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Videoscopic fluorescence diagnosis of peritoneal and thoracic metastases from human hepatoblastoma in nude rats

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Abstract

Background

Various medical disciplines are employing photodynamic diagnosis (PDD) when searching for malignancies. It is still unknown whether pediatric solid tumors such as hepatoblastoma are susceptible to this technique as well.

Methods

Human hepatoblastoma cells were injected into the abdomen or right thoracic cavity of nude rats. Tumor growth was allowed for 7 weeks. Then, photosensitization was induced by peritoneal lavage with 5-aminolevulinic acid (ALA). Applying the Storz PDD system and one 4-mm scope, all animals were investigated by videoscopic white light diagnosis (WD) and PDD. Suspicious lesions were marked and analyzed by spectrometry and histology.

Results

Positive fluorescence was documented for every tumor seen by WD in the abdomen or right thoracic cavity. Spectrometry of lesions showed a 6.34-fold increased fluorescence intensity. Histology revealed hepatoblastoma in all specimens.

Conclusions

Human hepatoblastoma can be detected by PDD in a rat model. Considering the clinical success of this method in other specialties, our findings indicate that further investigations to evaluate the benefit of PDD for children with hepatoblastoma should be performed.

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References

  1. Doi I (1976) Establishment of a cell line and its clonal sublines from a patient with hepatoblastoma. Gann 67: 1–10

    PubMed  CAS  Google Scholar 

  2. Endlicher E, Messmann H (2004) Photodynamic diagnosis in the gastrointestinal tract. Gastrointest Endosc Clin North Am 14: 475–485

    Google Scholar 

  3. Fuchs J, Rydzynski J, von Schweinitz D, Bode U, Hecker H, Weinel P, Burger D, Harms D, Erttmann R, Oldhafer K, et al; Study Committee of the Cooperative Pediatric Liver Tumor Study Hb 94 for the German Society for Pediatric Oncology and Hematology (2002) Pretreatment prognostic factors and treatment results in children with hepatoblastoma: a report from the German Cooperative Pediatric Liver Tumor Study HB 94. Cancer 95: 172–182

    Article  PubMed  Google Scholar 

  4. Gahlen J, Pietschmann M, Prosst RL, Herfarth C (2001) Systemic vs. local administration of delta-aminolevulinic acid for laparoscopic fluorescence diagnosis of malignant intra-abdominal tumors. Experimental study. Surg Endosc 15: 196–199

    Article  PubMed  CAS  Google Scholar 

  5. Gahlen J, Prosst RL, Pietschmann M, Haase T, Rheinwald M, Skopp G, Stern J, Herfarth C (2002) Laparoscopic fluorescence diagnosis for intraabdominal fluorescence targeting of peritoneal carcinosis: experimental studies. Ann Surg 235: 252–260

    Article  PubMed  Google Scholar 

  6. Gahlen J, Prosst RL, Pietschmann M, Rheinwald M, Haase T, Herfarth C (1999) Spectrometry supports fluorescence staging laparoscopy after intraperitoneal aminolevulinic acid lavage for gastrointestinal tumors. J Photochem Photobiol B 52: 131–135

    Article  PubMed  CAS  Google Scholar 

  7. Gahlen J, Stern J, Pressmar J, Bohm J, Holle R, Herfarth C (2000) Local 5-aminolevulinic acid application for laser light-induced fluorescence diagnosis of early staged colon cancer in rats. Lasers Surg Med 26: 302–307

    Article  PubMed  CAS  Google Scholar 

  8. Haberle B, Bode U, von Schweinitz D (2003) Differentiated treatment protocols for high- and standard-risk hepatoblastoma—an interim report of the German Liver Tumor Study HB 99. Klin Padiatr 215: 159–165

    PubMed  CAS  Google Scholar 

  9. Loning M, Diddens H, Kupker W, Diedrich K, Huttmann G (2004) Laparoscopic fluorescence detection of ovarian carcinoma metastases using 5-aminolevulinic acid-induced protoporphyrin IX. Cancer 100: 1650–1656

    PubMed  Google Scholar 

  10. Ludicke F, Gabrecht T, Lange N, Wagnieres G, Van Den Bergh H, Berclaz L, Major AL (2003) Photodynamic diagnosis of ovarian cancer using hexaminolaevulinate: a preclinical study. Br J Cancer 88: 1780–1784

    PubMed  CAS  Google Scholar 

  11. Peng Q, Warloe T, Berg K, Moan J, Kongshaug M, Giercksky KE, Nesland JM (1997) 5-Aminolevulinic acid-based photodynamic therapy. Clinical research and future challenges. Cancer 79: 2282–2308

    Article  PubMed  CAS  Google Scholar 

  12. Prosst RL, Winkler S, Boehm E, Gahlen J (2002) Thoracoscopic fluorescence diagnosis (TFD) of pleural malignancies: experimental studies. Thorax 57: 1005–1009

    Article  PubMed  CAS  Google Scholar 

  13. Schnater JM, Köhler E, Bruder E, Bertschin S, Woodtli T, Pietsch T, von Schweinitz D, Lamers WH, Aronson DC (2002) An animal model for human hepatoblastoma. In: Siewert JR, Neugebauer E, Hartel W (eds) Chirurgisches forum 2002. Springer, Berlin Heidelberg

  14. Schnater JM, Kohler SE, Lamers WH, von Schweinitz D, Aronson DC (2003) Where do we stand with hepatoblastoma? A review. Cancer 98: 668–678

    Article  PubMed  CAS  Google Scholar 

  15. Zaak D, Kriegmair M, Stepp H, Baumgartner R, Oberneder R, Schneede P, Corvin S, Frimberger D, Knuchel R, Hofstetter A (2001) Endoscopic detection of transitional cell carcinoma with 5-aminolevulinic acid: results of 1012 fluorescence endoscopies. Urology 57: 690–694

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors thank N. Decker for her invaluable help. This work was supported by Karl Storz GmbH (equipment), Medac GmbH (ALA), and Prof. Dr. med. T. Pietsch (HuH 6 cells).

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Authors and Affiliations

  1. Department of Pediatric Surgery, Dr. V. Hauner Children’s Hospital, University of Munich, Lindwurmstrasse 4, 80337 , München, Germany

    H. Till, F. Bergmann, R. Metzger, B. Haeberle, K. Schaeffer & D. von Schweinitz

  2. Department of Surgery, University Hospital Mannheim, University of Heidelberg, Theodor-Kutzer-Ufer 1-3, 68167 , Mannheim, Germany

    R. L. Prosst

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  1. H. Till
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  2. F. Bergmann
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  3. R. Metzger
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  4. B. Haeberle
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  5. K. Schaeffer
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  6. D. von Schweinitz
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  7. R. L. Prosst
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Correspondence to F. Bergmann.

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Till, H., Bergmann, F., Metzger, R. et al. Videoscopic fluorescence diagnosis of peritoneal and thoracic metastases from human hepatoblastoma in nude rats. Surg Endosc 19, 1483–1486 (2005). https://doi.org/10.1007/s00464-005-0316-1

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  • DOI: https://doi.org/10.1007/s00464-005-0316-1

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