Abstract
Viruses have been suspected to be one of the causes of IgA nephropathy (IgAN). Recent studies have detected viruses in renal tissues of patients with IgAN. Enteroviruses have been reported as pathogenic agents in some renal diseases. We previously reported that group B coxsackieviruses cause pathological changes in experimentally infected mouse kidney. The aim of the present study was to examine the participation of enteroviruses in the pathogenesis of renal diseases including IgAN. Renal biopsies of ten patients with IgAN (group 1) and of 19 patients with non-IgAN renal disease(group 2) were analyzed by polymerase chain reaction (PCR) for the presence of enteroviral RNA. Positive PCR results were obtained for three patients (30%) of group 1. We confirmed by sequencing that the positive PCR products were derived from strains of enteroviruses. One of these three patients also had a positive result for lymphocytes from peripheral blood. In contrast, enteroviral RNA was detected in none of the 19 patients of group 2. The incidence of enteroviral RNA detection in patients of group 1 was higher than that in group 2 (P<0.05). Our findings suggest that enteroviral infection may have the possibility of becoming one of the factors involved in the mechanism of onset or evolution of IgAN.
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Tomino Y, Yagame M, Omata F, Nomoto Y, Sakai H (1987) A case of IgA nephropathy associated with adeno- and herpes simplex viruses. Nephron 47:258–261
Gregory M, Hammond ME, Brewer ED (1988) Renal deposition of cytomegalovirus antigen in immunoglobulin A nephropathy. Lancet 2:11–14
Müller GA, Müller CA, Blum GE, Kühn W, Risler T, Bohle A, Lipkovski JM (1992) Human cytomegalovirus in immunoglobulin A nephropathy: detection by polymerase chain reaction. Nephron 62:389–393
Sinniah R, Khan TN, Dodd S (1993) An in situ hybridization study of herpes simplex and Epstein Barr viruses in IgA nephropathy and non-immune glomerulonephritis. Clin Nephrol 40:137–141
Iwama H, Horikoshi S, Shirato I, Tomino Y (1998) Epstein–Barr virus detection in kidney biopsy specimens correlates with glomerular mesangial injury. Am J Kidney Dis 32:785–793
Grist NR, Bell EJ, Assaad F (1978) Enteroviruses in human disease. Prog Med Virol 24:114–157
Burch GE, Colcolough HL (1969) Progressive Coxsackie viral pancarditis and nephritis. Ann Intern Med 71:963–970
Burch GE, Chu KC, Colocolough HL, Sohal RS (1969) Immunofluorescent localization of Coxsackie B antigen in the kidney observed at routine autopsy. Am J Med 47:36–42
Austin TW, Ray CG (1973) Coxsackie virus group B infections and the hemolytic uremic syndrome. J Infect Dis 127:698–701
Aronson MD, Phillips CA (1975) Coxsackievirus B5 infections in acute oliguric renal failure. J Infect Dis 132:303–306
Sun SH, Burch GE, Sohal RS, Chu KC (1967) Coxsackie B4 viral nephritis in mice and its autoimmune-like phenomena. Proc Soc Exp Biol Med 126:882–885
Mutoh S, Kumada K, Kume K, Suzuki J, Yokota T, Suzuki H (1994) Detection of coxsackie B4 virus RNA in infected mouse kidneys by in situ hybridization. Nephron 67:340–345
Yoshida K, Suzuki S, Kume K, Mutoh S, Suzuki J, Kato K, Suzuki H (1997) Experimental IgA nephropathy induced by Coxsackie B4 virus in mice. Am J Nephrol 17:81–88
Isome M, Yoshida K, Suzuki S, Kume K, Suzuki J, Kato K, Suzuki H (1997) Experimental glomerulonephritis following successive inoculation of five different serotypes of group B coxsackieviruses in mice. Nephron 77:93–99
Hosoya M, Honzumi K, Suzuki H (1997) Detection of enterovirus by polymerase chain reaction and culture in cerebrospinal fluid of children with transient neurologic complications associated with acute febrile illness. J Infect Dis 175:700–703
Hosoya M, Honzumi K, Sato M, Katayose M, Kato K, Suzuki H (1998) Application of PCR for various neurotropic viruses on the diagnosis of viral meningitis. J Clin Virol 11:117–124
Zoll GJ, Melchers WJG, Kopecka H, Jambroes G, Poel HJA, Galama JMD (1992) General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections. J Clin Microbiol 30:160–165
Churg J, Bernstein J, Glassock RJ (1995) Renal disease. Classification and atlas of glomerular diseases (edn 2). Igaku-shoin, Tokyo, Japan
Southwest Pediatric Nephrology Study Group (1985) A clinico-pathologic study of crescentic glomerulonephritis in 50 children: a report of the Southwest Pediatric Nephrology Study Group. Kidney Int 27:450–458
Conaldi PG, Biancone L, Bottelli A, De Martino A, Camussi G, Toniolo A (1997) Distinct pathogenic effects of group B coxsackieviruses on human glomerular and tubular kidney cells. J Virol 71:9180–9187
Matteucci D, Paglianti M, Giangregorio AM, Capobianchi MR, Dianzani F, Bendinelli M (1985) Group B coxsackieviruses readily establish persistent infections in human lymphoid cell lines. J Virol 56:651–654
Schnurr DP, Schmidt NJ (1984) Persistent infection of mouse fibroblasts with coxsackievirus. Arch Virol 81:91–101
Frank JA, Schmidt EV, Smith RE, Wilfert CM (1986) Persistent infection of rat insulinoma cells with coxsackie B4 virus. Acta Virol 87:143–150
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Takahashi, A., Kawasaki, Y., Yoshida, K. et al. Detection of enteroviruses in renal biopsies from patients with immunoglobulin A nephropathy. Pediatr Nephrol 20, 1578–1582 (2005). https://doi.org/10.1007/s00467-005-2019-1
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DOI: https://doi.org/10.1007/s00467-005-2019-1