Surgical Endoscopy

, Volume 24, Issue 3, pp 578–583 | Cite as

Activation of nuclear factor kappa B and induction of migration inhibitory factor in tumors by surgical stress of laparotomy versus carbon dioxide pneumoperitoneum: an animal experiment

  • Anwar Tawfik AminEmail author
  • Norio Shiraishi
  • Shigeo Ninomiya
  • Masaaki Tajima
  • Masafumi Inomata
  • Seigo Kitano



Surgical trauma may be associated with enhanced tumor growth and establishment. The authors studied the effect of carbon dioxide (CO2) pneumoperitoneum versus laparotomy on tumor necrosis factor-α (TNFα), migration inhibitory factor (MIF) expression, and nuclear factor kappa B (NFκB) activity in human gastric cancer.


Nude mice were inoculated intraperitoneally with human gastric cancer cells (MKN45). Then laparotomy, CO2 pneumoperitoneum, and anesthesia alone were performed randomly. Tumor growth and associated TNFα and MIF expression and NFκB activity were determined.


Total tumor weight, especially at the anterior abdominal wall, was higher after laparotomy than after CO2 pneumoperitoneum (p < 0.05). The mRNA expression of TNFα was higher 24 and 48 h after laparotomy than after CO2 pneumoperitoneum (p < 0.05 and p < 0.01, respectively). At all the examined time points, MIF mRNA expression also was higher after laparotomy than after CO2 pneumoperitoneum (p < 0.05 until 1 week or p < 0.01 at 2 weeks). The NFκB protein was more activated after laparotomy than after CO2 pneumoperitoneum 6 h subsequent to surgical procedures.


After CO2 pneumoperitoneum, tumors have less TNFα and MIF expression and less NFκB activity than after laparotomy. This may be associated with less tumor growth, supporting minimal invasive techniques in gastrointestinal oncologic surgery.


Laparotomy MIF NFκPneumoperitoneum TNFα Tumor growth 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Anwar Tawfik Amin
    • 1
    • 2
    Email author
  • Norio Shiraishi
    • 1
  • Shigeo Ninomiya
    • 1
  • Masaaki Tajima
    • 1
  • Masafumi Inomata
    • 1
  • Seigo Kitano
    • 1
  1. 1.Department of Gastroenterological SurgeryOita University Faculty of MedicineOitaJapan
  2. 2.South Egypt Cancer Institute, Surgery DepartmentAssiut UniversityAssiutEgypt

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