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Dysphagia

, Volume 33, Issue 5, pp 627–635 | Cite as

Maturation of the Coordination Between Respiration and Deglutition with and Without Recurrent Laryngeal Nerve Lesion in an Animal Model

  • Ashley Ballester
  • François Gould
  • Laura Bond
  • Bethany Stricklen
  • Jocelyn Ohlemacher
  • Andrew Gross
  • Katherine DeLozier
  • Randall Buddington
  • Karyl Buddington
  • Nicole Danos
  • Rebecca GermanEmail author
Original Article

Abstract

The timing of the occurrence of a swallow in a respiratory cycle is critical for safe swallowing, and changes with infant development. Infants with damage to the recurrent laryngeal nerve, which receives sensory information from the larynx and supplies the intrinsic muscles of the larynx, experience a significant incidence of dysphagia. Using our validated infant pig model, we determined the interaction between this nerve damage and the coordination between respiration and swallowing during postnatal development. We recorded 23 infant pigs at two ages (neonatal and older, pre-weaning) feeding on milk with barium using simultaneous high-speed videofluoroscopy and measurements of thoracic movement. With a complete linear model, we tested for changes with maturation, and whether these changes are the same in control and lesioned individuals. We found (1) the timing of swallowing and respiration coordination changes with maturation; (2) no overall effect of RLN lesion on the timing of coordination, but (3) a greater magnitude of maturational change occurs with RLN injury. We also determined that animals with no surgical intervention did not differ from animals that had surgery for marker placement and a sham procedure for nerve lesion. The coordination between respiration and swallowing changes in normal, intact individuals to provide increased airway protection prior to weaning. Further, in animals with an RLN lesion, the maturation process has a larger effect. Finally, these results suggest a high level of brainstem sensorimotor interactions with respect to these two functions.

Keywords

Deglutition Respiration Recurrent laryngeal nerve Sensorimotor Infant Development Animal model 

Notes

Acknowledgements

The Authors thank the CMU at NEOMED for their extensive support of this project.

Funding

This study was funded by NIH 1R01HD088561 to RZG.

Compliance with Ethical Standards

Conflict of interest

All authors declare they have no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed, and was done in accordance with NEOMED IACUC approval, protocol #17-04-071.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Ashley Ballester
    • 1
    • 5
  • François Gould
    • 1
  • Laura Bond
    • 1
  • Bethany Stricklen
    • 1
  • Jocelyn Ohlemacher
    • 1
  • Andrew Gross
    • 1
  • Katherine DeLozier
    • 1
  • Randall Buddington
    • 2
  • Karyl Buddington
    • 3
  • Nicole Danos
    • 4
  • Rebecca German
    • 1
    Email author
  1. 1.Department of Anatomy and NeurobiologyNEOMEDRootstownUSA
  2. 2.School of Health StudiesUniversity of MemphisMemphisUSA
  3. 3.Department of Biological SciencesUniversity of MemphisMemphisUSA
  4. 4.University of San DiegoSan DiegoUSA
  5. 5.Division of Obstetrics and GynecologyAkron General HospitalAkronUSA

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