Abstract
Background
Although systemic responses to carbon dioxide (CO2) pneumoperitoneum have been studied, there have been few reports of local responses within the peritoneum. We investigated the expression of mRNA for adhesion molecules involved in cell-cell interactions, including ICAM-1, VCAM-1, CD44, E-cadherin, P-cadherin, and N-cadherin, afte the induction of a CO2 pneumoperitoneum in mice
Methods
Mice were treated with CO2 pneumoperitoneum (4–6 mmHg for 30 min) and then killed after 24 h, 48 h, and 72 h. The peritoneum of the abdominal wall was resected, and total RNA was extracted by the acid guanidinium thiocyanate-phenol-chloroform extraction procedure, cDNA were synthesized by reverse transcription. Expression of the mRNA for each gene was normalized to that of β-actin for semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
Results
The expression of P-cadherin mRNA was significantly increased at 48 h (p=0.007) and returned to the control level by 72 h after CO2 pneumoperitoneum. The expression of CD44 increased gradually, reaching a peak at 48 h and returning to the control value by 72 h after CO2 pneumoperitoneum. Expression of ICAM-1 mRNA was not changed significantly after the application of CO2.
Conclusion
The expression of P-cadherin mRNA in the peritoneum can be induced to repair injuries to mesothelial cells caused by CO2 pneumoperitoneum.
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Online publication: 12 June 2001
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Tahara, K., Fujii, K., Yamaguchi, K. et al. Increased expression of P-cadherin mRNA in the mouse peritoneum after carbon dioxide insufflation. Surg Endosc 15, 946–949 (2001). https://doi.org/10.1007/s004640090114
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DOI: https://doi.org/10.1007/s004640090114