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Small bowel in vivo bioengineering using an aortic matrix in a porcine model

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Abstract

Objective

To evaluate the feasibility of an in vivo small bowel bioengineering model using allogeneic aortic grafts in pigs.

Background

The best treatment for short bowel syndrome is still unclear. Intestinal transplantation, as well as lifelong parenteral nutrition is associated with a 5-year survival rate of less than 50 %. We have already used allogeneic arterial segments to replace the upper airway in sheep. The results were encouraging with an induced transformation of the aortic wall into tracheo-bronchial bronchial-type tissue.

Methods

Seven young mini-pigs were used. A 10-cm-diameter, allogeneic, aortic graft was interposed in an excluded small bowel segment and wrapped by the neighboring omentum. Animals were autopsied at 1 (n = 2), 3 (n = 3), and 6 months (n = 2), respectively. Specimens were examined macroscopically and microscopically.

Results

The overall survival rate of the animals was 71.4 %. No anastomotic leak occurred. Histologic analysis revealed intestinal-like wall transformation of the aortic graft in the surviving animals.

Conclusion

Aortic-enteric anastomosis is feasible in a porcine model. Moreover, in vivo, bioengineered, intestinal-like transformation of the vascular wall was identified.

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Authors and Affiliations

  1. Department of General and Minimally Invasive Surgery, Centre Hospitalier de Poissy/Saint-Germain-En-Laye, 10, rue du Champ Gaillard, 78300, Poissy, France

    Elie Chouillard & Elias Chahine

  2. Centre de Recherche Chirurgicale Dominique Chopin, Hôpital Henri Mondor, UPEC, Créteil, France

    Eric Allaire, Anne Filaire-Legendre & Jeanne Tran Van Nhieu

  3. Department of Thoracic and Vascular Surgery, Faculty of Medecine, SMBH Bobigny, Assistance Publique-Hôpitaux de Paris, Hôpitaux Universiatires Paris Seine-Saint-Deanis, Avicenne Hospital, Paris 13 University, Sorbonne-Paris Cité, Bobigny, France

    Emmanuel Martinod

  4. Alain Carpentier Foundation, Laboratory for Biosurgical Research, Georges Pompidou European Hospital, Paris Descartes University, Paris, France

    Emmanuel Martinod

Authors
  1. Elie Chouillard
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  2. Elias Chahine
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  3. Eric Allaire
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  4. Anne Filaire-Legendre
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  5. Jeanne Tran Van Nhieu
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  6. Emmanuel Martinod
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Corresponding author

Correspondence to Elie Chouillard.

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Disclosures

Elie Chouillard, Elias Chahine, Eric Allaire, Anne Filaire-Legendre, Jeanne Tran Van Nhieu, and Emmanuel Martinod have no conflicts of interest or financial ties to disclose

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Chouillard, E., Chahine, E., Allaire, E. et al. Small bowel in vivo bioengineering using an aortic matrix in a porcine model. Surg Endosc 30, 4742–4749 (2016). https://doi.org/10.1007/s00464-016-4815-z

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  • DOI: https://doi.org/10.1007/s00464-016-4815-z

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