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Immediate peritoneal response to bacterial contamination during laparoscopic surgery

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Abstract

Background

Several studies have shown that laparoscopic surgery (LS) minimizes surgical trauma and the immune function is better preserved. Another major advantage of LS is the lower incidence of septic complications. However, several in vitro studies have shown that CO2 severely impairs macrophage physiology. In theory, this would reduce the ability to respond to peritoneal contamination. However, there is some controversy in view of the evidence of a better preserved peritoneal response to sepsis. This study analyzed the early response of the peritoneum to contamination in a CO2 ambience.

Methods

A total of 192 CD-1 mice were distributed in three groups: group 1, laparotomy (LAP, n = 64); group 2, CO2 laparoscopy (CO2-LC, n = 64); and group 3, wall lift laparoscopy (WL-LC, n = 64). Mice in each group were randomized to receive 1 ml of Escherichia coli suspension (1 × 104 colony-forming units/ml) or saline. Peritoneal fluid was obtained at 1.5, 3, 6, and 12 h after surgery. Monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), and prostaglandin E2 (PGE2) were measured.

Results

MCP-1 levels were significantly greater and higher earlier in group 2 (CO2-LC) than in group 1 (LAP) (p < 0.007). Simultaneously, the increment in the traction group (WL-LC, group 3) was significantly higher (p < 0.002) than after laparotomy, with no differences in group 2 (CO2-LC). When a contamination was added to the laparotomy subgroup, there was a significant increase compared to the group without contamination (p < 0.5). MCP-1 modifications after contamination in the LAP group were statistically significant and appeared later than in the WL-LC (p < 0.002) and CO2-LC groups (p < 0.02). For IL-6, the three models presented a significant increase in the noncontaminated groups. This occurred significantly later in the LAP group. Simultaneously, the increase in IL-6 occurred earlier and was significantly higher in the WL-LC group compared to the LAP group (p < 0.003), without differences between CO2-LC and wall lift groups. Significant differences between contaminated and noncontaminated subgroups were only observed in the LC-CO2 groups. When contaminated, the traction model sustained a higher and earlier rise in IL-6 levels compared to the LAP and LC-CO2 groups (p < 0.001). For PGE2, The three models showed a significant increase in PGE2 levels in the noncontaminated groups. However, there were no significant differences between them. In the contaminated groups, there was no statistical difference between the groups.

Conclusion

Despite a transient impairment of the immediate peritoneal response to a septic challenge, the degree of injury with LS is lower than that with open surgery, and abdominal infection can therefore be better controlled.

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Acknowledgment

This work was supported by FIS grant 99/0341.

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Authors and Affiliations

  1. Service of Surgery, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain

    E. M. Targarona, M. Rodríguez, C. Balagué & M. Trias

  2. Laboratory of Inflammation Mediators, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain

    M. Camacho & L. Vila

  3. Epidemiology Unit, Hospital de la Santa Creu i Sant Pau, Universitat Autònoma de Barcelona, Barcelona, Spain

    I. Gich

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  1. E. M. Targarona
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  2. M. Rodríguez
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  3. M. Camacho
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  4. C. Balagué
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Correspondence to E. M. Targarona.

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Targarona, E.M., Rodríguez, M., Camacho, M. et al. Immediate peritoneal response to bacterial contamination during laparoscopic surgery. Surg Endosc 20, 316–321 (2006). https://doi.org/10.1007/s00464-005-0367-3

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