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Oxidative stress in a rat model of nephrosis can be quantified by electron spin resonance

Pediatric Nephrology volume 19, pages 266–270 (2004)Cite this article

Abstract

The pathogenesis of nephrotic syndrome is not clear. In this study, we used electron spin resonance (ESR) to evaluate levels of reactive oxygen species in rats with puromycin aminonucleoside (PAN)-induced nephrosis. Twenty-six Wistar rats were divided into four groups: (1) PAN treated, (2) PAN treated and α-tocopherol supplemented, (3) supplemented with α-tocopherol only, (4) control. On day 9, urinary protein excretion was measured. On day 10, all animals were sacrificed with retrograde perfusion via the aorta to obtain renal venous perfusates. The signal intensities of ascorbate radicals in the perfusates were determined by ESR. After perfusion, the kidneys were isolated and sieved to obtain glomeruli for determination of glomerular thiobarbituric acid-reactive substance (TBArs) and α-tocopherol. Urinary protein excretion by PAN-treated rats increased significantly on day 9 and was reduced by α-tocopherol supplementation. The ascorbate radical intensity and glomerular TBArs level were higher in PAN-treated than in control rats and were both suppressed to control levels by α-tocopherol supplementation. There were positive correlations between ascorbate radical intensity and the daily urinary protein, as well as between ascorbate radical intensity and the glomerular TBArs level. Hence, it is possible to quantify oxidative stress due to PAN nephrosis by ESR. Our findings suggest that lipid peroxidation plays an important role in the pathogenesis of proteinuria in PAN-treated rats.

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Acknowledgements

This work was supported by a Grant-in-Aid (12770614) for Scientific Research from the Ministry of Education, Science, and Culture, Japan, and a Grant from the High Technique Research Center, Osaka Medical College.

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  1. Department of Pediatrics, Osaka Medical College, 2–7 Daigakumachi, Takatsuki-shi, 569–8686, Osaka, Japan

    Hyogo Nakakura, Akira Ashida, Kazuya Hirano & Hiroshi Tamai

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  1. Hyogo Nakakura
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  2. Akira Ashida
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  3. Kazuya Hirano
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  4. Hiroshi Tamai
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Correspondence to Akira Ashida.

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Nakakura, H., Ashida, A., Hirano, K. et al. Oxidative stress in a rat model of nephrosis can be quantified by electron spin resonance. Pediatr Nephrol 19, 266–270 (2004). https://doi.org/10.1007/s00467-003-1332-9

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  • DOI: https://doi.org/10.1007/s00467-003-1332-9

Keywords

  • Puromycin aminonucleoside
  • Electron spin resonance
  • Reactive oxygen species
  • Thiobarbituric acid-reactive substances
  • α-Tocopherol
  • Nephrotic syndrome