Abstract
Background
Carbon dioxide (CO2) has been used in the food industry as an antimicrobial agent. This study aimed to investigate whether CO2 pneumoperitoneum might act similarly as an antimicrobial agent in the infected peritoneal cavity.
Methods
Peritonitis was induced in 58 rats by intraabdominal injection of an Escherichia coli inoculum (6 × 105 colony-forming units [CFU]/ml). Control rats were injected with saline solution. The rats were randomly divided into four groups: rat control (RC, n = 15), bacterial inoculation control (BIC, n = 10), bacterial inoculation and laparotomy (BIL, n = 17), and bacterial inoculation and CO2 pneumoperitoneum (BIP, n = 16). The survival rates and histopathologic changes in the abdominal wall muscles, spleen, liver, intestines, and omentum were evaluated, and the samples were classified as “preserved” or “inflamed” (acute inflammation or tissue regeneration).
Results
The survival rates for the four groups were as follows: RC (100%), BIP (75%), BIL (53%), and BIC (30%). With regard to survival rates, statistically significant differences were observed between the following groups: RC and BIC (p = 0.0009), RC and BIL (p = 0.0045), BIP and BIC (p = 0.0332), and RC and BIP (p = 0.0470). No significant differences regarding survival rates were observed between the BIL and BIC groups or between the BIP and BIL groups. With regard to the number of inflamed samples per group, a statistically significant difference was observed between the BIC and RC groups and the BIL and RC groups (p = 0.05).
Conclusion
Carbon dioxide pneumoperitoneum has a protective effect against bacterial peritonitis induced in rats.
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Acknowledgments
We thank Fábio S. Goffi, Antonio Cláudio de Godoy, Renatto Andretto, Arnaldo Zanotto, and Nagamassa Yamagushi for their encouragement and support; Luci Ebisui and Raquel Guedes for technical support in the laboratory; Jeane G. Bronzatti for managing the supplies of the Hospital Alemão Oswaldo Cruz; Mr. and Mrs. Milton Ferraz Costa for managing the graphic composition; and the board of Endoview do Brasil Ltda.
Disclosures
Albino Augusto Sorbello, Joao Luiz Moreira Coutinho Azevedo, Junko Takano Osaka, Sueli Damy, Luiz Mattosinho França, and Erasmo Carlos Tolosa have no conflicts of interest or financial ties to disclose.
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Sorbello, A.A., Azevedo, J.L.M.C., Osaka, J.T. et al. Protective effect of carbon dioxide against bacterial peritonitis induced in rats. Surg Endosc 24, 1849–1853 (2010). https://doi.org/10.1007/s00464-010-0901-9
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DOI: https://doi.org/10.1007/s00464-010-0901-9