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Silver nanoparticle-coated self-expandable metallic stent suppresses tissue hyperplasia in a rat esophageal model

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Abstract

Background

To evaluate the efficacy of a silver nanoparticle (AgNP)-coated self-expandable metallic stent (SEMS) for suppressing tissue hyperplasia in a rat esophageal model.

Methods

Twenty-four male Sprague-Dawley rats were randomly assigned to four groups. Animals in group A underwent uncoated SEMS placement, whereas animals in groups B, C, and D underwent 6, 12, and 24 mg/mL AgNP-coated SEMS placement, respectively. All animals were euthanized 4 weeks after SEMS placement, and a gross examination and histological analyses were performed.

Results

All rats achieved technical success and survived until the end of the study. The gross examination showed moderate to severe tissue hyperplasia in 5 rats in group A and 2 rats in group B. In contrast, no animals in groups C and D had moderate or severe tissue hyperplasia. The gross examination revealed no complications. The percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, percentage of connective tissue area, inflammatory cell infiltration grade, degree of collagen deposition, and degrees of Ki67, TUNEL, and α-SMA-positive deposition were significantly lower in groups C and D than in group A (all p < 0.05). However, only the percentage of granulation tissue area, number of epithelial layers, thickness of submucosal fibrosis, and percentage of connective tissue area were significantly lower in group B than in group A (all p < 0.05). No histological parameters were significantly different between group D and group C (all p > 0.05).

Conclusion

AgNP-coated SEMSs suppressed tissue hyperplasia in a rat esophageal model.

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Funding

This work was supported by the National Natural Science Fund of China (Grant Nos. 82001937 & 81871468) and the Fundamental Research Funds for the Central Universities (Grant No. 3332018076).

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Author notes

  1. He Zhao and Yan Fu have contributed equally to this work and are co-first authors.

Authors and Affiliations

  1. Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 17 Panjiayuan Nanli, Chaoyang District, Beijing, 100021, China

    He Zhao, Yan Fu, Jiaywei Tsauo, Xiaowu Zhang, Yanqing Zhao, Tao Gong, Jingui Li & Xiao Li

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  1. He Zhao
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  2. Yan Fu
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  3. Jiaywei Tsauo
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Corresponding author

Correspondence to Jiaywei Tsauo.

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Disclosure

He Zhao, Yan Fu, Jiaywei Tsauo, Xiaowu Zhang, Yanqing Zhao, Tao Gong, Jingui Li, and Xiao Li have no conflicts of interest or financial ties to disclose.

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All applicable institutional and/or national guidelines for the care and use of animals were followed.

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Zhao, H., Fu, Y., Tsauo, J. et al. Silver nanoparticle-coated self-expandable metallic stent suppresses tissue hyperplasia in a rat esophageal model. Surg Endosc 36, 66–74 (2022). https://doi.org/10.1007/s00464-020-08238-4

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  • DOI: https://doi.org/10.1007/s00464-020-08238-4

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