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Effect of prolonged warm ischemia and pneumoperitoneum on renal function in a rat syngeneic kidney transplantation model

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Abstract

Background

Laparoscopic donor nephrectomy is associated with several advantages for the donor. However, graft function may be impaired due to use of pneumoperitoneum and prolonged warm ischemia. This study investigated the impact of pneumoperitoneum and prolonged warm ischemia on long-term graft function in a syngeneic rat renal transplant model.

Methods

A total of 27 Brown Norway rats were randomized for transplantation of kidneys after three different procedures: no insufflation and no warm ischemia (group 1), no insufflation with 20 min of warm ischemia (group 2), and CO2 insufflation and 20 min of warm ischemia (group 3). Glomerular filtration rate (GRF), serum creatinine, urine volume, urine creatinine, and proteinuria were determined monthly for 1 year. One year after transplantation, the grafts were removed for histomorphologic analysis.

Results

No significant differences in GRF, serum creatinine, urine volume, and proteinuria were found among the three groups. Histologic analysis also showed no differences between the groups.

Conclusion

Warm ischemia in combination with CO2 pneumoperitoneum, as used in laparoscopic donor nephrectomy, does not result in a negative effect on long-term graft function.

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Acknowledgment

The authors thank N. Ramlakhan for her help with sample collection.

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Authors and Affiliations

  1. Department of Surgery, Erasmus MC, Dr. Molewaterplein 40, 3015, GD, Rotterdam, The Netherlands

    M. Y. Lind, E. J. Hazebroek, F. Bonthuis, R. W. F. de Bruin & J. N. M. Ijzermans

  2. Department of Pathology, Leiden University Medical Center, Albinusdreef 2, 2333, ZA, Leiden, The Netherlands

    I. M. Bajema

  3. Department of Biostatistics and Epidemiology, Erasmus MC, Dr. Molewaterplein 50, 3015, GE, Rotterdam, The Netherlands

    W. C. J. Hop

Authors
  1. M. Y. Lind
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  2. E. J. Hazebroek
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  3. I. M. Bajema
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  4. F. Bonthuis
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  5. W. C. J. Hop
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  6. R. W. F. de Bruin
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  7. J. N. M. Ijzermans
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Corresponding author

Correspondence to J. N. M. Ijzermans.

Additional information

Paper presented at the annual meeting of the European Association for Endoscopic Surgery (EAES), 2004.

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Lind, M.Y., Hazebroek, E.J., Bajema, I.M. et al. Effect of prolonged warm ischemia and pneumoperitoneum on renal function in a rat syngeneic kidney transplantation model. Surg Endosc 20, 1113–1118 (2006). https://doi.org/10.1007/s00464-005-0050-8

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  • DOI: https://doi.org/10.1007/s00464-005-0050-8

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