Surgical Endoscopy

, Volume 29, Issue 6, pp 1445–1451 | Cite as

Development of a non-blurring, dual-imaging tissue marker for gastrointestinal tumor localization

  • Hideki Hayashi
  • Taro Toyota
  • Shoichi Goto
  • Aki Ooishi
  • Tao Gao
  • Lau Bik Ee
  • Hirosuke Hatayama
  • Tomonori Nomoto
  • Masanori Fujinami
  • Hisahiro Matsubara
Article

Abstract

Background

Knowing the exact location of gastrointestinal tumors both preoperatively and intraoperatively is essential for planning and performing laparoscopic surgery. Different techniques have been introduced to ascertain tumor locations during surgery, but none of these are fully satisfactory at establishing the minimum margins for organ resection while retaining curability. A new, non-blurring tissue marker, detectable by both X-ray computed tomography (CT) and near-infrared (NIR) fluorescence laparoscopy, has been developed, and we here examine its utility using an animal model.

Methods

Liposomes, comprised phospholipids and an NIR fluorescent dye (an indocyanine green derivative), and emulsions, consisting of phospholipids and oily radiographic contrast medium, were combined with polyglycerol-polyricinoleate to form giant cluster-like vesicles. This vesicular dispersion (300 μl) was administered into the porcine gastric submucosa using a gastroendoscope, and the detectability of the marker was examined using X-ray CT and NIR fluorescence laparoscopy.

Results

One hour after the administration of the vesicular dispersion, X-ray CT identified four individual injection sites, each at a 1-cm radius of a metal hemostasis clip. NIR fluorescence laparoscopy detected individual fluorescent spots 18 hours after the administration of the vesicular dispersion.

Conclusion

We anticipate that this newly developed tissue marker will contribute to the preoperative simulation of laparoscopic gastrointestinal cancer surgery and its intraoperative navigation.

Keywords

Indocyanine green Lipiodol® Near-infrared fluorescent dye Liposome Giant vesicle 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Hideki Hayashi
    • 1
    • 2
  • Taro Toyota
    • 3
    • 4
  • Shoichi Goto
    • 5
  • Aki Ooishi
    • 5
  • Tao Gao
    • 2
  • Lau Bik Ee
    • 6
  • Hirosuke Hatayama
    • 7
  • Tomonori Nomoto
    • 7
  • Masanori Fujinami
    • 7
  • Hisahiro Matsubara
    • 2
  1. 1.Center for Frontier Medical EngineeringChiba UniversityChibaJapan
  2. 2.Department of Frontier Surgery, Graduate School of MedicineChiba UniversityChibaJapan
  3. 3.Department of Basic Science, Graduate School of Arts and SciencesThe University of TokyoTokyoJapan
  4. 4.Department of Bioinformatics, Graduate School of MedicineChiba UniversityChibaJapan
  5. 5.Faculty of EngineeringChiba UniversityChibaJapan
  6. 6.Department of Medical System Engineering, Graduate School of EngineeringChiba UniversityChibaJapan
  7. 7.Department of Applied Chemistry and Biotechnology, Graduate School of EngineeringChiba UniversityChibaJapan

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