E. coli O157:H7 disease. Front Biosci 2:635–642
Karmali MA (2004) Infection by Shiga toxin-producing Escherichia coli: an overview. Mol Biotechnol 26:117–122
Cotran RS, Kumar V, Collins T (1999) Robbin’s pathologic basis of disease. WB Saunders, Philadelphia, PA, pp 969
Fernandez GC, Te Loo MW, van der Velden TJ, van der Heuvel LP, Palermo MS, Monnens LL (2003) Decrease in thrombomodulin contributes to the procoagulant state of endothelium in hemolytic uremic syndrome. Pediatr Nephrol 18:1066–1068
Te Loo M, Bosma N, Van Hinsbergh V, Span P, De Waal R, Clarijs R, Sweep C, Monnens L, van der Heuvel L (2004) Elevated levels of vascular endothelial growth factor in serum of patients with D-HUS. Pediatr Nephrol 19:754–760
Moncada S, Palmer RM, Higgs EA (1991) Nitric oxide: Physiology, pathophysiology and pharmacology. Pharmacol Rev 43:109–142
Morris SM, Billiar TR (1994) New insights into the regulation of inducible nitric oxide synthesis. Am J Physiol 266:829–839
Noris M, Ruggenenti P, Todeschini M, Figliuzzi M, Macconi D, Zoja C, Paris S, Gaspari F, Remuzzi G (1996) Increased nitric oxide formation in recurrent thrombotic microangiopathies: a possible mediator of microvascular injury. Am J Kidney Dis 27:790–796
Dran G, Fernández GC, Rubel CJ, Bermejo E, Gomez S, Isturiz MA, Palermo M (2002) Participation of L-arginine-nitric oxide pathway in the pathogenesis of hemolytic uremic syndrome in a murine model. Kidney Int 62:1338–1348
Valdivielso JM, Perez-Barriocanal F, García-Estan J, López-Novoa JM (1999) Role of nitric oxide in the early renal hemodynamic response after unilateral nephrectomy. Am J Physiol 276:1718–1723
Sigmon DH, Gonzalez-Feldman E, Cavasin MA, Potter DL, Beierwaltes WH (2004) Role of nitric oxide in the renal hemodynamic response to unilateral nephrectomy. J Am Soc Nephrol 15:1413–1420
Tolins JP, Palmer RM, Moncada S, Raij L (1990) Role of endothelium-derived relaxing factor in regulation of renal hemodynamic responses. Am J Physiol 258:655–662
Raij L, Baylis C (1995) Glomerular actions of nitric oxide. Kidney Int 48:20–32
Perico N, Remuzzi G (2002) Nitric oxide and renal perfusion in humans. J Hypertens 20:391–393
Jansen A, Cook T, Michael T, Largen P, Riveros MV, Moncada S, Cattell V (1994) Induction of nitric oxide synthase in rat immune complex glomerulonephritis. Kidney Int 45:1215–1219
Furusu A, Miyasaki M, Abe K, Tsukasaki S, Shioshita K, Sasaki O, Miyasaki K, Ozono Y, Koji T, Harada T, Sakai H, Kohno S (1998) Expression of endothelial and inducible nitric oxide synthase in human glomerulonephritis. Kidney Int 53:1760–1768
Zhou XI, Laszik Z, Ni Z, Wang XQ, Brackett DJ, Lerner MR, Silva FG, Vaziri ND (2000) Down regulation of renal endothelial nitric oxide synthase expression in experimental glomerular thrombotic microangiopathy. Lab Invest 80:1079–1087
Palermo MS, Alves Rosa MF, Rubel C, Fernández GC, Fernández Alonso G, Alberto F, Rivas M, Isturiz MA (2000) Pretreatment of mice with lipopolysaccharide (LPS) or IL1β exerts dose-dependent opposite effects on Shiga toxin-2 lethality. Clin Exp Immunol 119:77–83
National Institutes of Health (NIH) (1985) Guide for the care and use of laboratory animals. Government Printing Office, Washington, DC
