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Port-site metastasis after CO2 pneumoperitoneum

Role of adhesion molecules and prevention with antiadhesion molecules

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Abstract

Background

Port-site metastasis is a continuing problem in laparoscopic cancer surgery. To clarify the role of adhesion molecules in the development of port-site metastasis, particularly with regard to prevention, we performed experiments in which port-site metastasis was inhibited using antibodies against extracellular matrix proteins or the active Arg–Gly–Asp (RGD) peptide after CO2 pneumoperitoneum in a murine model.

Methods

We examined the development of port-site metastasis under the following conditions: (1) CO2 pneumoperitoneum with or without hyaluronic acid and anti-integrin or anti-CD44 antibody and (2) CO2 pneumoperitoneum and a RGD peptide or pseudo-RGD sequence peptide (FC-336). BALB/c mice (n = 130) were injected with 5 × 105 human gastric cancer cells (MKN45) and either antibody or peptide, treated with CO2 pneumoperitoneum, and injected intraperitoneally with antibody or peptide for 5 days. Three weeks after CO2 pneumoperitoneum, the frequency and weight of port-site metastatic tumors were determined.

Results

Anti-integrin antibody significantly decreased the weight of port-site metastatic tumors without hyaluronic acid (control vs anti-integrin: 8.2 ± 7.1 vs 3.6 ± 4.5 mg; p < 0.05) but not the frequency of port-site metastases. With hyaluronic acid, the frequency of port-site metastasis and the weight of port-site metastatic tumors were significantly decreased both by anti-integrin and by anti-CD44 antibody (control vs anti-integrin and anti-CD44; 95% and 8.5 ± 7.2 mg vs 50% and 3.1 ± 4.3 mg and 55% and 3.3 ± 5.1 mg, respectively; p < 0.05). RGD peptide and FC-336 also inhibited port-site metastasis in a dose-dependent manner.

Conclusion

Cell adhesion molecules integrin and CD44 play an important role in the development of port-site metastasis after laparoscopic cancer surgery. Intraperitoneal injection of RGD peptide or pseudo-RGD sequence peptide (FC-336) can prevent port-site metastasis.

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Authors and Affiliations

  1. Department of Surgery I, Oita Medical University, 1-1 Idaigaoka, 879-5593, Hasama-machi, Oita, Japan

    Y. Hirabayashi, N. Shiraishi, Y. Adachi & S. Kitano

  2. Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University, Toyama, Japan

    K. Yamaguchi & I. Saiki

Authors
  1. Y. Hirabayashi
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  2. K. Yamaguchi
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  3. N. Shiraishi
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  4. Y. Adachi
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  5. I. Saiki
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  6. S. Kitano
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Corresponding author

Correspondence to S. Kitano.

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Hirabayashi, Y., Yamaguchi, K., Shiraishi, N. et al. Port-site metastasis after CO2 pneumoperitoneum. Surg Endosc 18, 1113–1117 (2004). https://doi.org/10.1007/s00464-003-9150-5

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  • DOI: https://doi.org/10.1007/s00464-003-9150-5

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