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Postnatal renal development of rats from mothers that received increased sodium intake

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Abstract

The newborn rat kidney is not fully developed until approximately 12 days after birth. Several lines of evidence suggest that angiotensin II (AII) participates in the postnatal development of the kidney. The aim of the present study was to analyze proliferating cell nuclear antigen (PCNA), fibronectin, α-smooth muscle-actin (α-SM-actin), and AII expression in renal cortex during development in rats born to mothers that received a normal (control) or increased (experimental) sodium intake during pregnancy. Ninety Wistar rats aged 1, 7, 15, and 30 days from the control and experimental groups were killed and the kidneys removed for histological and immunohistochemical studies. The results showed higher fibronectin, α-SM-actin, PCNA, and AII expression in the glomerular and tubulointerstitial areas of the renal cortex of 1- and 7-day-old animals, which decreased with renal development. The animals from the experimental group showed at 1 day of age a decrease in α-SM-actin, fibronectin, PCNA, and AII expression compared with controls of the same age ( P <0.05). In conclusion, our data show that increased sodium intake during pregnancy induces a reduction of α-SM-actin, fibronectin, and PCNA expression in the renal cortex tubulointerstitium and glomeruli of neonatal rats. These alterations may be related to the decrease of AII expression also observed in the renal cortex from these animals.

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Acknowledgements

The authors thank Cleonice G.A. da Silva, Erika Delloiagono, and Rubens Fernando de Melo for expert technical assistance. Ana Paula C. Balbi was a recipient of Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, DF, Brazil, fellowship, and Dr. Roberto Silva Costa and Dr. Terezila Machado Coimbra are recipients of Conselho Nacional de Desenvolvimento Científico e Tecnológico, DF, Brazil, fellowships. These results were presented in abstract form at the American Society of Nephrology Meeting, Philadelphia, Pa., November 2002.

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Authors and Affiliations

  1. Department of Physiology, Faculty of Medicine, University of Sao Paulo, Avenue Dos Bandeirantes 3900, Ribeirao Preto, CEP-14049-900, Sao Paulo, Brazil

    Ana Paula C. Balbi & Terezila M. Coimbra

  2. Department of Pathology, Faculty of Medicine, University of Sao Paulo, Avenue Dos Bandeirantes 3900, Ribeirao Preto, CEP-14049-900, Sao Paulo, Brazil

    Roberto S. Costa

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  1. Ana Paula C. Balbi
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  3. Terezila M. Coimbra
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Correspondence to Terezila M. Coimbra.

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Balbi, A.P.C., Costa, R.S. & Coimbra, T.M. Postnatal renal development of rats from mothers that received increased sodium intake. Pediatr Nephrol 19, 1212–1218 (2004). https://doi.org/10.1007/s00467-004-1586-x

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  • DOI: https://doi.org/10.1007/s00467-004-1586-x

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