Orucevic A, Lala PK (1996) N-nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthesis, ameliorates interleukin 2-induced capillary leakage and reduces tumor growth in adenocarcinoma-bearing mice. Br J Cancer 73:189–196
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Kasina S, Fritzberg AR, Johnson DL, Eshima D (1986) Tissue distribution properties of technetium-99m-diamide-dimercaptide complexes and potential use as a renal radiopharmaceutical. J Med Chem 29:1933–1940
Ercan MT, Gulaldi NC, Unsal IS, Aydin M, Peksoy I, Hascelik Z (1996) Evaluation of Tc-99m (V) DMSA for imaging inflammatory lesions: an experimental study. Ann Nucl Med 10:419–423
Rosignoli F, Goren N, Perez Leirós C (1986) Alterations in nitric oxide synthase activity and expression in submandibular glands of NOD mice. Clin Immunol 101:86–93
Ikeda M, Ito S, Honda M (2004) Hemolytic uremic syndrome induced by lipopolysaccharide and Shiga-like toxin. Pediatr Nephrol 19:485–489
Obrig TG, Del Vecchio PJ, Brown JE, Moran TP, Rowland BM, Judge TK, Rothman SW (1988) Direct cytotoxic action of Shiga toxin on human vascular endothelial cells. Infect Immun 56:2373–2378
Majoul I, Schmidt T, Pomasanova M, Boutkevich E, Kozlov Y, Soling HD (2002) Differential expression of receptors for Shiga and Cholera toxins is regulated by the cell cycle. J Cell Sci 115:817–826
Scholbach TM (1986) Changes of renal flow volume in the hemolytic uremic syndrome—Color Doppler sonographic investigations. Pedriatr Nephrol 16:644–647
West JB (1991) Physiological basis of medical practice, 12th edn. Waverly, Baltimore, MD, pp 530
Barger AC, Herd JA (1971) The renal circulation. N Engl J Med 284:482–490
Karlberg L, Norlen BJ, Ojteg G, Wolgast M (1983) Impaired medullary circulation in postischemic acute renal failure. Acta Physiol Scand 118:11–17
Brezis MS, Rosen S, Silva RP, Epstein FH (1984) Renal ischemia. A new perspective. Kidney Int 26:375–383
Schwartz D, Mendonca M, Schwartz I, Xia Y, Satriano J, Wilson CB, Blantz RC (1997) Inhibition of constitutive nitric oxide synthase (NOS) by nitric oxide generated by inducible NOS after lipopolysaccharide administration provokes renal dysfunction in rats. J Clin Invest 100:439–448
Williams JM, Lote CJ, Thewels A, Wood JA, Howie AJ, Williams DA, Taylor M (2000) Role of nitric oxide in a toxin-induced model of haemolytic uremic syndrome. Pediatr Nephrol 14:1066–1070
Aka JA, Jelacic S, Ciol MA, Watkins SL, Murray KF, Christie DL, Klein EJ, Tarr PI (2005) Relative nephroprotection during Escherichia coli O157:H7 infections: association with intravenous volume expansion. Pediatrics 115:e673–e680
Acknowledgements
We thank Dr. Carlos Amorena, University of San Martin Bs. As., for his critical review of the manuscript, and Vet. Hector Costa for excellent technical assistance. This work was supported by grants from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Fundación “Alberto J. Roemmers” and Agencia Nacional de Promoción Científica y Tecnológica, Argentina.
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Camerano, G.V., Bustuoabad, O.D., Meiss, R.P. et al. Compensatory renal growth protects mice against Shiga toxin 2-induced toxicity. Pediatr Nephrol 21, 1082–1092 (2006). https://doi.org/10.1007/s00467-006-0115-5
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DOI: https://doi.org/10.1007/s00467-006-0115-5