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High yield chromatographic purification of enzymatically produced tyrosine fructosyl ester

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Abstract

The synthesis of L-tyrosine fructosyl ester, from fructose and L-tyrosine methyl ester, was carried out by a transesterification reaction catalyzed by α-chymotrypsin in water without any organic cosolvent. The yield was optimized by regulating the pH of the reaction medium and a maximum yield of 63% was obtained.

A two-step process of tyrosine fructosyl ester purification was proposed using adsorbent resin and activated carbon. Different elutions and supports easily applicable to industrial process have been studied. Solutions of tyrosine fructosyl ester with a 96% purity and 70% recovery yield were obtained by chromatography on a column of acrylic polyester resins with a solution of 0.5 M NaCl as eluant.

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Authors and Affiliations

  1. Laboratoire de Biotechnologie-Bioprocédés (UMR CNRS 5504, LA INRA), Centre de Bioingénierie Gilbert Durand, INSA, Complexe scientifique de Rangueil, 31077 Toulouse Cedex, France, , , , , , FR

    X. Cameleyre & D. Combes

  2. Eridania Béghin-Say, Laboratoire de Recherche Toulouse, , , , ,

    J. M. Taconnat & A. Guibert

Authors
  1. X. Cameleyre
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  2. J. M. Taconnat
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  3. A. Guibert
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  4. D. Combes
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Received: 9 June 1997

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Cameleyre, X., Taconnat, J., Guibert, A. et al. High yield chromatographic purification of enzymatically produced tyrosine fructosyl ester. Bioprocess Engineering 18, 361–366 (1998). https://doi.org/10.1007/s004490050456

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  • DOI: https://doi.org/10.1007/s004490050456

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