Some studies on optimization of extraction process for protease production in SSF

Abstract

A locally isolated filamentous fungus belonging to the group phycomycetes namely Rhizopus oryzae was identified to secrete alkaline protease. The production of this enzyme through solid state fermentation process has been attempted. From fermented biomass extraction of the enzyme was found to depend on the different parameters like nature of solvent, time of soaking, temperature etc. While optimizing the extraction process, it was found that 10% ethanol with 3% glycerol was the best solvent for protease extraction, when the soaking time was 2 hours and temperature 30 °C. It was further observed that double wash of fermented biomass yielded almost total enzyme in the leachate.