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Serum leptin levels, hepatic leptin receptor transcription, and clinical predictors of non-alcoholic steatohepatitis in obese bariatric surgery patients

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Abstract

Background

Non-alcoholic steatohepatitis (NASH) is a major cause of liver disease in morbidly obese patients. Clinical predictors of NASH remain elusive, as do molecular mechanisms of pathogenesis.

Methods

A series of 35 morbidly obese patients undergoing bariatric surgery had a liver biopsy performed for standard histologic analysis. In addition, RNA was obtained from liver tissue and analyzed for leptin receptor gene expression. Regression analysis was used to correlate clinical variables, including serum leptin levels and hepatic leptin receptor gene expression, with the presence of histologically confirmed NASH.

Results

Of the 35 subjects enrolled, 29% had steatosis only, 60% had NASH, and 11% had normal liver histology. Among the clinical variables studied, only diabetes mellitus was an independent predictor of NASH. There was a trend toward lower levels of mRNA encoding the long form of the leptin receptor in hepatic tissue from patients with NASH compared to those with steatosis only.

Conclusions

Diabetes mellitus is associated with an increased risk of NASH in obese patients. Downregulation of hepatic leptin receptor may play a role in the pathogenesis of NASH.

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Acknowledgments

We thank Landon Donsbach for expert administrative assistance and manuscript preparation, and Vicki Jakovec and Susan Schenk for research coordination. R.W.O. receives support from the American Surgical Association, the Society of Advanced Gastrointestinal and Endoscopic Surgeons, and the Medical Research Foundation of Oregon.

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Authors and Affiliations

  1. Department of Surgery-L223A, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239

    D. Le, E. Lyle, B. S. Diggs, B. A. Jobe, T. Kay, C. W. Deveney, B. M. Wolfe & R. W. O’Rourke

  2. Department of Pathology, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239

    C. L. Corless

  3. Department of Pediatrics, Oregon Health & Science University, 3181 S.W. Sam Jackson Park Road, Portland, Oregon, 97239

    D. Marks & C. T. Roberts Jr.

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  1. D. Le
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Correspondence to R. W. O’Rourke.

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Le, D., Marks, D., Lyle, E. et al. Serum leptin levels, hepatic leptin receptor transcription, and clinical predictors of non-alcoholic steatohepatitis in obese bariatric surgery patients. Surg Endosc 21, 1593–1599 (2007). https://doi.org/10.1007/s00464-006-9185-5

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