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A comparison of the effects of pneumoperitoneum and laparotomy on Natural Killer cell mediated cytotoxicity and Walker tumor growth in Wistar rats

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Abstract

Background

Surgical stress promotes impaired immunological function, which contributes to tumor growth. Natural killer activity (NKA) has a protective role in immunity to tumors. So, the aim of this experimental study was to assess tumor growth and (NKA) after pneumoperitoneum and laparotomy.

Methods

Sixty male Wistar rats were divided into three groups (anesthesia, CO2 pneumoperitoneum and laparotomy) plus ten controls. All experimental animals were inoculated subcutaneously with 8x105 Walker carcinosarcoma 256 cells. Animals were sacrificed on 1st(POD1) and 8th(POD8) postoperative day. Tumors were excised and weighed.

Results

On POD1 all animals had diminished NKA when compared to controls; NKA after pneumoperitoneum was significantly greater than after laparotomy. On POD8 all animals, except after laparotomy, reached NKA at controls levels. Tumor weight was significantly greater after laparotomy when compared to pneumoperitoneum.

Conclusions

Pneumoperitoneum causes a less depressed NKA and less tumor growth when compared to laparotomy.

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Authors and Affiliations

  1. Surgical Anatomy, Sao Paulo University, Sao Paulo, Brazil

    Paulo Emilio Fuganti, Aldo Junqueira Rodrigues Jr & Consuelo Junqueira Rodrigues

  2. Immunogenetics, Sao Paulo University, Sao Paulo, Brazil

    Maria Sato

Authors
  1. Paulo Emilio Fuganti
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  2. Aldo Junqueira Rodrigues Jr
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  3. Consuelo Junqueira Rodrigues
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  4. Maria Sato
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Correspondence to Paulo Emilio Fuganti.

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Fuganti, P.E., Rodrigues, A.J., Rodrigues, C.J. et al. A comparison of the effects of pneumoperitoneum and laparotomy on Natural Killer cell mediated cytotoxicity and Walker tumor growth in Wistar rats. Surg Endosc 20, 1858–1861 (2006). https://doi.org/10.1007/s00464-005-0189-3

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  • DOI: https://doi.org/10.1007/s00464-005-0189-3

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