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Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1

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Abstract

Background

Abdominal mesothelial cells are exposed to carbon dioxide during laparoscopy. Previous data indicate that carbon dioxide increases release and expression of plasminogen activator inhibitor type-1 (PAI-1) and induces acidification.

Methods

To assess the impact resulting from a range of pH, human mesothelial cells were exposed to culturing media balanced to pH levels of 6.0 to 8.0 for 90 min. Samples from cell media were withdrawn at several time points. Concentrations of PAI-1 and PAI-1 activity were measured using enzyme-linked immunoassay techniques. To focus on the effect of clinically relevant pH, cells were subjected to pH 6.4 and 7.4. Samples were withdrawn for PAI-1 assessments and for PAI-1 mRNA analyses.

Results

During exposure to various levels of pH, PAI-1 secretion and activity were variable. However, 5 h after exposure, greater concentration and activity of PAI-1 were observed in acidified cultures. More PAI-1 mRNA was isolated after exposure of cells to a pH of 6.4, apparently indicating transcriptional regulation.

Conclusions

Mesothelial cells seem to respond to acidification by an increased release and production of PAI-1 in vitro.

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Acknowledgments

This study was supported financially by Gothenburg University and the Gothenburg Medical Society.

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Authors and Affiliations

  1. Department of Surgery, Sahlgrenska University Hospital, Göteborg University, S-41685, Göteborg, Sweden

    M. Bergström, P. Falk & L. Holmdahl

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  1. M. Bergström
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  2. P. Falk
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  3. L. Holmdahl
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Correspondence to M. Bergström.

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Bergström, M., Falk, P. & Holmdahl, L. Effect of acidosis on expression of mesothelial cell plasminogen activator inhibitor type-1. Surg Endosc 20, 1448–1452 (2006). https://doi.org/10.1007/s00464-005-0283-6

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  • DOI: https://doi.org/10.1007/s00464-005-0283-6

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